{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282680609532416.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.14841/jspp.2009.0.0752.0"}},{"identifier":{"@type":"NAID","@value":"130006994708"}}],"dc:title":[{"@language":"en","@value":"Efficient transcription terminater from <I>Arabidopsis</I>"},{"@language":"ja","@value":"転写終結領域が遺伝子発現に与える影響"}],"description":[{"type":"abstract","notation":[{"@language":"en","@value":"Most effort in expression vector development has been devoted to the identification and characterization of highly expressed and/or regulated promoter. Another important parameter of gene expression is 3'-processing and polyadenylation of pre-mRNA. Most eukaryotic mRNAs are cleaved post-transcriptionally at a specific sites downstream of polyadenylation signal in the 3'-untranslated region. Functional polyadenylation signals are required for efficient transcription termination. Furthermore, the strength of the poly(A) signal correlates with termination efficiency. Previous studies showed that different 3' end regions strongly affect the level of gene expression. We isolated the efficient transcription terminator contribute to express a foreign gene in plants more effectively. We compared the relative efficiencies of different 3' end regions from <I>Arabidopsis</I> genes. The 3' end region of the heat shock protein gene increases the mRNA level approximately 2-fold more than the nopaline synthase (NOS) terminator."},{"@language":"ja","@value":"転写終結領域（ターミネーター）は遺伝子の3’末端領域に位置し、転写終結およびmRNAの3’末端修飾（pre-mRNAの切断、poly(A)付加）を行う。この3’末端修飾を担うシスエレメントの変異はリードスルーを引き起こし、逆に効率の良い3’末端修飾は効率良く転写を終結させる。このため効率の悪いターミネーターは自身の発現量の低下に加えて、下流遺伝子の発現抑制やサイレンシングのトリガーとなる可能性が考えられる。<br>  我々は効率の良いターミネーターを取得するため、様々な発現様式を示す遺伝子の3’末端領域を解析した。シロイヌナズナ、タバコおよびイネを用いた一過性発現実験において、解析したほとんどのターミネーターは対照としたNOSターミネーターと同程度であった。ところが<I>heat shock protein</I> (<I>HSP</I> ) 遺伝子のターミネーターはシロイヌナズナ、タバコでは2～3倍、イネでは発現を5倍増加させた。この発現の増加はmRNA量の増加と相関していた。またシロイヌナズナシングルコピー形質転換体を用いた解析においても、<I>HSP</I>ターミネーターはNOSターミネーターの2～3倍のGUS活性および<I>GUS</I> mRNA量の増加を示した。<I>HSP</I>ターミネーターと翻訳エンハンサー（<I>NtADH</I> 5’-UTR）を組み合わせることにより、GUSタンパク質は全可溶性タンパク質の1％程度まで高蓄積した。"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410572171066259201","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000391956039"}],"foaf:name":[{"@language":"en","@value":"Nagaya Shingo"},{"@language":"ja","@value":"長屋 進吾"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of Biological Sciences, Nara Institute of Science and Technology"},{"@language":"ja","@value":"奈良先端大・バイオ"}]},{"@id":"https://cir.nii.ac.jp/crid/1410572171066259200","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000391956040"}],"foaf:name":[{"@language":"en","@value":"Mikawa Syuhei"},{"@language":"ja","@value":"三河 周平"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"奈良先端大・バイオ"},{"@language":"en","@value":"Graduate School of Biological Sciences, Nara Institute of Science and