Heparin, which is used as an anticoagulant for blood gas analysis, interferes with the measurement of ionized calcium (Ca2+). The IFCC guidelines recommend that the concentration of lithium heparin should not exceed 15 IU/mL and calcium-titrated heparin should be less than 50 IU/mL. The purpose of this study was to evaluate the effect of the following factors in comparison with nonheparinized blood (NH): type of heparin, heparin concentration, and the passage of time until measurement. We measured the Ca2+ concentration in venous blood collected from 10 healthy volunteers by using two types of blood gas syringes: low-concentration lithium heparin syringe (LH: Terumo) and calcium-titrated heparin syringe (BH: Becton Dickinson and Company). The Ca2+ concentration in LH decreased by about the lower clinically allowable limit (−0.05 mmol/L) at all the heparin concentrations (2.8, 4.7, and 14.0 IU/mL), and also decreased depending on the heparin concentration with the passage of time. The Ca2+ concentration in BH decreased by about the clinically allowable limit at 160 IU/mL heparin, which greatly exceeded the recommended heparin concentration. Our results indicate that BH is suitable for Ca2+ measurement because Ca2+ concentration was less affected in BH than in LH. In order to minimize the effect of heparin and the passage of time on Ca2+ measurement, it is essential that we should draw the recommended amount of blood to obtain the recommended heparin concentration, and measure immediately after collection, especially when using LH.
"},{"@language":"ja","@value":"血液ガス分析で抗凝固剤として使用されているヘパリンはイオン化カルシウム（Ca2+）の測定に影響を与えることが知られている。IFCCのガイドラインでは，ヘパリン濃度を低濃度ヘパリン15 IU/mL以下，カルシウム調整済みヘパリン50 IU/mL以下での測定が推奨されている。今回，健常職員10名の静脈血を対象とし，低濃度ヘパリン（LH：テルモ），カルシウム調整済みヘパリン（BH：日本ベクトン・ディッキンソン）の2種類の血液ガス分析用採血キットを用いて，抗凝固剤を含まない血液を対照としてCa2+測定におけるヘパリンの種類や測定時のヘパリン濃度，また測定までの時間経過による影響を検討した。LHは今回検討したヘパリン濃度（2.8, 4.7, 14.0 IU/mL）のいずれでも臨床的許容範囲下限（−0.05 mmol/L）程度の低下を認め，また時間経過によってヘパリン濃度に依存した低下を認めた。BHは推奨ヘパリン濃度を大きく上回る濃度（160.0 IU/mL）で臨床的許容範囲を超える低下を認めた。今回の検討結果より，BHはLHと比べCa2+の低下は軽度であったため，Ca2+測定時はBHの使用が有用であると考えられた。ヘパリン濃度および時間経過によるCa2+値への影響を最小限にするためには，推奨採血量を採取し推奨ヘパリン濃度で，加えて特にLH使用時は採取後速やかに測定することが重要である。
"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410282680719853826","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000345296727"}],"foaf:name":[{"@language":"ja","@value":"持田 志穂"},{"@language":"en","@value":"MOCHIDA Shiho"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Clinical Laboratory, St. Marianna University School of Medicine"},{"@language":"ja","@value":"聖マリアンナ医科大学病院臨床検査部"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282680719853824","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000345296728"}],"foaf:name":[{"@language":"ja","@value":"齋藤 裕美"},{"@language":"en","@value":"SAITO Hiromi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Clinical Laboratory, St. Marianna University School of Medicine"},{"@language":"ja","@value":"聖マリアンナ医科大学病院臨床検査部"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282680719853825","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000345296729"}],"foaf:name":[{"@language":"ja","@value":"赤津 哲"},{"@language":"en","@value":"AKATSU Satoshi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Clinical Laboratory, St. Marianna University School of Medicine"},{"@language":"ja","@value":"聖マリアンナ医科大学病院臨床検査部"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282680719853827","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000345296730"}],"foaf:name":[{"@language":"ja","@value":"山﨑 哲"},{"@language":"en","@value":"YAMAZAKI Satoshi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Clinical Laboratory, St. Marianna University School of Medicine"},{"@language":"ja","@value":"聖マリアンナ医科大学病院臨床検査部"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09158669"},{"@type":"EISSN","@value":"21885346"}],"prism:publicationName":[{"@language":"en","@value":"Japanese Journal of Medical Technology"},{"@language":"ja","@value":"医学検査"},{"@language":"en","@value":"Japanese Journal of Medical Technology"},{"@language":"ja","@value":"医学検査"}],"dc:publisher":[{"@language":"en","@value":"Japanese Association of Medical Technologists"},{"@language":"ja","@value":"一般社団法人 日本臨床衛生検査技師会"}],"prism:publicationDate":"2016","prism:volume":"65","prism:number":"5","prism:startingPage":"526","prism:endingPage":"532"},"url":[{"@id":"https://search.jamas.or.jp/link/ui/2017026801"}],"availableAt":"2016","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=%E8%A1%80%E6%B6%B2%E3%82%AC%E3%82%B9%E5%88%86%E6%9E%90","dc:title":"血液ガス分析"},{"@id":"https://cir.nii.ac.jp/all?q=%E3%82%A4%E3%82%AA%E3%83%B3%E5%8C%96%E3%82%AB%E3%83%AB%E3%82%B7%E3%82%A6%E3%83%A0","dc:title":"イオン化カルシウム"},{"@id":"https://cir.nii.ac.jp/all?q=%E3%83%98%E3%83%91%E3%83%AA%E3%83%B3%E6%BF%83%E5%BA%A6","dc:title":"ヘパリン濃度"},{"@id":"https://cir.nii.ac.jp/all?q=%E4%BD%8E%E6%BF%83%E5%BA%A6%E3%83%98%E3%83%91%E3%83%AA%E3%83%B3","dc:title":"低濃度ヘパリン"},{"@id":"https://cir.nii.ac.jp/all?q=%E3%82%AB%E3%83%AB%E3%82%B7%E3%82%A6%E3%83%A0%E8%AA%BF%E6%95%B4%E6%B8%88%E3%81%BF%E3%83%98%E3%83%91%E3%83%AA%E3%83%B3","dc:title":"カルシウム調整済みヘパリン"},{"@id":"https://cir.nii.ac.jp/all?q=blood%20gas%20analysis","dc:title":"blood gas analysis"},{"@id":"https://cir.nii.ac.jp/all?q=ionized%20calcium","dc:title":"ionized calcium"},{"@id":"https://cir.nii.ac.jp/all?q=heparin%20concentration","dc:title":"heparin concentration"},{"@id":"https://cir.nii.ac.jp/all?q=low%20concentration%20heparin","dc:title":"low concentration heparin"},{"@id":"https://cir.nii.ac.jp/all?q=calcium-titrated%20heparin","dc:title":"calcium-titrated heparin"}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:2001568585"},{"@type":"CIA","@value":"130005280188"}]}