{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282679965296384.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.15036/arerugi.53.423"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"026075556"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/026075556"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I026075556"}},{"identifier":{"@type":"NAID","@value":"110002404785"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/2004213491"}}],"dc:title":[{"@language":"ja","@value":"気道上皮細胞BEAS-2BにおけるEotaxinの発現と各種グルココルチコイド薬による抑制効果の比較検討"},{"@language":"en","@value":"COMPARISON OF THE INHIBITORY EFFECTS OF GLUCOCORTICOIDS, ON THE EXPRESSION OF EOTAXIN IN AIRWAY EPITHELIAL CELL LINE BEAS-2B"},{"@language":"ja-Kana","@value":"キドウ ジョウヒ サイボウ BEAS-2B ニ オケル Eotaxin ノ ハツゲン ト カクシュ グルココルチコイドヤク ニ ヨル ヨクセイ コウカ ノ ヒカク ケントウ"}],"dc:language":"ja","description":[{"type":"abstract","notation":[{"@language":"en","@value":"Objective : Inhaled corticosteroids play a pivotal role in the treatment of asthma. To observe the mechanisms of glucocorticoids, we focused our study on the comparison of several glucocorticoids' effects on eotaxin expression in the airway epithelial cells. Methods : Airway epithelial cell line BEAS-2B was cultured in vitro. Cells were preincubated with or without glucocorticoids (becromethasone dipropionate ; BDP, budesonide ; BUD, fluticasone propionate ; FP) and stimulated with TNF_α and/or IL-4. Protein levels of eotaxin in the supernatants of the cultured cells were determined by ELISA. Results and Conclusions : TNF_α and IL-4 increased the levels of eotaxin in BEAS-2B cells. Combination of these cytokines synergistically upregulated the eotaxin expression as reported previously. Each glucocorticoid significantly inhibited the expression of eotaxin protein induced with TNF_α and IL-4 and the compared efficacy was in order of FP>BUD>BDP. FP seemed most potent and the inhibitory effect was also observed with relatively low concentration such as 10^<-10>M. Taken together, the comparison of the potency of each glucocorticoid using airway epithelial cells may reflect the efficacy of these drugs in asthmatics."},{"@language":"ja","@value":"(研究目的)喘息治療の中心的役割を果たす吸入ステロイドの作用を比較検討するため,気道上皮細胞におけるeotaxinの産生に対する効果を検討した.(方法)気道上皮細胞株BEAS-2Bをin vitroにて培養し,各種glucocorticoids(beclomethasone dipropionate ; BDP, budesonide ; BUD, fluticasone Dropionate ; FP)を添加し,TNF_αおよびIL-4にて刺激,培養上清中のeotaxinタンパク濃度をELISAにて測定した.(結果・考察)TNF_αおよびIL-4は,それぞれ濃度・時間依存性にBEAS-2B細胞におけるeotaxin産生を増強し,また両者には相乗効果が認められた.Glucocorticoidsは,これらの刺激によるeotaxin産生を有意に抑制し,その力価は,FP>BUD>BDPの順で,FPには10^<-10>Mという低濃度でも有意な抑制効果が認められた.これらの結果は,臨床比較試験の結果と相関することが示唆された."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420282801205066880","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"10384231"},{"@type":"NRID","@value":"1000010384231"},{"@type":"NRID","@value":"9000002892087"},{"@type":"NRID","@value":"9000021058605"},{"@type":"NRID","@value":"9000258296181"},{"@type":"NRID","@value":"9000368417380"},{"@type":"NRID","@value":"9000345218168"},{"@type":"NRID","@value":"9000391421887"},{"@type":"NRID","@value":"9000384274601"},{"@type":"NRID","@value":"9000020319449"},{"@type":"NRID","@value":"9000356503745"}],"foaf:name":[{"@language":"ja","@value":"松倉 聡"},{"@language":"en","@value":"Matsukura Satoshi"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"昭和大学医学部第一内科"},{"@language":"en","@value":"First Department of Internal Medicine, Showa University School of Medicine"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679965296389","@type":"Researcher","foaf:name":[{"@language":"ja","@value":"國分 二三男"},{"@language":"en","@value":"Kokubu Fumio"}],"jpcoar:affiliationName":[{"@language":"en","@value":"First Department of Internal Medicine, Showa University School of Medicine"},{"@language":"ja","@value":"昭和大学医学部第一内科"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679965296387","@type":"Researcher","foaf:name":[{"@language":"ja","@value":"黒川 真嗣"},{"@language":"en","@value":"Kurokawa Masatsugu"}],"jpcoar:affiliationName":[{"@language":"en","@value":"First Department of Internal Medicine, Showa University School of