{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282681312531072.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1262/jrd.2013-015"}},{"identifier":{"@type":"COI","@value":"1:STN:280:DC%2BC3snjtFamsw%3D%3D"}},{"identifier":{"@type":"PMID","@value":"23666455"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"024797919"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/024797919"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I024797919"}},{"identifier":{"@type":"URI","@value":"https://www.jstage.jst.go.jp/article/jrd/59/4/59_2013-015/_pdf"}},{"identifier":{"@type":"NAID","@value":"10031195875"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/2014082908"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Comparison of Ethylene Glycol and Propylene Glycol for the Vitrification of Immature Porcine Oocytes"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"Our aim was to optimize a cryoprotectant treatment for vitrification of immature porcine cumulus-oocyte complexes (COCs). Immature COCs were vitrified either in 35% ethylene glycol (EG), 35% propylene glycol (PG) or a combination of 17.5% EG and 17.5% PG. After warming, the COCs were <i>in vitro</i> matured (IVM), and surviving oocytes were <i>in vitro</i> fertilized (IVF) and cultured. The mean survival rate of vitrified oocytes in 35% PG (73.9%) was higher (P<0.05) than that in 35% EG (27.8%). Oocyte maturation rates did not differ among vitrified and non-vitrified control groups. Blastocyst formation in the vitrified EG group (10.8%) was higher (P<0.05) than that in the vitrified PG group (2.0%) but was lower than that in the control group (25.0%). Treatment of oocytes with 35% of each cryoprotectant without vitrification revealed a higher toxicity of PG on subsequent blastocyst development compared with EG. The combination of EG and PG resulted in 42.6% survival after vitrification. The maturation and fertilization rates of the surviving oocytes were similar in the vitrified, control and toxicity control (TC; treated with EG+PG combination without cooling) groups. Blastocyst development in the vitrified group was lower (P<0.05) than that in the control and TC groups, which in turn had similar development rates (10.7%, 18.1% and 23.3%, respectively). In conclusion, 35% PG enabled a higher oocyte survival rate after vitrification compared with 35% EG. However, PG was greatly toxic to oocytes. The combination of 17.5% EG and 17.5% PG yielded reasonable survival rates without toxic effects on embryo development."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410282681312531080","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000263057813"}],"foaf:name":[{"@language":"en","@value":"SOMFAI Tamás"}],"jpcoar:affiliationName":[{"@language":"en","@value":"NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, 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Kazuhiro"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Division of Animal Sciences, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09168818"},{"@type":"LISSN","@value":"09168818"},{"@type":"EISSN","@value":"13484400"},{"@type":"NDL_BIB_ID","@value":"000000152532"},{"@type":"ISSN","@value":"09168818"},{"@type":"NCID","@value":"AA10936678"}],"prism:publicationName":[{"@language":"en","@value":"Journal of Reproduction and Development"},{"@language":"en","@value":"J. Reprod. Dev."},{"@language":"en","@value":"Journal of Reproduction and Development"}],"dc:publisher":[{"@language":"en","@value":"The Society for Reproduction and Development"},{"@language":"ja","@value":"公益社団法人 日本繁殖生物学会"}],"prism:publicationDate":"2013","prism:volume":"59","prism:number":"4","prism:startingPage":"378","prism:endingPage":"384"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://id.ndl.go.jp/bib/024797919"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I024797919"},{"@id":"https://www.jstage.jst.go.jp/article/jrd/59/4/59_2013-015/_pdf"},{"@id":"https://search.jamas.or.jp/link/ui/2014082908"}],"availableAt":"2013","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Cryoprotectant","dc:title":"Cryoprotectant"},{"@id":"https://cir.nii.ac.jp/all?q=Embryo%20development","dc:title":"Embryo development"},{"@id":"https://cir.nii.ac.jp/all?q=Immature%20oocyte","dc:title":"Immature oocyte"},{"@id":"https://cir.nii.ac.jp/all?q=%3Ci%3EIn%20vitro%3C/i%3E%20fertilization","dc:title":"<i>In vitro</i> fertilization"},{"@id":"https://cir.nii.ac.jp/all?q=Pig","dc:title":"Pig"},{"@id":"https://cir.nii.ac.jp/all?q=Vitrification","dc:title":"Vitrification"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050001202950318464","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Effects of polyethylene glycol and a synthetic ice blocker during vitrification of immature porcine oocytes on survival and subsequent embryo development."}]},{"@id":"https://cir.nii.ac.jp/crid/1360002219442171008","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Generation of Live Piglets from Cryopreserved Oocytes for the First Time Using a Defined System for In Vitro Embryo 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