An Ultrastructural Study on the Effects of Mono(2-ethylhexyl) Phthalate on Mice Testes: Cell Death and Sloughing of Spermatogenic Cells
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- TAY Tat Wei
- Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- ANDRIANA Bibin Bintang
- Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- ISHII Maki
- Department of Developmental Neuroscience, Tokyo Metropolitan Institute for Neuroscience, Fuchu
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- CHOI Ehn Kyoung
- Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- ZHU Xiao Bo
- Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- ALAM Mohammad Shah
- Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- TSUNEKAWA Naoki
- Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- KANAI Yoshiakira
- Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- KUROHMARU Masamichi
- Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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説明
Mono(2-ethylhexyl) phthalate (MEHP) is a well-characterized testicular toxicant. in this study, morphological alterations of mice testes caused by repeated administrations of MEHP were examined by light and transmission electron microscopy. Prepubertal male mice were given a range of MEHP doses (600-900 mg/kg/day) for 3 consecutive days in corn oil by oral gavage. Control animals were given only corn oil. Thereafter, the testes were excised, fixed in 4% paraformaldehyde for light microscopy and/or 5% glutaraldehyde for transmission electron microscopy. Then, they were embedded, and sectioned. TUNEL analysis was done to quantify the occurrence of apoptosis in the testis. Cellular damages were also observed. Results showed that administration of 700 mg/kg of MEHP caused a signifficant increase in TUNEL-positive cells. At the same time, mice treated with higher doses of MEHP showed presence of degenerating (apoptotic and necrotic) spermatogenic cells. Appearance of small vacuoles in the Sertoli cell cytoplasm and displacement of spermatogenic cells were also observed. Sloughed and shed spermatogenic cells found in the tubular lumen were identified to be necrotic and apoptotic in appearance, respectively.
収録刊行物
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- Ｏｋａｊｉｍａｓ Ｆｏｌｉａ Ａｎａｔｏｍｉｃａ Ｊａｐｏｎｉｃａ
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Ｏｋａｊｉｍａｓ Ｆｏｌｉａ Ａｎａｔｏｍｉｃａ Ｊａｐｏｎｉｃａ 83 (4), 123-130, 2007
オカジマ・フォリア・アナトミカ・ヤポニカ編集部