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The results showed that tetramethylammonium, tetraethylammonium, tetra-<i>n</i>-propylammonium and tetra-<i>n</i>-butylammonium were degraded at different levels by anaerobic bacteria, but that tetra-<i>n</i>-pentylammonium and tetra-<i>n</i>-hexylammonium were difficult to degrade and had acute toxicity to biogas production at concentrations of over 50 mgC · <I>l</I><sup>-1</sup>. TAA showed increasing inhibitory activity to the metabolism of glucose with an increase in alkyl chain length under anaerobic condition. A complete bioactivity recovery of anaerobic sludge inhibited by five TAA (C<sub>1∼5</sub>) could be achieved in two months. Nevertheless, the toxicity of tetra-<i>n</i>-hexylammonium to anaerobic microorganisms was irreversible, which was considered a substance highly hazardous to anaerobic microorganisms, particularly, methanogens."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410853647247365379","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000014274505"}],"foaf:name":[{"@language":"en","@value":"WANG Xin-hai"},{"@language":"ja","@value":"王 新海"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of Science and Engineering, Yamaguchi 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