Medicine"},{"@language":"ja","@value":"昭和大学医学部第一内科"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658538682368","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"50365748"},{"@type":"NRID","@value":"1000050365748"},{"@type":"NRID","@value":"9000010426431"},{"@type":"NRID","@value":"9000258296179"},{"@type":"NRID","@value":"9000001211760"},{"@type":"NRID","@value":"9000391658435"},{"@type":"NRID","@value":"9000413523640"},{"@type":"NRID","@value":"9000240058314"},{"@type":"NRID","@value":"9000273019620"},{"@type":"NRID","@value":"9000257947378"},{"@type":"NRID","@value":"9000413479361"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0140721"}],"foaf:name":[{"@language":"ja","@value":"川口 未央"},{"@language":"en","@value":"Kawaguchi Mio"}],"jpcoar:affiliationName":[{"@language":"en","@value":"First Department of Internal Medicine, Showa University School of Medicine"},{"@language":"ja","@value":"昭和大学医学部第一内科"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001204985243011","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000003745976"}],"foaf:name":[{"@language":"ja","@value":"渡辺 伸"},{"@language":"en","@value":"Watanabe Shin"}],"jpcoar:affiliationName":[{"@language":"en","@value":"First Department of Internal Medicine, Showa University School of Medicine"},{"@language":"ja","@value":"昭和大学医学部第一内科"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679965296395","@type":"Researcher","foaf:name":[{"@language":"ja","@value":"久賀 秀樹"},{"@language":"en","@value":"Kuga Hideki"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"昭和大学医学部第一内科:高木総合病院内科"},{"@language":"en","@value":"First Department of Internal Medicine, Showa University School of Medicine : Internal Medicine, Takagi General Hospital"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679965296385","@type":"Researcher","foaf:name":[{"@language":"ja","@value":"鈴木 慎太郎"},{"@language":"en","@value":"Suzuki Shintaro"}],"jpcoar:affiliationName":[{"@language":"en","@value":"First Department of Internal Medicine, Showa University School of Medicine"},{"@language":"ja","@value":"昭和大学医学部第一内科"}]},{"@id":"https://cir.nii.ac.jp/crid/1030285133827961603","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"10095870"},{"@type":"NRID","@value":"1000010095870"},{"@type":"NRID","@value":"9000020324007"},{"@type":"NRID","@value":"9000253592718"},{"@type":"NRID","@value":"9000252909194"},{"@type":"NRID","@value":"9000256138610"},{"@type":"NRID","@value":"9000020013625"},{"@type":"NRID","@value":"9000019976540"},{"@type":"NRID","@value":"9000021066325"},{"@type":"NRID","@value":"9000253619419"},{"@type":"NRID","@value":"9000020463795"},{"@type":"NRID","@value":"9000020358455"},{"@type":"NRID","@value":"9000020025619"},{"@type":"NRID","@value":"9000254179160"},{"@type":"NRID","@value":"9000006217899"},{"@type":"NRID","@value":"9000021627307"},{"@type":"NRID","@value":"9000258296180"},{"@type":"NRID","@value":"9000255736824"},{"@type":"NRID","@value":"9000020047719"},{"@type":"NRID","@value":"9000020996855"},{"@type":"NRID","@value":"9000253630375"},{"@type":"NRID","@value":"9000283334721"},{"@type":"NRID","@value":"9000020671946"},{"@type":"NRID","@value":"9000020094164"},{"@type":"NRID","@value":"9000253623261"},{"@type":"NRID","@value":"9000003714057"},{"@type":"NRID","@value":"9000020427515"},{"@type":"NRID","@value":"9000020358495"},{"@type":"NRID","@value":"9000019976559"},{"@type":"NRID","@value":"9000020994755"},{"@type":"NRID","@value":"9000020946766"},{"@type":"NRID","@value":"9000255736428"},{"@type":"NRID","@value":"9000020995583"},{"@type":"NRID","@value":"9000021651307"},{"@type":"NRID","@value":"9000256139567"},{"@type":"NRID","@value":"9000258295963"},{"@type":"NRID","@value":"9000001032681"},{"@type":"NRID","@value":"9000000877796"},{"@type":"NRID","@value":"9000020402262"},{"@type":"NRID","@value":"9000021650469"},{"@type":"NRID","@value":"9000253613798"},{"@type":"NRID","@value":"9000257777149"},{"@type":"NRID","@value":"9000018392778"},{"@type":"NRID","@value":"9000283212509"},{"@type":"NRID","@value":"9000008352084"},{"@type":"NRID","@value":"9000020950614"},{"@type":"NRID","@value":"9000256139179"},{"@type":"NRID","@value":"