Technology"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658414726019","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"30029235"},{"@type":"NRID","@value":"1000030029235"},{"@type":"NRID","@value":"9000021026578"},{"@type":"NRID","@value":"9000363319797"},{"@type":"NRID","@value":"9000363289375"},{"@type":"NRID","@value":"9000363288257"},{"@type":"NRID","@value":"9000363288818"},{"@type":"NRID","@value":"9000363288815"},{"@type":"NRID","@value":"9000006072394"},{"@type":"NRID","@value":"9000363292816"},{"@type":"NRID","@value":"9000363291003"},{"@type":"NRID","@value":"9000021512260"},{"@type":"NRID","@value":"9000391941470"},{"@type":"NRID","@value":"9000391925217"},{"@type":"NRID","@value":"9000401959122"},{"@type":"NRID","@value":"9000363300780"},{"@type":"NRID","@value":"9000363289546"},{"@type":"NRID","@value":"9000004228434"},{"@type":"NRID","@value":"9000363291244"},{"@type":"NRID","@value":"9000363292648"},{"@type":"NRID","@value":"9000363291008"},{"@type":"NRID","@value":"9000391930597"},{"@type":"NRID","@value":"9000391929902"},{"@type":"NRID","@value":"9000363289371"},{"@type":"NRID","@value":"9000363288913"},{"@type":"NRID","@value":"9000363288911"},{"@type":"NRID","@value":"9000363286481"},{"@type":"NRID","@value":"9000363290187"},{"@type":"NRID","@value":"9000014407094"},{"@type":"NRID","@value":"9000363292446"},{"@type":"NRID","@value":"9000021147371"},{"@type":"NRID","@value":"9000391933596"},{"@type":"NRID","@value":"9000391949755"},{"@type":"NRID","@value":"9000401962790"},{"@type":"NRID","@value":"9000363287391"},{"@type":"NRID","@value":"9000363290183"},{"@type":"NRID","@value":"9000363292650"},{"@type":"NRID","@value":"9000363290472"},{"@type":"NRID","@value":"9000363291006"},{"@type":"NRID","@value":"9000363291009"},{"@type":"NRID","@value":"9000239252997"},{"@type":"NRID","@value":"9000242100943"},{"@type":"NRID","@value":"9000401545646"},{"@type":"NRID","@value":"9000024948578"},{"@type":"NRID","@value":"9000363287874"},{"@type":"NRID","@value":"9000363286978"},{"@type":"NRID","@value":"9000363289372"},{"@type":"NRID","@value":"9000363288915"},{"@type":"NRID","@value":"9000363290292"},{"@type":"NRID","@value":"9000363292652"},{"@type":"NRID","@value":"9000363292355"},{"@type":"NRID","@value":"9000401542168"},{"@type":"NRID","@value":"9000401961734"},{"@type":"NRID","@value":"9000363287375"},{"@type":"NRID","@value":"9000363286771"},{"@type":"NRID","@value":"9000363287893"},{"@type":"NRID","@value":"9000363287465"},{"@type":"NRID","@value":"9000363289586"},{"@type":"NRID","@value":"9000363291619"},{"@type":"NRID","@value":"9000391940228"},{"@type":"NRID","@value":"9000391947755"},{"@type":"NRID","@value":"9000391928492"},{"@type":"NRID","@value":"9000391918889"},{"@type":"NRID","@value":"9000006163927"},{"@type":"NRID","@value":"9000363287219"},{"@type":"NRID","@value":"9000363287875"},{"@type":"NRID","@value":"9000363286972"},{"@type":"NRID","@value":"9000363288914"},{"@type":"NRID","@value":"9000363276410"},{"@type":"NRID","@value":"9000363291625"},{"@type":"NRID","@value":"9000363291534"},{"@type":"NRID","@value":"9000006338993"},{"@type":"NRID","@value":"9000363292349"},{"@type":"NRID","@value":"9000363293579"},{"@type":"NRID","@value":"9000363293538"},{"@type":"NRID","@value":"9000363293846"},{"@type":"NRID","@value":"9000391933637"},{"@type":"NRID","@value":"9000391924222"},{"@type":"NRID","@value":"9000391922599"},{"@type":"NRID","@value":"9000264861109"},{"@type":"NRID","@value":"9000363286497"},{"@type":"NRID","@value":"9000363288670"},{"@type":"NRID","@value":"9000363290185"},{"@type":"NRID","@value":"9000363291536"},{"@type":"NRID","@value":"9000363292353"},{"@type":"