{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282680263153152.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.11229/hpbb.2.29"}},{"identifier":{"@type":"NAID","@value":"130004503887"}}],"dc:title":[{"@language":"ja","@value":"高圧力下で結晶成長させたグルコースイソメラーゼ結晶の常圧下でのX線結晶構造解析"},{"@language":"en","@value":"X-ray structure analysis of glucose isomerase molecules in crystals prepared under high pressure"}],"dc:language":"ja","description":[{"type":"abstract","notation":[{"@language":"en","@value":"Three-dimensional structures of glucose isomerase molecules in crystals prepared from supersaturated solutions in capillaries under 0.1 and 100 MPa were determined by X-ray crystallography under 0.1 MPa. With accurate temperature control, we obtained a few large crystals of good quality in the capillaries under 0.1 and 100 MPa. X-ray structures with high quality data sets were successfully obtained without removing solution around the crystals. We also determined a relation between resolution of molecular structure and size of the crystals surrounded by the solution in the capillaries. However, the r.m.s. deviation of least square fit of all atom positions of both glucose isomerase folds was about 0.05Å. This deviation was little. The distributions of water sites in both glucose isomerase structures were almost identical."},{"@language":"ja","@value":"キャピラリー中にグルコースイソメラーゼ(GI)の過飽和溶液を満たし、0.1 MPaと100 MPa下で制御をしながら育成した結晶を用いて、共に0.1 MPa下でX線結晶構造解析を行った。結晶化の制御においては、0.1 MPa、100 MPa下で共に温度をコントロールすることで、キャピラリー中の結晶の数を抑え、X線結晶構造解析が可能な良質で大きな結晶を作製することができた。得られた結晶を両方とも0.1 MPa下に取り出してＸ線結晶構造解析を行ったところ、キャピラリー中に溶液が入ったままで高分解能の構造解析が可能なことが確認できた。また、溶液共存下での結晶サイズと分解能の相関についても明らかにできた。しかし、構造解析の結果、0.1 MPaと100 MPa下で作製した結晶の間で分子構造や水和構造に明確な変化はなかった。2つのタンパク質分子を重ね合わせて、対応する原子間距離を求めてみると、最大で約1.37 Å、平均で約0.078 Åの位置の違いしかなかった。また、水和構造においては、観測された水分子の位置について、得られた結果の中で最大約10 ％の位置の違いがあったが、0.1 MPaと100MPa下で作製した結晶の間での有意差は認められなかった。"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410282680263153153","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000258055782"}],"foaf:name":[{"@language":"ja","@value":"塚本 雅之"},{"@language":"en","@value":"Tsukamoto Masayuki"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Chemical Science and Technology, The University of Tokushima"},{"@language":"ja","@value":"徳島大学大学院先端技術科学教育部環境創生工学専攻化学機能創生コース"}]},{"@id":"https://cir.nii.ac.jp/crid/1420001326233728640","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"60325248"},{"@type":"NRID","@value":"1000060325248"},{"@type":"NRID","@value":"9000002478706"},{"@type":"NRID","@value":"9000003234153"},{"@type":"NRID","@value":"9000398559929"},{"@type":"NRID","@value":"9000005915090"},{"@type":"NRID","@value":"9000018756107"},{"@type":"NRID","@value":"9000018473429"},{"@type":"NRID","@value":"9000020374708"},{"@type":"NRID","@value":"9000253316372"},{"@type":"NRID","@value":"9000107391251"},{"@type":"NRID","@value":"9000005717651"},{"@type":"NRID","@value":"9000258119782"},{"@type":"NRID","@value":"9000003040281"},{"@type":"NRID","@value":"9000327084114"},{"@type":"NRID","@value":"9000402036808"},{"@type":"NRID","@value":"9000283619798"},{"@type":"NRID","@value":"9000007569438"},{"@type":"NRID","@value":"9000020440019"},{"@type":"NRID","@value":"9000020