{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282680026294656.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.11150/kansenshogakuzasshi.87.431"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"024819888"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/024819888"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I024819888"}},{"identifier":{"@type":"PMID","@value":"23984592"}},{"identifier":{"@type":"NAID","@value":"10031201445"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/2013351723"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Evaluation of an Immunochromatographic Fourth Generation Test for the Rapid Diagnosis of Acute HIV Infection"},{"@language":"ja","@value":"HIV 急性感染期の診断における第 4 世代 HIV 迅速検査試薬の性能評価"},{"@language":"ja-Kana","@value":"HIV キュウセイ カンセンキ ノ シンダン ニ オケル ダイ4 セダイ HIV ジンソク ケンサ シヤク ノ セイノウ ヒョウカ"}],"dc:language":"ja","description":[{"type":"abstract","notation":[{"@language":"en","@value":"The early diagnosis of human immunodeficiency virus (HIV) infection is important to provide effective antiviral treatment and to prevent transmission of HIV. One of the key issues to achieve this goal is to shorten the so-called “diagnostic window period” when the humoral immune response toward the virus is not fully developed during the acute phase of HIV-1 infection. In 2008, the Espline HIV Ag/Ab test kit (E4G, Fujirebio Inc. Japan) was marketed in Japan belonging to the fourth generation of HIV test kits characterized by its ability to detect both viral antigens (Ag) and anti-HIV-1/2 antibodies (Ab). E4G is the first and only fourth generation immunochromatographic HIV test kit approved in Japan at present. To evaluate its performance to diagnose acute HIV infection (AHI), E4G was compared with fourth generation Ag/Ab ELISA test kits, a third generation PA test kit, WB and real-time PCR for the testing of 25 AHI clinical specimens. E4G detected HIV infection in 18/25 specimens (sensitivity : 72.0％), of which the viral Ag was detected in only 2 specimens (8.0％) bearing a viral load＞10 million copies/mL. No spesimens were simultaneously reactive to both Ag and Ab against HIV. The third generation PA achieved a positive score of 17/25 specimens (68.0％), which was almost the same as the E4G figure. In contrast the fourth generation Ag/Ab ELISA scored all the 25 AHI specimens positive (sensitivity : 100％). Overall, although having the merit of offering a rapid diagnostic test for HIV infection, E4G does not provide a sensitivity in AHI diagnosis superior to test kits currently available."},{"@language":"ja","@value":"Human immunodeficiency virus（HIV）の早期診断は効果的な治療提供と感染拡大阻止のために重要で ある．HIV の p24 抗原を抗体と同時に検出する第 4 世代検査法は，HIV 急性感染で問題となる抗体陽転化までのウインドウ期を短縮するために有用と考えられる．2008 年 11 月にイムノクロマトグラフィーの原理を応用し迅速性にすぐれた第 4 世代の体外診断用医薬品としてエスプライン HIV Ag/Ab（富士レビオ）が本邦で承認された．我々はエスプライン HIV Ag/Ab の HIV 急性感染期における検査性能を評価するため，第 4 世代 ELISA 法，第 3 世代 PA 法，WB 法および核酸増幅法と比較した．25 例の急性感染期患者検体を検査したところ，エスプライン HIV Ag/Ab は 18 例（72％）を陽性と判定し，他の検査は第 4 世代 ELISA 法 25 例（100％），第 3 世代 PA 法 17 例（68％），核酸増幅法 25 例（100％）であった．WB 法では 7 例が陰性，18 例が判定保留であった．エスプライン HIV Ag/Ab で陽性となった 18 例のうち，16 例（64％）は抗体のみに反応した．抗原に反応したものは 2 例（8％）で，それらのウイルスコピー数は共に 10<sup>7</sup> コピー/mL 以上であった．これは非常に高いレベルのウイルス血症を呈した場合にのみエスプライン HIV Ag/Ab で抗原が検出可能であることを示唆する．以上をまとめると，エスプライン HIV Ag/Ab の急性感染期における検出感度は第 4 世代 ELISA 法よりも低く，第 3 世代 PA 法と同程度であり，急性 HIV 感染診断への応用には十分な検討が必要と思われた．"