9000325822049"},{"@type":"NRID","@value":"9000003758526"},{"@type":"NRID","@value":"9000021096123"},{"@type":"NRID","@value":"9000253615667"},{"@type":"NRID","@value":"9000009357163"},{"@type":"NRID","@value":"9000020022465"},{"@type":"NRID","@value":"9000283334867"},{"@type":"NRID","@value":"9000018367390"},{"@type":"NRID","@value":"9000020402134"},{"@type":"NRID","@value":"9000021642077"},{"@type":"NRID","@value":"9000020937143"},{"@type":"NRID","@value":"9000020905879"},{"@type":"NRID","@value":"9000020945472"},{"@type":"NRID","@value":"9000252909770"},{"@type":"NRID","@value":"9000255942178"}],"foaf:name":[{"@language":"ja","@value":"足立 満"},{"@language":"en","@value":"Adachi Mitsuru"}],"jpcoar:affiliationName":[{"@language":"en","@value":"First Department of Internal Medicine, Showa University School of Medicine"},{"@language":"ja","@value":"昭和大学医学部第一内科"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679965296390","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Schleimer Robert P."}],"jpcoar:affiliationName":[{"@language":"en","@value":"Johns Hopkins Asthma and Allergy Center"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679965296394","@type":"Researcher","foaf:name":[{"@language":"ja","@value":"尾高 美保"},{"@language":"en","@value":"Odaka Miho"}],"jpcoar:affiliationName":[{"@language":"en","@value":"First Department of Internal Medicine, Showa University School of Medicine"},{"@language":"ja","@value":"昭和大学医学部第一内科"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679965296384","@type":"Researcher","foaf:name":[{"@language":"ja","@value":"竹内 ひろ子"},{"@language":"en","@value":"Takeuchi Hiroko"}],"jpcoar:affiliationName":[{"@language":"en","@value":"First Department of Internal Medicine, Showa University School of Medicine"},{"@language":"ja","@value":"昭和大学医学部第一内科"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282679965296391","@type":"Researcher","foaf:name":[{"@language":"ja","@value":"家城 光志"},{"@language":"en","@value":"Ieki Koushi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"First Department of Internal Medicine, Showa University School of Medicine"},{"@language":"ja","@value":"昭和大学医学部第一内科"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00214884"},{"@type":"LISSN","@value":"00214884"},{"@type":"EISSN","@value":"13477935"},{"@type":"NDL_BIB_ID","@value":"000000001300"},{"@type":"ISSN","@value":"00214884"},{"@type":"NCID","@value":"AN00012583"}],"prism:publicationName":[{"@language":"ja","@value":"アレルギー"},{"@language":"en","@value":"Japanese Journal of Allergology"},{"@language":"ja","@value":"アレルギー"},{"@language":"en","@value":"Japanese Journal of Allergology"}],"dc:publisher":[{"@language":"en","@value":"Japanese Society of Allergology"},{"@language":"ja","@value":"一般社団法人 日本アレルギー学会"}],"prism:publicationDate":"2004","prism:volume":"53","prism:number":"4","prism:startingPage":"423","prism:endingPage":"429"},"url":[{"@id":"http://id.ndl.go.jp/bib/026075556"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I026075556"},{"@id":"https://search.jamas.or.jp/link/ui/2004213491"}],"availableAt":"2004","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=airway%20epithelial%20cell","dc:title":"airway epithelial cell"},{"@id":"https://cir.nii.ac.jp/all?q=bronchial%20asthma","dc:title":"bronchial asthma"},{"@id":"https://cir.nii.ac.jp/all?q=eotaxin","dc:title":"eotaxin"},{"@id":"https://cir.nii.ac.jp/all?q=glucocorticoid","dc:title":"glucocorticoid"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360292620972033664","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Differential regulation of eotaxin expression by IFN-γ in airway epithelial cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574094127435648","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Eotaxin and monocyte chemotactic protein-4 mRNA expression in small airways of asthmatic and nonasthmatic individuals"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044951032192","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Eotaxin: a potent eosinophil chemoattractant cytokine detected in a guinea pig model of allergic airways inflammation."