{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282681105215744.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1248/yakushi.130.1207"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"10807207"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/10807207"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I10807207"}},{"identifier":{"@type":"URI","@value":"https://dl.ndl.go.jp/pid/10203935"}},{"identifier":{"@type":"URI","@value":"https://www.jstage.jst.go.jp/article/yakushi/130/9/130_9_1207/_pdf"}},{"identifier":{"@type":"NAID","@value":"130000342381"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/2010341127"}}],"dc:title":[{"@language":"en","@value":"Establishment of a Novel Model for Studying the Effects of Extracts of Chinese Herb Medicine on Human Type II 5α-Reductase <i>in Vitro</i>"},{"@value":"Establishment of a novel model for studying the effects of extracts of Chinese herb medicine on human type 2 5α-reductase in vitro"},{"@language":"ja-Kana","@value":"Establishment of a novel model for studying the effects of extracts of Chinese herb medicine on human type 2 5 a reductase in vitro"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"Human steroid 5α-reductase type II (hSRD5A2) and dihydrotestosterone (DHT) play important roles in benign prostatic hyperplasia (BPH). The aim of our study was to establish a novel model to investigate the inhibitory effects of extracts and compounds of Chinese herb medicine on hSRD5A2. The gene, hSRD5A2, was artificially synthesized and cloned into pcDNA3.1(+) vector, which was transfected into CHO cells by liposome. Transfected cells were screened through G418 and MTX. The expressed protein of hSRD5A2 by cells was purified and detected by western blotting. A minim reactive system comprising hSRD5A2 and testosterone (T) as substrate together with NADPH as hydrogen donor was established for screening inhibitors of hSRD5A2. The reaction system was optimized in the concentrations of T, NADPH, and hSRD5A2 and reaction temperature, time, and activity of hSRD5A2 were determined by the production of DHT. Furthermore, we screened some extracts and compounds of Chinese herb medicine using this model. The concentrations of T, NADPH, and hSRD5A2 were 0.02 μM, 0.8 mM, and 0.05 U/μl, respectively, in the model; maximum activity of hSRD5A2 was achieved at 37°C and 60 min reaction, and mangiferin had significant inhibitory effect on the activity of hSRD5A2. The model in this study is convenient and reliable for screening and evaluation of inhibitors of hSRD5A2; mangiferin may be a potential medicine for the treatment of BPH.<br>"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410282681105215748","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000020707656"}],"foaf:name":[{"@language":"en","@value":"WANG Xingsheng"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Pharmacy, Anhui University of Traditional Chinese Medicine"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282681105215747","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000020707663"}],"foaf:name":[{"@language":"en","@value":"LIAO Jianmin"}],"jpcoar:affiliationName":[{"@language":"en","@value":"School of Life Science and Technology, China Pharmaceutical University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282681105215746","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000020707674"}],"foaf:name":[{"@language":"en","@value":"YIN Dengke"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Pharmacy, Anhui University of Traditional Chinese Medicine"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282681105215750","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000020707681"}],"foaf:name":[{"@language":"en","@value":"ZHAN Fan"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Pharmacy, Anhui University of Traditional Chinese Medicine"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282681105215745","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000020707687"}],"foaf:name":[{"@language":"en","@value":"DAI Sufei"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Pharmacy, Anhui University of Traditional Chinese Medicine"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282681105215749","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000020707693"}],"foaf:name":[{"@language":"en","@value":"XIE Guangyan"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Pharmacy, Anhui University of Traditional Chinese Medicine"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282681105215744","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000020707703"}],"foaf:name":[{"@language":"en","@value":"SANG Xiaobing"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Pharmacy, Anhui University of Traditional Chinese Medicine"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00316903"},{"@type":"EISSN","@value":"13475231"},{"@type":"CODEN","@value":"YKKZAJ"},{"@type":"NDL_BIB_ID","@value":"000000023278"},{"@type":"ISSN","@value":"00316903"},{"@type":"LISSN","@value":"00316903"},{"@type":"NCID","@value":"AN00284903"}],"prism:publicationName":[{"@language":"en","@value":"YAKUGAKU ZASSHI"},{"@language":"en","@value":"YAKUGAKU ZASSHI"},{"@language":"ja","@value":"薬学雑誌"}],"dc:publisher":[{"@language":"en","@value":"The Pharmaceutical Society of Japan"},{"@language":"ja","@value":"公益社団法人 日本薬学会"}],"prism:publicationDate":"2010-09-01","prism:volume":"130","prism:number":"9","prism:startingPage":"1207","prism:endingPage":"1214"},"reviewed":"false","url":[{"@id":"http://id.ndl.go.jp/bib/10807207"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I10807207"},{"@id":"https://dl.ndl.go.jp/pid/10203935"},{"@id":"https://www.jstage.jst.go.jp/article/yakushi/130/9/130_9_1207/_pdf"},{"@id":"https://search.jamas.or.jp/link/ui/2010341127"}],"availableAt":"2010-09-01","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=human%20steroid%205%CE%B1-reductase%20type%20II%20purification","dc:title":"human steroid 5α-reductase type II purification"},{"@id":"https://cir.nii.ac.jp/all?q=screening%20model","dc:title":"screening model"},{"@id":"https://cir.nii.ac.jp/all?q=inhibitor","dc:title":"inhibitor"},{"@id":"https://cir.nii.ac.jp/all?q=mangiferin","dc:title":"mangiferin"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360011144002286848","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Biological Properties of Curcumin-Cellular and Molecular Mechanisms of Action"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011145213130368","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Stable expression of human 5α-reductase type II in COS1 cells due to chromosomal gene integration: a novel tool for inhibitor identification"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011146281591424","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"In vitro effects of mangiferin on superoxide concentrations and expression of the inducible nitric oxide synthase, tumour necrosis factor-α and transforming growth factor-β genes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292621218678784","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Tissue distribution and ontogeny of steroid 5 alpha-reductase isozyme expression."