NRID","@value":"9000391926839"},{"@type":"NRID","@value":"9000391922956"},{"@type":"NRID","@value":"9000004120702"},{"@type":"NRID","@value":"9000363291626"},{"@type":"NRID","@value":"9000363292555"},{"@type":"NRID","@value":"9000363293389"},{"@type":"NRID","@value":"9000363293305"},{"@type":"NRID","@value":"9000391929437"},{"@type":"NRID","@value":"9000391921685"},{"@type":"NRID","@value":"9000401962728"},{"@type":"NRID","@value":"9000363279045"},{"@type":"NRID","@value":"9000363287876"},{"@type":"NRID","@value":"9000363286974"},{"@type":"NRID","@value":"9000363293957"},{"@type":"NRID","@value":"9000391933631"},{"@type":"NRID","@value":"9000391941151"},{"@type":"NRID","@value":"9000391922662"},{"@type":"NRID","@value":"9000391919898"},{"@type":"NRID","@value":"9000401962750"},{"@type":"NRID","@value":"9000258617227"},{"@type":"NRID","@value":"9000363286775"},{"@type":"NRID","@value":"9000363287873"},{"@type":"NRID","@value":"9000363286973"},{"@type":"NRID","@value":"9000363289373"},{"@type":"NRID","@value":"9000363287586"},{"@type":"NRID","@value":"9000364983310"},{"@type":"NRID","@value":"9000363293536"},{"@type":"NRID","@value":"9000363293090"},{"@type":"NRID","@value":"9000391956041"},{"@type":"NRID","@value":"9000391935226"},{"@type":"NRID","@value":"9000391957757"},{"@type":"NRID","@value":"9000391925190"},{"@type":"NRID","@value":"9000401546049"},{"@type":"NRID","@value":"9000363287210"},{"@type":"NRID","@value":"9000363286990"},{"@type":"NRID","@value":"9000363288791"},{"@type":"NRID","@value":"9000363288668"},{"@type":"NRID","@value":"9000363287585"},{"@type":"NRID","@value":"9000363290294"},{"@type":"NRID","@value":"9000363291620"},{"@type":"NRID","@value":"9000363291621"},{"@type":"NRID","@value":"9000363291702"},{"@type":"NRID","@value":"9000363291538"},{"@type":"NRID","@value":"9000363290997"},{"@type":"NRID","@value":"9000363285669"},{"@type":"NRID","@value":"9000363284263"},{"@type":"NRID","@value":"9000391921682"},{"@type":"NRID","@value":"9000401961510"},{"@type":"NRID","@value":"9000363286770"},{"@type":"NRID","@value":"9000363275361"},{"@type":"NRID","@value":"9000363284181"},{"@type":"NRID","@value":"9000363286989"},{"@type":"NRID","@value":"9000363290184"},{"@type":"NRID","@value":"9000363290189"},{"@type":"NRID","@value":"9000364987340"},{"@type":"NRID","@value":"9000364991761"},{"@type":"NRID","@value":"9000363292649"},{"@type":"NRID","@value":"9000363292351"},{"@type":"NRID","@value":"9000363291005"},{"@type":"NRID","@value":"9000391953100"},{"@type":"NRID","@value":"9000391949320"},{"@type":"NRID","@value":"9000391922557"},{"@type":"NRID","@value":"9000401544405"},{"@type":"NRID","@value":"9000401544754"},{"@type":"NRID","@value":"9000363289541"},{"@type":"NRID","@value":"9000363290144"},{"@type":"NRID","@value":"9000363290145"},{"@type":"NRID","@value":"9000363288912"},{"@type":"NRID","@value":"9000363288817"},{"@type":"NRID","@value":"9000363288667"},{"@type":"NRID","@value":"9000363290293"},{"@type":"NRID","@value":"9000363291623"},{"@type":"NRID","@value":"9000363293391"},{"@type":"NRID","@value":"9000363292347"},{"@type":"NRID","@value":"9000363292357"},{"@type":"NRID","@value":"9000363290615"},{"@type":"NRID","@value":"9000391926564"},{"@type":"NRID","@value":"9000363287376"},{"@type":"NRID","@value":"9000