ニワトリ型単クローン抗体を用いた肝細胞癌の診断

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  • 甲田 徹三
    国立病院呉医療センター臨床研究部 現:独立行政法人国立病院機構呉医療センター
  • 北條 慎太郎
    広島大学生物生産学部免疫生物学教室

書誌事項

タイトル別名
  • DIAGNOSIS OF HEPATOCELLULAR CARCINOMA USING CHICKEN ANTI-MONOCLONAL ANTIBODY
  • ニワトリガタタンクローン コウタイ オ モチイタ カン サイボウガン ノ シンダン

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説明

Siliac acid is a carbohydrate with 9 carbons, which is found on the terminal sugar chains of glycolipids and glycoproteins. It is known that sugar chains containing sialic acid play a role in intercellular signaling and in the recognition of cells and pathogens. N-acetylneuraminic acid and N-glycolylneuraminic acid (NeuGc) are the most widely detected sialic acids in mammals.<br>NeuGc is widely expressed in most mammals, but is not expressed in normal human or chicken tissues. NeuGc possesses immunogenicity in humans and chickens, and has attracted much attention as a tumor-associated antigen.<br>In this study, we performed the expression analysis of NeuGc in hepatocellualr carcinoma by immunohistochemistry method with chicken 2 monoclonal antibodies, Hu/Ch6-1 (against glycolipid type NeuGc), Hu/Ch2-7 (against glycolipid/glycoprotein type NeuGc).<br>In frozen liver sections from patients with hepatocellular carcinoma, glycolipid-type NeuGc was detected on the surface of liver cancer cells in 17 out of 37 samples (45.9%) by immunostaining using two chicken monoclonal antibodies against NeuGc and the tyramide signal amplification method. Increased serum levels of anti-NeuGc IgG and/or IgM were observed in 25 of the 37 patients with hepatocellular carcinoma (67.6%). The presence of these antibodies was mostly attributed to the expression of NeuGc on hepatocellular carcinoma cells. There was no correlation between serum AFP or PIVKAII levels and the presence of NeuGc or anti-NeuGc IgG and/or IgM.<br>We therefore consider that measurement of serum levels of anti-NeuGc antibodies is clinically meaningful and that anti-NeuGc antibody may be a useful screening test in combination with AFP and PIVKAII for diagnosis of hepatocellular carcinoma.

収録刊行物

  • 医療

    医療 58 (8), 470-476, 2004

    一般社団法人 国立医療学会

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