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410282680026294658","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000005195379"}],"foaf:name":[{"@language":"ja","@value":"貞升 健志"},{"@language":"en","@value":"SADAMASU Kenji"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Microbiology, Tokyo Metropolitan Institute of Public Health"},{"@language":"ja","@value":"東京都健康安全研究センター微生物部"}]},{"@id":"https://cir.nii.ac.jp/crid/1420001326234734720","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"70291218"},{"@type":"NRID","@value":"1000070291218"},{"@type":"NRID","@value":"9000254675441"},{"@type":"NRID","@value":"9000254675861"},{"@type":"NRID","@value":"9000016265858"},{"@type":"NRID","@value":"9000256567837"},{"@type":"NRID","@value":"9000255868158"},{"@type":"NRID","@value":"9000259845173"},{"@type":"NRID","@value":"9000254676225"},{"@type":"NRID","@value":"9000254675042"},{"@type":"NRID","@value":"9000253285246"},{"@type":"NRID","@value":"9000254676362"},{"@type":"NRID","@value":"9000005172025"},{"@type":"NRID","@value":"9000008349185"},{"@type":"NRID","@value":"9000255868974"},{"@type":"NRID","@value":"9000254677753"},{"@type":"NRID","@value":"9000254678038"},{"@type":"NRID","@value":"9000254674858"},{"@type":"NRID","@value":"9000006138610"},{"@type":"NRID","@value":"9000254674878"},{"@type":"NRID","@value":"9000005632293"},{"@type":"NRID","@value":"9000254676876"},{"@type":"NRID","@value":"9000254212965"},{"@type":"NRID","@value":"9000254213150"},{"@type":"NRID","@value":"9000254212742"},{"@type":"NRID","@value":"9000254677687"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0159500"}],"foaf:name":[{"@language":"ja","@value":"小島 洋子"},{"@language":"en","@value":"KOJIMA Yoko"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Division of virology, Department of Infectious Diseases, Osaka Prefectural Institute of Public Health"},{"@language":"ja","@value":"大阪府立公衆衛生研究所感染症部ウイルス課"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658395358720","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"20250300"},{"@type":"NRID","@value":"1000020250300"},{"@type":"NRID","@value":"9000255865043"},{"@type":"NRID","@value":"9000256567836"},{"@type":"NRID","@value":"9000392647058"},{"@type":"NRID","@value":"9000255868160"},{"@type":"NRID","@value":"9000255868975"},{"@type":"NRID","@value":"9000004183579"},{"@type":"NRID","@value":"9000256562401"},{"@type":"NRID","@value":"9000255866885"},{"@type":"NRID","@value":"9000255865305"},{"@type":"NRID","@value":"9000255866834"},{"@type":"NRID","@value":"9000364938779"}],"foaf:name":[{"@language":"ja","@value":"森 治代"},{"@language":"en","@value":"MORI Haruyo"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Division of virology, Department of Infectious Diseases, Osaka Prefectural Institute of Public Health"},{"@language":"ja","@value":"大阪府立公衆衛生研究所感染症部ウイルス課"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801201430400","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80270768"},{"@type":"NRID","@value":"1000080270768"},{"@type":"NRID","@value":"9000254716121"},{"@type":"NRID","@value":"9000004168202"},{"@type":"NRID","@value":"9000254241777"},{"@type":"NRID","@value":"9000005161625"},{"@type":"NRID","@value":"9000020349433"},{"@type":"NRID","@value":"9000017388277"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/kawahata"}],"foaf:name":[{"@language":"ja","@value":"川畑 拓也"},{"@language":"en","@value":"KAWAHATA Takuya"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Division of virology, Department of Infectious Diseases, Osaka Prefectural Institute of Public Health"},{"@language":"ja","@value":"大阪府立公衆衛生研究所感染症部ウイルス課"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282680026294656","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000265564683"}],"foaf:name":[{"@language":"ja","@value":"長島 真美"},{"@language":"en","@value":"NAGASHIMA Mami"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Division of virology, Department of Microbiology, Tokyo Metropolitan Institute of Public Health"},{"@language":"ja","@value":"東京都健康安全研究センター微生物部ウイルス研究科"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"03875911"},{"@type":"LISSN","@value":"03875911"},{"@type":"EISSN","@value":"1884569X"},{"@type":"NDL_BIB_ID","@value":"000000004450"},{"@type":"ISSN","@value":"03875911"},{"@type":"NCID","@value":"AN00047715"}],"prism:publicationName":[{"@value":"感染症学雑誌"},{"@language":"ja","@value":"感染症学雑誌"},{"@language":"en","@value":"Kansenshogaku