In vitro development of three-dimensional tissues is in high demand for regenerative medicine and drug screening. Here we present a novel inkjet head specialized for on-demand ejection of cell suspensions. Droplets are formed by membrane vibration, which is generated by a pulse actuation of the piezoelectric vibrator. During the non-ejecting period, the membrane is softly vibrated by a sinusoidal actuation to prevent the sedimentation of the cells inside the chamber.
We performed an initial feasibility study by ejecting a suspension of normal human dermal fibroblasts (NHDF) using this inkjet head. By combining the ejecting actuation and the stirring actuation, the number of cells per droplets remained stable for more than thirty minutes and cell viability was over 90%.
Next, we tried to construct three dimensional cardiac tissues by using cardiomyocytes derived from human iPS cells. Droplets with cardiomyocytes were ejected into the culture medium by repeating cycles of ejecting and stirring. After four days of incubation, the cells in the constructed cardiac tissue showed synchronous beating.
These results indicate that this inkjet head is suitable for the effective ejection of living cells.
"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410282680243762176","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000345304540"}],"foaf:name":[{"@language":"ja","@value":"中澤 聡"},{"@language":"en","@value":"Nakazawa Satoshi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Advanced Technology R&D Center, Ricoh Institute of Technology, Ricoh Corporation"},{"@language":"ja","@value":"株式会社リコー 先端技術研究センター"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282680243762179","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000345304542"}],"foaf:name":[{"@language":"ja","@value":"倉持 譲"},{"@language":"en","@value":"Kuramochi Yuzuru"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Advanced Technology R&D Center, Ricoh Institute of Technology, Ricoh Corporation"},{"@language":"ja","@value":"株式会社リコー 先端技術研究センター"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282680243762184","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000345304543"}],"foaf:name":[{"@language":"ja","@value":"松本 貴彦"},{"@language":"en","@value":"Matsumoto Takahiko"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Advanced Technology R&D Center, Ricoh Institute of Technology, Ricoh Corporation"},{"@language":"ja","@value":"株式会社リコー 先端技術研究センター"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001205270131200","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000345304541"}],"foaf:name":[{"@language":"ja","@value":"高木 大輔"},{"@language":"en","@value":"Takagi Daisuke"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Advanced Technology R&D Center, Ricoh Institute of Technology, Ricoh Corporation"},{"@language":"ja","@value":"株式会社リコー 先端技術研究センター"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282680243762177","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000345304544"}],"foaf:name":[{"@language":"ja","@value":"増子 龍也"},{"@language":"en","@value":"Mashiko Ryuuya"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Advanced Technology R&D Center, Ricoh Institute of Technology, Ricoh Corporation"},{"@language":"ja","@value":"株式会社リコー 先端技術研究センター"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282680243762180","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000345304545"}],"foaf:name":[{"@language":"ja","@value":"瀬尾 学"},{"@language":"en","@value":"Seo Manabu"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Institute of Future Technology, Ricoh Corporation"},{"@language":"ja","@value":"株式会社リコー 未来技術研究所"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282680243762182","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000345304546"}],"foaf:name":[{"@language":"ja","@value":"打方 佳郎"},{"@language":"en","@value":"Uchikata Yoshio"}],"jpcoar:affiliationName":[{"@language":"en","@value":"IJ Engine Development Center, Ricoh Corporation"},{"@language":"ja","@value":"株式会社リコー IJエンジン開発センター"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282680243762183","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000021806998"}],"foaf:name":[{"@language":"ja","@value":"松崎 典弥"},{"@language":"en","@value":"Matsusaki Michiya"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Applied Chemistry, Graduate School of Engineering, Osaka University"},{"@language":"ja","@value":"大阪大学大学院 工学研究科"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282680243762181","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000345220909"}],"foaf:name":[{"@language":"ja","@value":"明石 満"},{"@language":"en","@value":"Akashi Mitsuru"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of Frontier Biosciences, Osaka University"},{"@language":"ja","@value":"大阪大学大学院 生命機能研究科"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"1347443X"},{"@type":"EISSN","@value":"18814379"}],"prism:publicationName":[{"@language":"en","@value":"Transactions of Japanese Society for Medical and Biological Engineering"},{"@language":"ja","@value":"生体医工学"},{"@language":"en","@value":"Trans. jpn. Soc. Med. Bio. Eng"},{"@language":"en","@value":"Transactions of Japanese Society for Medical and Biological Engineering"},{"@language":"ja","@value":"生体医工学"},{"@language":"en","@value":"TJSBME"}],"dc:publisher":[{"@language":"en","@value":"Japanese Society for Medical and Biological Engineering"},{"@language":"ja","@value":"公益社団法人 日本生体医工学会"}],"prism:publicationDate":"2016","prism:volume":"54Annual","prism:number":"27AM-Abstract","prism:startingPage":"S158","prism:endingPage":"S158"},"availableAt":"2016","dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:2001577564"},{"@type":"CIA","@value":"130005285315"}]}