{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282680192038528.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1269/jrr.39.263"}},{"identifier":{"@type":"COI","@value":"1:CAS:528:DyaK1MXhsFSjsbo%3D"}},{"identifier":{"@type":"PMID","@value":"10196781"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"4648884"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/4648884"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I4648884"}},{"identifier":{"@type":"URI","@value":"https://dl.ndl.go.jp/pid/10916170"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"10916170"}},{"identifier":{"@type":"NAID","@value":"110002342231"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/digimeta/10916170"}},{"identifier":{"@type":"URI","@value":"http://academic.oup.com/jrr/article-pdf/39/4/263/2737371/jrr-39-263.pdf"}},{"identifier":{"@type":"URI","@value":"http://dl.ndl.go.jp/info:ndljp/pid/10916170"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"An Improved System for Selection of Forward Mutations in an Escherichia coli supF Gene Carried by Plasmids."},{"@language":"ja-Kana","@value":"Improved System for Selection of Forward Mutations in an Escherichia coli supF Gene Carried by Plasmids"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"An improved system to examine forward mutations that occurred in the <i>supF</i> gene of <i>Escherichai coli</i> carried on a multicopy plasmid is described. The system was validated by measuring spontaneous mutations of <i>supF</i> plasmids propagated in wild-type, <i>recA</i><SUP>-</SUP> and <i>mutM</i><SUP>-</SUP> <i>mutY</i><SUP>-</SUP> <i>E. coli</i> strains, the mutation frequencies of which were 1.3 × 10<SUP>-7</SUP>, 6.3 × 10<SUP>-7</SUP> and 1.5 × 10<SUP>-6</SUP>, respectively. Sequence analysis of the <i>supF</i> mutant plasmids revealed that G:C→T:A and G:C→C:G transversions dominated. This improved system allows rapid scoring and sequencing forward mutations in the <i>supF</i> gene, thus permitting its use as a genetic target for repair and mutagenesis studies in bacteria and mammalian cells."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420845751154969216","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80333566"},{"@type":"NRID","@value":"1000080333566"},{"@type":"NRID","@value":"9000364815239"},{"@type":"NRID","@value":"9000005993188"},{"@type":"NRID","@value":"9000406386499"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/_fumikoobata"}],"foaf:name":[{"@language":"en","@value":"OBATA FUMIKO"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Biological Institute, Graduate School of Science, Tohoku 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TATSUO"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Biological Institute, Graduate School of Science, Tohoku University"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801192506496","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"00237942"},{"@type":"NRID","@value":"1000000237942"},{"@type":"NRID","@value":"9000021281027"},{"@type":"NRID","@value":"9000391636519"},{"@type":"NRID","@value":"9000258035407"},{"@type":"NRID","@value":"9000021450392"},{"@type":"NRID","@value":"9000330327881"},{"@type":"NRID","@value":"9000364815241"},{"@type":"NRID","@value":"9000008797926"},{"@type":"NRID","@value":"9000253163899"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0020465"}],"foaf:name":[{"@language":"en","@value":"HASHIMOTO-GOTOH TAMOTSU"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Biochemistry and Molecular Genetics, Kyoto Prefectural University of Medicine"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282680192038530","@type":"Researcher","foaf:name":[{"@language":"en","@value":"YAMAMOTO KAZUO"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Biological Institute, Graduate School of Science, Tohoku University"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"04493060"},{"@type":"EISSN","@value":"13499157"},{"@type":"NDL_BIB_ID","@value":"000000127791"},{"@type":"ISSN","@value":"04493060"},{"@type":"LISSN","@value":"04493060"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/04493060"},{"@type":"PISSN","@value":"https://id.crossref.org/issn/04493060"},{"@type":"NCID","@value":"AA00705792"}],"prism:publicationName":[{"@language":"en","@value":"Journal of Radiation Research"},{"@language":"en","@value":"JRR"}],"dc:publisher":[{"@language":"en","@value":"Journal of Radiation Research Editorial Committee"},{"@language":"ja","@value":"Journal of Radiation Research 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gene"},{"@id":"https://cir.nii.ac.jp/all?q=Spontaneous%20mutation","dc:title":"Spontaneous mutation"},{"@id":"https://cir.nii.ac.jp/all?q=Transversion","dc:title":"Transversion"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360013168790745216","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Paradoxical role of the major DNA repair protein, OGG1, in action-at-a-distance mutation induction by 8-oxo-7,8-dihydroguanine"}]},{"@id":"https://cir.nii.ac.jp/crid/1360013168793033600","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Action-at-a-distance mutations at 5′-GpA-3′ sites induced by oxidised guanine in WRN-knockdown cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360017279882293504","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Development of a 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