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VAN3, an Arabidopsis novel type ArfGAP protein, functions in a vesicle transport governing vascular continuity
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- Naramoto Satoshi
- Department of Biological Sciences, Graduate School of Science, University of Tokyo
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- Sawa Shinichiro
- Department of Biological Sciences, Graduate School of Science, University of Tokyo
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- Koizumi Koji
- Department of Biological Sciences, Graduate School of Science, University of Tokyo
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- Kurihara Ippei
- Department of Biological Sciences, Graduate School of Science, University of Tokyo
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- Kubo Minoru
- Plant Science Center, RIKEN
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- Demura Taku
- Plant Science Center, RIKEN
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- Yahara Natsuko
- Molecular Membrane Biology Laboratory , RIKEN
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- Ueda Takashi
- Molecular Membrane Biology Laboratory , RIKEN
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- Nakano Akihiko
- Department of Biological Sciences, Graduate School of Science, University of Tokyo Molecular Membrane Biology Laboratory , RIKEN
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- Fukuda Hiroo
- Department of Biological Sciences, Graduate School of Science, University of Tokyo Plant Science Center, RIKEN
Bibliographic Information
- Other Title
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- シロイヌナズナ <I>VAN3</I> 遺伝子は葉脈の連続性を構築する小胞輸送機構に働く
Description
We have cloned the VAN3 gene as a causal gene of van3 mutant, defective in leaf vascular continuity. VAN3 encodes a putative novel type ArfGAP protein in Arabidopsis thaliana. Here we report ArfGAP activity of VAN3, and its subcellular localization. <br> First, we determined ArfGAP activity of VAN3 by an in vitro GTP/GDP exchange assay. VAN3 protein, which was prepared from E.coli havoring VAN3, stimulated hydrolysis of GTP to GDP binding to yeast Arf1p. Next, we determined the subcellular localization of VAN3 using a fusion protein of VAN3 and Venus. VAN3-Venus expressed under the control of 35S promoter showed fluorescent dots moving around in the cytoplasm. Double labeling experiments using VAN3-Venus and FM4-64, revealed high colocalization of FM4-64 staining vesicles and VAN3-Venus staining dots, suggesting VAN3 locates in the endosomal compartment. Efforts are underway to reveal colocalization of GNOM ArfGEF and VAN3 ArfGAP, and cell specificity of VAN3 gene expression.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2004 (0), 548-548, 2004
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680605052288
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- NII Article ID
- 130006988679
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed