{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282680071332736.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1272/jnms.79.394"}},{"identifier":{"@type":"COI","@value":"1:STN:280:DC%2BC3s3ntVWmtQ%3D%3D"}},{"identifier":{"@type":"PMID","@value":"23291836"}},{"identifier":{"@type":"URI","@value":"https://www.jstage.jst.go.jp/article/jnms/79/6/79_394/_pdf"}},{"identifier":{"@type":"NAID","@value":"130004057397"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/2013287869"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Serotype-independent Method of Recombinant Adeno-associated Virus (AAV) Vector Production and Purification"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"A variety of gene transfer strategies have been developed to treat inherited, degenerative, and acquired diseases. Among the different vector systems developed so far, recombinant adeno-associated viral (AAV) vectors have shown notable benefits, including prolonged gene expression, transduction of both dividing and nondividing cells, and a lack of pathogenicity caused by wild-type infections. Thanks to these features, the use of AAV vectors as a gene transfer tool has increased dramatically during the past several years, and several recent clinical trials have used AAV vectors. However, AAV vectors are more complicated to produce than are other viral vectors. With steady advances toward clinical application, much effort has been made to isolate novel AAV serotypes and to develop methods for their efficient, scalable, and versatile production and purification. Here we review state of the art methods for AAV vector production and purification, which we have refined in our laboratory.<br>"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420564276179237376","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"90267211"},{"@type":"NRID","@value":"1000090267211"},{"@type":"NRID","@value":"9000002921473"},{"@type":"NRID","@value":"9000258150388"},{"@type":"NRID","@value":"9000255696608"},{"@type":"NRID","@value":"9000253502739"},{"@type":"NRID","@value":"9000011199953"},{"@type":"NRID","@value":"9000256005003"},{"@type":"NRID","@value":"9000256003582"},{"@type":"NRID","@value":"9000412230640"},{"@type":"NRID","@value":"9000257926584"},{"@type":"NRID","@value":"9000014680297"},{"@type":"NRID","@value":"9000256005880"},{"@type":"NRID","@value":"9000350631846"},{"@type":"NRID","@value":"9000401693937"},{"@type":"NRID","@value":"9000000501859"},{"@type":"NRID","@value":"9000257926560"},{"@type":"NRID","@value":"9000005697121"},{"@type":"NRID","@value":"9000283533659"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/Koichi-Miyake5350"}],"foaf:name":[{"@language":"en","@value":"Miyake Koichi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Biochemistry and Molecular Biology, Division of Gene Therapy Research Center for Advanced Medical Technology, Nippon Medical School"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801192816640","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"00421206"},{"@type":"NRID","@value":"1000000421206"},{"@type":"NRID","@value":"9000256789293"},{"@type":"NRID","@value":"9000255696609"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/Nor7"}],"foaf:name":[{"@language":"en","@value":"Miyake Noriko"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Biochemistry and Molecular Biology, Division of Gene Therapy Research Center for Advanced Medical Technology, Nippon Medical School"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282680071332738","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000255696611"}],"foaf:name":[{"@language":"en","@value":"Shimada Takashi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Biochemistry and Molecular Biology, Division of Gene Therapy Research Center for Advanced Medical Technology, Nippon Medical School"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801191719808","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"90407685"},{"@type":"NRID","@value":"1000090407685"},{"@type":"NRID","@value":"9000255696610"},{"@type":"NRID","@value":"9000347064545"},{"@type":"NRID","@value":"9000347069534"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/yoshiyuki-yamazaki"}],"foaf:name":[{"@language":"en","@value":"Yamazaki Yoshiyuki"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Biochemistry and Molecular Biology, Division of Gene Therapy Research Center for Advanced Medical Technology, Nippon Medical School"}]},{"@id":"https://cir.nii.ac.jp/crid/1420845751158009344","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"10089617"},{"@type":"NRID","@value":"1000010089617"},{"@type":"NRID","@value":"9000003837599"},{"@type":"NRID","@value":"9000255696612"},{"@type":"NRID","@value":"9000413474269"},{"@type":"NRID","@value":"9000413475591"},{"@type":"NRID","@value":"9000398422016"},{"@type":"NRID","@value":"9000398422027"},{"@type":"NRID","@value":"9000022204048"},{"@type":"NRID","@value":"9000402617051"},{"@type":"NRID","@value":"9000253515594"},{"@type":"NRID","@value":"9000256003644"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/Yukihiko-HIRAI"}],"foaf:name":[{"@language":"en","@value":"Hirai Yukihiko"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Biochemistry and Molecular Biology, Division of Gene Therapy Research Center for Advanced Medical Technology, Nippon Medical School"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13454676"},{"@type":"EISSN","@value":"13473409"},{"@type":"CODEN","@value":"JNMSF5"}],"prism:publicationName":[{"@language":"en","@value":"Journal of Nippon Medical School"},{"@language":"en","@value":"J Nippon Med Sch"},{"@language":"ja","@value":"日医大誌"}],"dc:publisher":[{"@language":"en","@value":"The Medical Association of Nippon Medical School"},{"@language":"ja","@value":"日本医科大学医学会"}],"prism:publicationDate":"2012","prism:volume":"79","prism:number":"6","prism:startingPage":"394","prism:endingPage":"402"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"https://www.jstage.jst.go.jp/article/jnms/79/6/79_394/_pdf"},{"@id":"https://search.jamas.or.jp/link/ui/2013287869"}],"availableAt":"2012","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=AAV%20vector","dc:title":"AAV vector"},{"@id":"https://cir.nii.ac.jp/all?q=Serotype","dc:title":"Serotype"},{"@id":"https://cir.nii.ac.jp/all?q=Self-complementary%20AAV","dc:title":"Self-complementary AAV"},{"@id":"https://cir.nii.ac.jp/all?q=Purification","dc:title":"Purification"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050566774703013120","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"High-Level Expression of Alkaline Phosphatase by Adeno-Associated Virus Vector Ameliorates Pathological Bone Structure in a Hypophosphatasia Mouse Model"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002218145077120","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"miR‐15b mediates oxaliplatin‐induced chronic neuropathic pain through BACE1 down‐regulation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004231509026304","@type":"Article","resourceType":"学術雑誌論文(journal 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Vector–Mediated Gene Transfer in Hemophilia B"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855571489383296","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Transfection of mammalian cells using linear polyethylenimine is a simple and effective means of producing recombinant adeno-associated virus vectors"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137043626593920","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Large-scale recombinant adeno-associated virus production"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137046458096896","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Rapid and simplified purification of recombinant adeno-associated virus"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699994399849856","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Self-complementary recombinant adeno-associated virus (scAAV) vectors promote efficient transduction 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