An Ultrastructural Study on the Effects of Mono(2-ethylhexyl) Phthalate on Mice Testes: Cell Death and Sloughing of Spermatogenic Cells

DOI PubMed 3 Citations 22 References Open Access
  • TAY Tat Wei
    Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • ANDRIANA Bibin Bintang
    Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • ISHII Maki
    Department of Developmental Neuroscience, Tokyo Metropolitan Institute for Neuroscience, Fuchu
  • CHOI Ehn Kyoung
    Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • ZHU Xiao Bo
    Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • ALAM Mohammad Shah
    Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • TSUNEKAWA Naoki
    Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • KANAI Yoshiakira
    Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • KUROHMARU Masamichi
    Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo

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Mono(2-ethylhexyl) phthalate (MEHP) is a well-characterized testicular toxicant. in this study, morphological alterations of mice testes caused by repeated administrations of MEHP were examined by light and transmission electron microscopy. Prepubertal male mice were given a range of MEHP doses (600-900 mg/kg/day) for 3 consecutive days in corn oil by oral gavage. Control animals were given only corn oil. Thereafter, the testes were excised, fixed in 4% paraformaldehyde for light microscopy and/or 5% glutaraldehyde for transmission electron microscopy. Then, they were embedded, and sectioned. TUNEL analysis was done to quantify the occurrence of apoptosis in the testis. Cellular damages were also observed. Results showed that administration of 700 mg/kg of MEHP caused a signifficant increase in TUNEL-positive cells. At the same time, mice treated with higher doses of MEHP showed presence of degenerating (apoptotic and necrotic) spermatogenic cells. Appearance of small vacuoles in the Sertoli cell cytoplasm and displacement of spermatogenic cells were also observed. Sloughed and shed spermatogenic cells found in the tubular lumen were identified to be necrotic and apoptotic in appearance, respectively.

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