{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282680173880448.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.11416/jibs.82.3_061"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"025556154"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/025556154"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I025556154"}},{"identifier":{"@type":"NAID","@value":"130004935607"}}],"dc:title":[{"@language":"en","@value":"Residue-specific incorporation of phenylalanine analogues into protein biosynthesis in silkworm cultured cells"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"Here we describe the residue-specific incorporation of <i>para</i>-substituted L-phenylalanine (Phe) analogues into protein biosynthesis in <i>Bombyx mori</i> cultured cells, BmN, which express <i>B. mori</i> phenylalanyl-tRNA synthetase (BmPheRS) mutants with relaxed amino acid specificity. Aminoacylation capabilities of BmPheRS mutants (Ala450 to Gly and Thr407 to Ala or Gly mutants in the α-subunit) were first investigated <i>in vitro</i> and then the incorporation of Phe analogues into a reporter protein, EGFP, was investigated using BmN cells expressing one of the above mutants. We also evaluated the effects of lowering the Phe concentration in culture media, which competes with Phe analogues in aminoacylation reactions inside cells, and found that lowering Phe concentration was effective in increasing the substitution rate from Phe to its analogues in EGFP. We conducted electrophoretic and mass spectrometric analyses of synthesized EGFP to clarify the incorporation of Phe analogues. We obtained direct mass spectrometric data demonstrating the incorporation of <i>p</i>-bromo- and <i>p</i>-cyano-L-phenylalanine and indirect electrophoretic data, implying the incorporation of <i>p</i>-azido- and <i>p</i>-iodo-L-phenylalanine into protein biosynthesis in BmN cells.<br>"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420282801199466624","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"60391562"},{"@type":"NRID","@value":"1000060391562"},{"@type":"NRID","@value":"9000000882231"},{"@type":"NRID","@value":"9000318139386"},{"@type":"NRID","@value":"9000007038366"},{"@type":"NRID","@value":"9000258009001"},{"@type":"NRID","@value":"9000402484245"},{"@type":"NRID","@value":"9000356629996"},{"@type":"NRID","@value":"9000258009006"},{"@type":"NRID","@value":"9000286634202"},{"@type":"NRID","@value":"9000283612188"},{"@type":"NRID","@value":"9000018471984"},{"@type":"NRID","@value":"9000345423545"},{"@type":"NRID","@value":"9000396166629"},{"@type":"NRID","@value":"9000283578894"},{"@type":"NRID","@value":"9000257992614"},{"@type":"NRID","@value":"9000332695181"},{"@type":"NRID","@value":"9000347078732"},{"@type":"NRID","@value":"9000243876594"},{"@type":"NRID","@value":"9000413384650"},{"@type":"NRID","@value":"9000288817436"},{"@type":"NRID","@value":"9000400163489"},{"@type":"NRID","@value":"9000409878680"},{"@type":"NRID","@value":"9000283612740"},{"@type":"NRID","@value":"9000391919416"},{"@type":"NRID","@value":"9000258009168"},{"@type":"NRID","@value":"9000375894041"},{"@type":"NRID","@value":"9000258009062"},{"@type":"NRID","@value":"9000283612257"},{"@type":"NRID","@value":"9000017676356"},{"@type":"NRID","@value":"9000403046270"},{"@type":"NRID","@value":"9000391919410"},{"@type":"NRID","@value":"9000283612778"},{"@type":"NRID","@value":"9000001181919"},{"@type":"NRID","@value":"9000391920542"},{"@type":"NRID","@value":"9000283612187"},{"@type":"NRID","@value":"9000286634159"},{"@type":"NRID","@value":"9000392068346"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0084985"}],"foaf:name":[{"@language":"en","@value":"Teramoto Hidetoshi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Silk Materials Research Unit, Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences (NIAS)"}]},{"@id":"https://cir.nii.ac.jp/crid/1420564276185042816","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"40370655"},{"@type":"NRID","@value":"1000040370655"},{"@type":"NRID","@value":"9000006334731"},{"@type":"NRID","@value":"9000391928264"},{"@type":"NRID","@value":"9000006900213"},{"@type":"NRID","@value":"9000329413517"},{"@type":"NRID","@value":"9000413567649"},{"@type":"NRID","@value":"9000399795367"},{"@type":"NRID","@value":"9000391933395"},{"@type":"NRID","@value":"9000345423546"},{"@type":"NRID","@value":"9000283578895"},{"@type":"NRID","@value":"9000332695182"},{"@type":"NRID","@value":"9000020877162"},{"@type":"NRID","@value":"9000375894779"},{"@type":"NRID","@value":"9000000965101"},{"@type":"NRID","@value":"9000391920264"},{"@type":"NRID","@value":"9000391835346"},{"@type":"NRID","@value":"9000413380210"},{"@type":"NRID","@value":"9000391917082"},{"@type":"NRID","@value":"9000391917652"},{"@type":"NRID","@value":"9000396166416"},{"@type":"NRID","@value":"9000391397021"},{"@type":"NRID","@value":"9000257895292"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0059874"}],"foaf:name":[{"@language":"en","@value":"Kojima Katsura"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Silk Materials Research Unit, Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences (NIAS)"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13468073"},{"@type":"LISSN","@value":"13468073"},{"@type":"EISSN","@value":"18847978"},{"@type":"NDL_BIB_ID","@value":"000000392750"},{"@type":"ISSN","@value":"13468073"},{"@type":"NCID","@value":"AA11558849"}],"prism:publicationName":[{"@language":"en","@value":"Journal of Insect Biotechnology and Sericology"},{"@language":"ja","@value":"Ｊｏｕｒｎａｌ　ｏｆ　Ｉｎｓｅｃｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ　ａｎｄ　Ｓｅｒｉｃｏｌｏｇｙ"},{"@language":"en","@value":"J. Insect Biotechnol. Sericol."},{"@language":"en","@value":"JIBS, J. Insect Biotechnol. Sericol."},{"@language":"ja","@value":"ＪＩＢＳ，　Ｊ．　Ｉｎｓｅｃｔ　Ｂｉｏｔｅｃｈｎｏｌ．　Ｓｅｒｉｃｏｌ．"}],"dc:publisher":[{"@language":"en","@value":"The Japanese Society of Sericultural Science"},{"@language":"ja","@value":"一般社団法人 日本蚕糸学会"}],"prism:publicationDate":"2013","prism:volume":"82","prism:number":"3","prism:startingPage":"3_061","prism:endingPage":"3_069"},"url":[{"@id":"http://id.ndl.go.jp/bib/025556154"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I025556154"}],"availableAt":"2013","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=unnatural%20amino%20acids","dc:title":"unnatural amino acids"},{"@id":"https://cir.nii.ac.jp/all?q=phenylalanine%20analogues","dc:title":"phenylalanine analogues"},{"@id":"https://cir.nii.ac.jp/all?q=aminoacyl-tRNA%20synsthetase","dc:title":"aminoacyl-tRNA synsthetase"},{"@id":"https://cir.nii.ac.jp/all?q=BmN%20cells","dc:title":"BmN cells"},{"@id":"https://cir.nii.ac.jp/all?q=silkworm","dc:title":"silkworm"},{"@id":"https://cir.nii.ac.jp/all?q=%3Ci%3EBombyx%20mori%3C/i%3E","dc:title":"<i>Bombyx mori</i>"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360302865743396992","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Unique Material Properties of <i>Bombyx mori</i> Silk Fiber Incorporated with 3-Azidotyrosine"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565166568534528","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Azide-Incorporated Clickable Silk Fibroin Materials with the Ability to Photopattern"}]}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:1003765424"},{"@type":"NDL_SEARCH","@value":"oai:ndlsearch.ndl.go.jp:R000000004-I025556154"},{"@type":"CIA","@value":"130004935607"},{"@type":"CROSSREF","@value":"10.1021/acs.biomac.3c00533_references_DOI_HYQowotGv3qusXBR21x5kPPooWp"},{"@type":"CROSSREF","@value":"10.1021/acsbiomaterials.5b00469_references_DOI_HYQowotGv3qusXBR21x5kPPooWp"}]}