Zasshi"},{"@value":"感染症誌"},{"@language":"en","@value":"kansenshogakuzasshi"},{"@language":"ja","@value":"感染症誌"},{"@language":"en","@value":"J. J. A. Inf. D"}],"dc:publisher":[{"@language":"en","@value":"The Japanese Association for Infectious Diseases"},{"@language":"ja","@value":"一般社団法人 日本感染症学会"}],"prism:publicationDate":"2013","prism:volume":"87","prism:number":"4","prism:startingPage":"431","prism:endingPage":"434"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://id.ndl.go.jp/bib/024819888"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I024819888"},{"@id":"https://search.jamas.or.jp/link/ui/2013351723"}],"availableAt":"2013","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=HIV","dc:title":"HIV"},{"@id":"https://cir.nii.ac.jp/all?q=rapid%20diagnosis","dc:title":"rapid diagnosis"},{"@id":"https://cir.nii.ac.jp/all?q=acute%20HIV%20infection","dc:title":"acute HIV infection"},{"@id":"https://cir.nii.ac.jp/all?q=immunochromatography","dc:title":"immunochromatography"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782212929664","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"24590840"},{"@type":"JGN","@value":"JP24590840"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24590840/"}],"notation":[{"@language":"ja","@value":"ＨＩＶ感染者におけるＨＢＶ・梅毒トレポネーマの感染歴とＨＢＶ遺伝子型の解析"},{"@language":"en","@value":"Seroprevalence of HBV and Treponema pallidum in HIV-positive individuals and analysis of HBV genotype"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360574094029110400","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Evaluation of the Determine™ fourth generation HIV rapid assay"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855568911249152","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Quantitation of HIV-1 group M proviral DNA using TaqMan MGB real-time PCR"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699994790880128","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Evaluation of a rapid antigen and antibody combination test in acute HIV infection"}]},{"@id":"https://cir.nii.ac.jp/crid/1361975842821153664","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Acquired Removability of Aspartic Protease Inhibitors by Direct Biotinylation"}]},{"@id":"https://cir.nii.ac.jp/crid/1570291224347232384","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"HIV-IRNA定量法(KK-TaqMan法)国立感染症研究所病原体検出マニュアルエイズ"}]},{"@id":"https://cir.nii.ac.jp/crid/1570291224347234560","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Evaluation of the Determine fourth generation HIV rapid assay"}]},{"@id":"https://cir.nii.ac.jp/crid/1570572699323945344","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Evaluation of a rapid antigen and antibody combination test in acute HIV infection"}]},{"@id":"https://cir.nii.ac.jp/crid/1570572699323950336","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"新規迅速検査試薬の性能評価"}]},{"@id":"https://cir.nii.ac.jp/crid/1570854176159208192","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"HIV第4世代の迅速キット「エスプラインHIVAg/Ab」の性能評価"}]},{"@id":"https://cir.nii.ac.jp/crid/1571698599230790912","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Quantitation of HIV-1 group M proviral DNA using TaqMan MGB real-time PCR"}]},{"@id":"https://cir.nii.ac.jp/crid/1573105974114341888","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"ルミパルスPresto IIを用いたIIIVAg/Ab同時検出試薬の基礎性能評価"}]},{"@id":"https://cir.nii.ac.jp/crid/1573668925926771712","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"イムノクロマト法を原理とする「エスプラインHIVAg/Ab」の基礎的検討"}]}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:2000315186"},{"@type":"NDL_SEARCH","@value":"oai:ndlsearch.ndl.go.jp:R000000004-I024819888"},{"@type":"CROSSREF","@value":"10.11150/kansenshogakuzasshi.87.431"},{"@type":"CIA","@value":"10031201445"},{"@type":"KAKEN","@value":"PRODUCT-14695541"},{"@type":"OPENAIRE","@value":"doi_dedup___::7464c5ee44b552229294623dd2d3b4a0"},{"@type":"CROSSREF","@value":"10.1021/acs.bioconjchem.9b00195_references_DOI_4d5oonx8pyHND0982zvdcsyR9J8"}]}