}]},{"@id":"https://cir.nii.ac.jp/crid/1360292621231605376","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Anti‐Androgen Effects of Extracts and Compounds from<i>Ganoderma lucidum</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360299770043082752","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"L’image du corps dans l’œuvre de Cronenberg"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574093609460736","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"5α-Reductase Isozymes in the Central Nervous System"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574093973363200","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"MOLECULAR MECHANISMS OF ACTION OF STEROID/THYROID RECEPTOR SUPERFAMILY MEMBERS"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574094872740608","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"In vitro inhibition of androstenedione 5α-reduction by finasteride in epithelium and stroma of human benign prostatic hyperplasia"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574094994146432","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Establishment of type II 5α-reductase over-expressing cell line as an inhibitor screening model"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574095489332864","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Activity of the Type 1 5α-Reductase Exhibits Regional Differences in Isolated Sebaceous Glands and Whole Skin"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855571317470208","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The curcuma antioxidants: pharmacological 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A review"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855571560802432","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Cloning, expression and characterization of rhesus macaque types 1 and 2 5alpha-Reductase: evidence for mechanism-Based inhibition by finasteride"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137043433031808","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Comparison of finasteride (proscar®), a 5α reductase inhibitor, and various commercial plant extracts in in vitro and in vivo 5α reductase inhibition"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981471209177728","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Mathematical model for the androgenic regulation of the prostate in intact and castrated adult male rats"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262943593286400","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A prospective study of the efficacy of Serenoa repens, Tamsulosin, and Serenoa repens plus Tamsulosin treatment for patients with benign prostate hyperplasia"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544420598895744","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Mechanism of 4-ene-steroid 5α-reductase proton transfer in androgen target tissues"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825893605207552","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Kinetic analysis of androstenedione 5α-reductase in epithelium and stroma of human prostate"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107370941632640","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Steroid 5α-reductase inhibitory activity of condensed tannins from woody plants"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388843496515584","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Inhibitory effect of traditional Chinese medicine Zi-Shen Pill on benign prostatic hyperplasia in rats"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388843716028416","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Genetic and pharmacological evidence for more than one human steroid 5 alpha-reductase."}]},{"@id":"https://cir.nii.ac.jp/crid/1363388845153767296","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Testosterone at High Concentrations Interacts with the Human Androgen Receptor Similarly to Dihydrotestosterone*"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670320595821696","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The hair growth promoting effect of Sophora flavescens extract and its molecular regulation"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204257231232","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Determination Method for Steroid 5.ALPHA.-Reductase Activity Using Liquid Chromatography/Atmospheric Pressure Chemical Ionization-Mass Spectrometry"},{"@value":"Determination method for steroid 5α-reductase activity using liquid chromatography/atmospheric pressure chemical ionization-mass spectrometry"},{"@language":"ja-Kana","@value":"Determination Method for Steroid 5 アルファ Reductase Activity Using Liquid Chromatography Atmospheric Pressure Chemical Ionization Mass Spectrometry"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204627175168","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Antidiabetic Activity of the Rhizoma of Anemarrhena asphodeloides and Active Components, Mangiferin and Its Glucoside."}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679603270784","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Testosterone 5.ALPHA.-Reductase Inhibitory Active Constituents from Anemarrhenae Rhizoma."},{"@value":"Tastosterone 5α-reductase inhibitory active constituents from Anemarrhenae Rhizoma"}]}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:0035541529"},{"@type":"NDL_SEARCH","@value":"oai:ndlsearch.ndl.go.jp:R000000004-I10807207"},{"@type":"CROSSREF","@value":"10.1248/yakushi.130.1207"},{"@type":"CIA","@value":"130000342381"}]}