363277428"},{"@type":"NRID","@value":"9000363293865"},{"@type":"NRID","@value":"9000363293387"},{"@type":"NRID","@value":"9000391933599"},{"@type":"NRID","@value":"9000391918270"},{"@type":"NRID","@value":"9000391926378"},{"@type":"NRID","@value":"9000391921693"},{"@type":"NRID","@value":"9000401545932"},{"@type":"NRID","@value":"9000309177828"},{"@type":"NRID","@value":"9000363278669"},{"@type":"NRID","@value":"9000363279304"},{"@type":"NRID","@value":"9000363279563"},{"@type":"NRID","@value":"9000364967255"},{"@type":"NRID","@value":"9000364971795"},{"@type":"NRID","@value":"9000363276695"},{"@type":"NRID","@value":"9000363277435"},{"@type":"NRID","@value":"9000363276806"},{"@type":"NRID","@value":"9000363277155"},{"@type":"NRID","@value":"9000363280253"},{"@type":"NRID","@value":"9000363274738"},{"@type":"NRID","@value":"9000364970727"},{"@type":"NRID","@value":"9000364971874"},{"@type":"NRID","@value":"9000363275897"}],"foaf:name":[{"@language":"en","@value":"Shinmyo Atsuhiko"},{"@language":"ja","@value":"新名 惇彦"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of Biological Sciences, Nara Institute of Science and Technology"},{"@language":"ja","@value":"奈良先端大・バイオ"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801190334208","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80283935"},{"@type":"NRID","@value":"1000080283935"},{"@type":"NRID","@value":"9000004410531"},{"@type":"NRID","@value":"9000394467388"},{"@type":"NRID","@value":"9000006072392"},{"@type":"NRID","@value":"9000021512253"},{"@type":"NRID","@value":"9000391958230"},{"@type":"NRID","@value":"9000391941471"},{"@type":"NRID","@value":"9000391949109"},{"@type":"NRID","@value":"9000391930590"},{"@type":"NRID","@value":"9000391925218"},{"@type":"NRID","@value":"9000243879567"},{"@type":"NRID","@value":"9000010214508"},{"@type":"NRID","@value":"9000258724086"},{"@type":"NRID","@value":"9000391968233"},{"@type":"NRID","@value":"9000391930593"},{"@type":"NRID","@value":"9000391929903"},{"@type":"NRID","@value":"9000401957948"},{"@type":"NRID","@value":"9000005115336"},{"@type":"NRID","@value":"9000275823184"},{"@type":"NRID","@value":"9000107387090"},{"@type":"NRID","@value":"9000242898856"},{"@type":"NRID","@value":"9000401544400"},{"@type":"NRID","@value":"9000399777735"},{"@type":"NRID","@value":"9000018146872"},{"@type":"NRID","@value":"9000025005560"},{"@type":"NRID","@value":"9000009201002"},{"@type":"NRID","@value":"9000006938870"},{"@type":"NRID","@value":"9000021217474"},{"@type":"NRID","@value":"9000274309531"},{"@type":"NRID","@value":"9000257999168"},{"@type":"NRID","@value":"9000007617129"},{"@type":"NRID","@value":"9000238238645"},{"@type":"NRID","@value":"9000003996971"},{"@type":"NRID","@value":"9000240042308"},{"@type":"NRID","@value":"9000401961730"},{"@type":"NRID","@value":"9000381069013"},{"@type":"NRID","@value":"9000004498895"},{"@type":"NRID","@value":"9000391940229"},{"@type":"NRID","@value":"9000391947756"},{"@type":"NRID","@value":"9000391918890"},{"@type":"NRID","@value":"9000243894767"},{"@type":"NRID","@value":"9000401956373"},{"@type":"NRID","@value":"9000401958021"},{"@type":"NRID","@value":"9000010412152"},{"@type":"NRID","@value":"9000403995712"},{"@type":"NRID","@value":"9000006338991"},{"@type":"NRID","@value":"9000291275002"