Organization and Expression of the <i>Bacillus subtilis sigY</i> Operon

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  • Tojo Shigeo
    Department of Biotecnology, Faculty of Life Science and Biotechnology, Fukuyama University
  • Matsunaga Masayuki
    Department of Biotecnology, Faculty of Life Science and Biotechnology, Fukuyama University
  • Matsumoto Takashi
    Departement of Biochemistry and Molecular Biology, Faculty of Science, Saitama University
  • Kang Choong-Min
    Department of Biotecnology, Faculty of Life Science and Biotechnology, Fukuyama University Choong-MIn Kang, Children's Hospital
  • Yamaguchi Hirotake
    Department of Biotecnology, Faculty of Life Science and Biotechnology, Fukuyama University
  • Asai Kei
    Departement of Biochemistry and Molecular Biology, Faculty of Science, Saitama University
  • Sadaie Yoshito
    Departement of Biochemistry and Molecular Biology, Faculty of Science, Saitama University
  • Yoshida Ken-ichi
    Department of Biotecnology, Faculty of Life Science and Biotechnology, Fukuyama University
  • Fujita Yasutaro
    Department of Biotecnology, Faculty of Life Science and Biotechnology, Fukuyama University

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  • Organization and Expression of the Bacillus subtilis sigY Operon

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We investigated the organization and expression of the Bacillus subtilis sigY operon, the first gene of which codes for σY, a member of the extracytoplasmic function (ECF) family of sigma factors. The sigY operon, comprising six genes (sigY, yxlC, D, E, F, and G), was induced upon nitrogen starvation; it was continuously transcribed from the 31 st base upstream of sigY to a neighboring convergent gene, yxlH, resulting in a 4.2-kb mRNA. The expression of the sigY operon was also positively autoregulated through σY, suggesting that its transcription is likely to be directed by σY. Deletion analysis of the sigY promoter, which was localized by primer extension, revealed the promoter region of sigY with the “-10” and “-35” sequences of CGTC and TGAACG, respectively. The latter sequence was distinct from those recognized by σW, σX, and σM. The σY-directed transcription of sigY was under negative regulation involving YxlD. sigY disruption affected sporulation induced by nitrogen starvation, but sigY induction upon nitrogen starvation was not associated with the sporulation process. The organization and function of the sigY operon are significantly conserved in several microorganisms living in adverse living environments.

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