}]},{"@id":"https://cir.nii.ac.jp/crid/1362262945001683840","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Enhanced expression of eotaxin and CCR3 mRNA and protein in atopic asthma. Association with airway hyperresponsiveness and predominant co‐localization of eotaxin mRNA to bronchial epithelial and endothelial cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544418621932928","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Constitutive and allergen-induced expression of eotaxin mRNA in the guinea pig lung."}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679962512768","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"485 ヒト気管支平滑筋細胞におけるケモカインの発現とステロイドの効果"}]},{"@id":"https://cir.nii.ac.jp/crid/1570009751167095296","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Clinical efficacy of budesonide Turbuhaler compared with that of beclomethasone dipropionate pMDI with volumatic spacer. A 2-year randomized study in 102 asthma patients"}]},{"@id":"https://cir.nii.ac.jp/crid/1570009751736358400","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Transformation of human bronchial epithelial cells by infection with SV40 or adenovirus-12 SV40 hybrid virus, or transfection via strontium phosphate coprecipitation with a plasmid containing SV40 early region genes"}]},{"@id":"https://cir.nii.ac.jp/crid/1570009751800391680","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Eosinophil chemotactic chemokines (eotaxin, eotaxin-2. RANTES, monocyte chemoattractant protein-3 (MCP-3), and MCP-4). and C-C chemokine receptor 3 expression in bronchial biopsies from atopic and nonatopic (intrinsic) asthmatics"}]},{"@id":"https://cir.nii.ac.jp/crid/1570291226770886400","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1570854175112800512","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1571135650089511680","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Part 1. Pharmacokinetic/pharmacodynamic comparison of the inhaled corticosteroids"}]},{"@id":"https://cir.nii.ac.jp/crid/1571135651707577856","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"IL-4 induces eotaxin : a possible mechanism of selective eosinophil recruitment in helminth infection and atopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1571417125066221312","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Clinical experience with fluticasone propionate in asthma ; a meta-analysis of efficacy and systemic activity compared with budesonide and beclomethasone dipropionate at half the microgram dose of less"}]},{"@id":"https://cir.nii.ac.jp/crid/1571698601345348480","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"An in vitro comparison of commonly used topical glucocorticoid preparations"}]},{"@id":"https://cir.nii.ac.jp/crid/1571980075019643520","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Interleukin-13 upregulates eotaxin expression in airway epithelial cells by a STAT6-dependent mechanism"}]},{"@id":"https://cir.nii.ac.jp/crid/1572261551298697984","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The inhibitory effects on IL-4, IL-5, and interferon-γ production by cultured primary CD4^+ T cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1572543024973063936","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Fluticasone propionate and budesonide in adult asthmatics ; a comparison using dry-powder inhaler devices"}]},{"@id":"https://cir.nii.ac.jp/crid/1572543024973065216","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Activation of eotaxin gene transcription by NF-κB and STAT6 in human airway epithelial cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1573668924879906560","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1573950399812887040","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1573950399856615552","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Expression of eotaxin by human lung epithelial cells ; induction by cytokines and inhibition by glucocorticoids"}]}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:2001820484"},{"@type":"NDL_SEARCH","@value":"oai:ndlsearch.ndl.go.jp:R000000004-I026075556"},{"@type":"CIA","@value":"110002404785"}]}