},{"@type":"NRID","@value":"9000391933635"},{"@type":"NRID","@value":"9000391924220"},{"@type":"NRID","@value":"9000391922600"},{"@type":"NRID","@value":"9000401961505"},{"@type":"NRID","@value":"9000401957937"},{"@type":"NRID","@value":"9000001771453"},{"@type":"NRID","@value":"9000363273758"},{"@type":"NRID","@value":"9000299861130"},{"@type":"NRID","@value":"9000391974010"},{"@type":"NRID","@value":"9000391926838"},{"@type":"NRID","@value":"9000253091574"},{"@type":"NRID","@value":"9000000221062"},{"@type":"NRID","@value":"9000004120700"},{"@type":"NRID","@value":"9000242906215"},{"@type":"NRID","@value":"9000401957415"},{"@type":"NRID","@value":"9000018661060"},{"@type":"NRID","@value":"9000391941152"},{"@type":"NRID","@value":"9000391922663"},{"@type":"NRID","@value":"9000391919899"},{"@type":"NRID","@value":"9000401962749"},{"@type":"NRID","@value":"9000345450400"},{"@type":"NRID","@value":"9000002146617"},{"@type":"NRID","@value":"9000402130119"},{"@type":"NRID","@value":"9000391959343"},{"@type":"NRID","@value":"9000391956042"},{"@type":"NRID","@value":"9000391957758"},{"@type":"NRID","@value":"9000391925191"},{"@type":"NRID","@value":"9000266898853"},{"@type":"NRID","@value":"9000258740264"},{"@type":"NRID","@value":"9000019938670"},{"@type":"NRID","@value":"9000391966049"},{"@type":"NRID","@value":"9000391921680"},{"@type":"NRID","@value":"9000401961509"},{"@type":"NRID","@value":"9000401956295"},{"@type":"NRID","@value":"9000363253485"},{"@type":"NRID","@value":"9000002318836"},{"@type":"NRID","@value":"9000401956789"},{"@type":"NRID","@value":"9000008298706"},{"@type":"NRID","@value":"9000273388629"},{"@type":"NRID","@value":"9000391922558"},{"@type":"NRID","@value":"9000391923199"},{"@type":"NRID","@value":"9000401544404"},{"@type":"NRID","@value":"9000401544750"},{"@type":"NRID","@value":"9000019185458"},{"@type":"NRID","@value":"9000021959915"},{"@type":"NRID","@value":"9000312722541"},{"@type":"NRID","@value":"9000396127911"},{"@type":"NRID","@value":"9000396144558"},{"@type":"NRID","@value":"9000005412972"},{"@type":"NRID","@value":"9000021363608"},{"@type":"NRID","@value":"9000391918271"},{"@type":"NRID","@value":"9000391926377"},{"@type":"NRID","@value":"9000387906384"},{"@type":"NRID","@value":"9000401545931"},{"@type":"NRID","@value":"9000256053266"},{"@type":"NRID","@value":"9000019031388"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/ko-kato"}],"foaf:name":[{"@language":"en","@value":"Kato Ko"},{"@language":"ja","@value":"加藤 晃"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of Biological Sciences, Nara Institute of Science and Technology"},{"@language":"ja","@value":"奈良先端大・バイオ"}]}],"publication":{"prism:publicationName":[{"@language":"en","@value":"Plant and Cell Physiology Supplement"},{"@language":"ja","@value":"日本植物生理学会年会およびシンポジウム　講演要旨集"},{"@language":"en","@value":"Supplement"},{"@language":"ja","@value":"年会講演要旨集"}],"dc:publisher":[{"@language":"en","@value":"The Japanese Society of Plant Physiologists"},{"@language":"ja","@value":"日本植物生理学会"}],"prism:publicationDate":"2009","prism:volume":"2009","prism:number":"0","prism:startingPage":"0752","prism:endingPage":"0752"},"availableAt":"2009","dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:1003217285"},{"@type":"CIA","@value":"130006994708"}]}