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6. Calorimetric Studies on Post-dormant Development(Papers presented at the Seminar, "The latest developments in biopreservation technigues using low temperature and low moisture conditions", July 23, 2007, Tokyo)

  • NAGANO Yatsuhisa
    Research Center for Molecular Thermodynamics, Graduate School of Science, Osaka University
  • KUWAYAMA Hidekazu
    Graduate School of Life and Environmental Sciences, University of Tsukuba

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Other Title
  • 6. 休眠から発生再開の熱測定(セミナー「低温と乾燥による生物保存技術への挑戦~マミーエンジニアリング」)
  • 休眠から発生再開の熱測定
  • キュウミン カラ ハッセイ サイカイ ノ ネツ ソクテイ

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Abstract

High-sensitive biological calorimetry for living organisms is described. A commercial isothermal calorimeter (LKB 2277-201) was modified to measure thermogenesis less than 0.1 μW. Baseline was stabilized to be ±10 nW over a week. Glass ampoule with a screw cap made high sensitive measurement possible in an hour after loading a sample. Calorimetric studies of post-dormant development of Artemia franciscana and spore germination of Dictyostelium discoideum Ax-2 are presented as examples.

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