465427"},{"@type":"NRID","@value":"9000239575283"},{"@type":"NRID","@value":"9000254439623"},{"@type":"NRID","@value":"9000402042898"},{"@type":"NRID","@value":"9000401966087"},{"@type":"NRID","@value":"9000006642893"},{"@type":"NRID","@value":"9000401666275"},{"@type":"NRID","@value":"9000401646188"},{"@type":"NRID","@value":"9000405757983"},{"@type":"NRID","@value":"9000364896553"},{"@type":"NRID","@value":"9000331909718"},{"@type":"NRID","@value":"9000252988504"},{"@type":"NRID","@value":"9000402012705"},{"@type":"NRID","@value":"9000402048110"},{"@type":"NRID","@value":"9000401743103"},{"@type":"NRID","@value":"9000020390984"},{"@type":"NRID","@value":"9000401702837"},{"@type":"NRID","@value":"9000283619949"},{"@type":"NRID","@value":"9000258055862"},{"@type":"NRID","@value":"9000258135297"},{"@type":"NRID","@value":"9000257754448"},{"@type":"NRID","@value":"9000350540373"},{"@type":"NRID","@value":"9000237912764"},{"@type":"NRID","@value":"9000020170906"},{"@type":"NRID","@value":"9000021837815"},{"@type":"NRID","@value":"9000409578898"},{"@type":"NRID","@value":"9000277503157"},{"@type":"NRID","@value":"9000253114857"},{"@type":"NRID","@value":"9000401951137"},{"@type":"NRID","@value":"9000402474529"},{"@type":"NRID","@value":"9000404539923"},{"@type":"NRID","@value":"9000399515380"},{"@type":"NRID","@value":"9000283619882"},{"@type":"NRID","@value":"9000363235119"},{"@type":"NRID","@value":"9000003447731"},{"@type":"NRID","@value":"9000004760200"},{"@type":"NRID","@value":"9000257186910"},{"@type":"NRID","@value":"9000020533948"},{"@type":"NRID","@value":"9000403321349"},{"@type":"NRID","@value":"9000398284112"},{"@type":"NRID","@value":"9000008137846"},{"@type":"NRID","@value":"9000242241688"},{"@type":"NRID","@value":"9000250185473"},{"@type":"NRID","@value":"9000243744248"},{"@type":"NRID","@value":"9000401978467"},{"@type":"NRID","@value":"9000283619792"},{"@type":"NRID","@value":"9000258055783"},{"@type":"NRID","@value":"9000241658816"},{"@type":"NRID","@value":"9000238246184"},{"@type":"NRID","@value":"9000002579773"},{"@type":"NRID","@value":"9000272245652"},{"@type":"NRID","@value":"9000243754207"},{"@type":"NRID","@value":"9000240120730"},{"@type":"NRID","@value":"9000020307665"},{"@type":"NRID","@value":"9000020537973"},{"@type":"NRID","@value":"9000408830872"},{"@type":"NRID","@value":"9000243743471"},{"@type":"NRID","@value":"9000370756031"},{"@type":"NRID","@value":"9000401738807"},{"@type":"NRID","@value":"9000409010761"},{"@type":"NRID","@value":"9000398988825"},{"@type":"NRID","@value":"9000258183332"},{"@type":"NRID","@value":"9000002203502"},{"@type":"NRID","@value":"9000267814346"},{"@type":"NRID","@value":"9000020414156"},{"@type":"NRID","@value":"9000020463063"},{"@type":"NRID","@value":"9000020537717"},{"@type":"NRID","@value":"9000408831275"},{"@type":"NRID","@value":"9000237751799"},{"@type":"NRID","@value":"9000277510944"},{"@type":"NRID","@value":"9000391813446"},{"@type":"NRID","@value":"9000365080306"},{"@type":"NRID","@value":"9000254439493"},{"@type":"NRID","@value":"9000020537995"},{"@type":"NRID","@value":"9000237751802"},{"@type":"NRID","@value":"9000287291877"},{"@type":"NRID","@value":"9000401638800"},{"@type":"NRID","@value":"9000283244149"},{"@type":"NRID","@value":"9000256358920"},{"@type":"NRID","@value":"9000322092266"},{"@type":"NRID","@value":"9000020410040"},{"@type":"NRID","@value":"9000020372647"},{"@type":"NRID","@value":"9000258055743"},{"@type":"NRID","@value":"9000258154802"},{"@type":"NRID","@value":"9000008312969"},{"@type":"NRID","@value":"9000001281406"},{"@type":"NRID","@value":"9000392736126"},{"@type":"NRID","@value":"9000331908975"},{"@type":"NRID","@value":"9000411000453"},{"@type":"NRID","@value":"9000243743465"},{"@type":"NRID","@value":"9000388525379"},{"@type":"NRID","@value":"9000401677219"},{"@type":"NRID","@value":"9000020538026"},{"@type":"NRID","@value":"9000411077414"},{"@type":"NRID","@value":"9000401745519"},{"@type":"RESEARCHMAP","@valu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春彦"},{"@language":"en","@value":"Sakuraba Haruhiko"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Applied Biological Science, Faculty of Agriculture, Kagawa University"},{"@language":"ja","@value":"香川大学農学部応用生物科学科"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282680263153154","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000258055785"}],"foaf:name":[{"@language":"ja","@value":"田村 勝弘"},{"@language":"en","@value":"Tamura Katsuhiro"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Life System Institute and Technology and Science, The University of Tokushima"},{"@language":"ja","@value":"徳島大学大学院ソシオテクノサイエンス研究部ライフシステム部門"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"18821723"}],"prism:publicationName":[{"@language":"en","@value":"High Pressure Bioscience and Biotechnology"},{"@language":"ja","@value":"高圧バイオサイエンスとバイオテクノロジー"},{"@language":"en","@value":"High Pressure Bioscience and Biotechnology"},{"@language":"ja","@value":"高圧バイオサイエンスとバイオテクノロジー"}],"dc:publisher":[{"@language":"en","@value":"Japanese Research Group of High Pressure Bioscience and Biotechnology"},{"@language":"ja","@value":"生物関連高圧研究会"}],"prism:publicationDate":"2008","prism:volume":"2","prism:number":"1","prism:startingPage":"29","prism:endingPage":"37"},"availableAt":"2008","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=%E3%82%AD%E3%83%A3%E3%83%94%E3%83%A9%E3%83%AA%E3%83%BC","dc:title":"キャピラリー"},{"@id":"https://cir.nii.ac.jp/all?q=X%E7%B7%9A%E7%B5%90%E6%99%B6%E6%A7%8B%E9%80%A0%E8%A7%A3%E6%9E%90","dc:title":"X線結晶構造解析"},{"@id":"https://cir.nii.ac.jp/all?q=%E7%B5%90%E6%99%B6%E5%8C%96%E5%88%B6%E5%BE%A1","dc:title":"結晶化制御"},{"@id":"https://cir.nii.ac.jp/all?q=%E4%B8%89%E6%AC%A1%E5%85%83%E5%88%86%E5%AD%90%E6%A7%8B%E9%80%A0","dc:title":"三次元分子構造"},{"@id":"https://cir.nii.ac.jp/all?q=%E6%B0%B4%E5%92%8C%E6%A7%8B%E9%80%A0","dc:title":"水和構造"},{"@id":"https://cir.nii.ac.jp/all?q=capillary","dc:title":"capillary"},{"@id":"https://cir.nii.ac.jp/all?q=X-ray%20crystallography","dc:title":"X-ray crystallography"},{"@id":"https://cir.nii.ac.jp/all?q=Three-dimensional%20structure","dc:title":"Three-dimensional structure"},{"@id":"https://cir.nii.ac.jp/all?q=hydration","dc:title":"hydration"},{"@id":"https://cir.nii.ac.jp/all?q=crystallization%20control","dc:title":"crystallization control"}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:0031865973"},{"@type":"CIA","@value":"130004503887"}]}