{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282681318567296.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.5357/koubyou.75.29"}},{"identifier":{"@type":"PMID","@value":"18421949"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"9454231"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/9454231"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I9454231"}},{"identifier":{"@type":"NAID","@value":"130004283079"}},{"identifier":{"@type":"NAID","@value":"10024176434"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/2008182214"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Tissue Regeneration Induced by a Transgene for Bone Morphogenetic Protein-12 (BMP-12) in a Bone Defect"},{"@value":"骨欠損部へのＢＭＰ‐１２遺伝子導入による欠損部組織の再生"},{"@language":"ja-Kana","@value":"コツ ケッソンブ エノ BMP 12 イデンシ ドウニュウ ニ ヨル ケッソンブ ソシキ ノ サイセイ"}],"dc:language":"ja","description":[{"type":"abstract","notation":[{"@language":"en","@value":"BMP-12, other than BMP-2, 4, and 7, is known to induce the formation of tendon/ligament-like tissues. However, the role of the protein in the process of bone or cartilage healing has not been adequately examined. The purpose of the present study was to examine if a plasmid vector encoding BMP-12 would induce tendon/ ligament formation in a rat bone defect model. A lyophilized atelocollagen pellet with 100μg of a plasmid vector encoding BMP-12 or BMP-2 was implanted in a bone defect of a rat femoral metaphysis. The samples were retrieved at 2, 4 and 8 weeks post-operation and subjected to histology and real-time PCR analysis. BMP-12 transgene indicated prominent fibrogenesis at 2 weeks. Consequently, the tissue was substituted by bone at 8 weeks. The gene expression of collagen type III was up-regulated by BMP-12 gene transfer after 2 weeks, followed by an increase of elastin and Sixl genes after 4 weeks. These genes, which are intimately related to tendon/ligament formation, decreased in expression at 8 weeks in preparation for osteogenesis afterward. On the other hand, the BMP-2 transgene quickly induced the synthesis of bone-related genes such as collagen type I and ALP. It is concluded that BMP-12 can initiate the development of tendon/ligament-like tissue in a rat bone defect at early healing stages. Maintaining such generation of tissue on bone could be useful for modifying periodontal tissue engineering."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410572171321160064","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000014179517"},{"@type":"NRID","@value":"9000256428860"}],"foaf:name":[{"@language":"en","@value":"GOTO Nobuhiro"},{"@language":"ja","@value":"後藤 暢大"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Oral Implantology and Regenerative Dental Medicine, Department of Masticatory Function Rehabilitation, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University"},{"@language":"ja","@value":"東京医科歯科大学大学院医歯学総合研究科口腔機能再構築学系専攻摂食機能回復学講座インプラント・口腔再生医学分野"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"03009149"},{"@type":"EISSN","@value":"18845185"},{"@type":"NDL_BIB_ID","@value":"000000007679"},{"@type":"ISSN","@value":"03009149"},{"@type":"LISSN","@value":"03009149"},{"@type":"NCID","@value":"AN00302674"}],"prism:publicationName":[{"@language":"ja","@value":"口腔病学会雑誌"},{"@language":"en","@value":"THE JOURNAL OF THE STOMATOLOGICAL SOCIETY,JAPAN"},{"@language":"en","@value":"J. Stomatol. Soc. Jpn"},{"@language":"en","@value":"J. Stomatol.Soc.,Jpn."},{"@language":"ja","@value":"口病誌"}],"dc:publisher":[{"@language":"en","@value":"The Stomatological society, Japan"},{"@language":"ja","@value":"口腔病学会"}],"prism:publicationDate":"2008","prism:volume":"75","prism:number":"1","prism:startingPage":"29","prism:endingPage":"37"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://id.ndl.go.jp/bib/9454231"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I9454231"},{"@id":"https://search.jamas.or.jp/link/ui/2008182214"}],"availableAt":"2008","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=General%20Medicine","dc:title":"General Medicine"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360292619026513664","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"DNA delivery from polymer matrices for tissue engineering"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292620474763520","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Localized, direct plasmid gene delivery in vivo: prolonged therapy results in reproducible tissue regeneration"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981471021552384","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388846300994944","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Recombinant human bone morphogenetic protein induces bone formation."}]},{"@id":"https://cir.nii.ac.jp/crid/1363951796120459648","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Stimulation of new bone formation by direct transfer of osteogenic plasmid genes."}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680152351488","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"A New Technique With Calcium Phosphate Precipitate Enhances Efficiency of In Vivo Plasmid DNA Gene Transfer"},{"@language":"ja-Kana","@value":"New Technique With Calcium Phosphate Precipitate Enhances Efficiency of In Vivo Plasmid DNA Gene Transfer"}]},{"@id":"https://cir.nii.ac.jp/crid/1521136278477163392","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"Mesenchymal Stem Cells Differentiate into Tenocytes by Bone Morphogenetic Protein(BMP) 12 Gene Transfer"},{"@language":"ja-Kana","@value":"Mesenchymal Stem Cells Differentiate into Tenocytes by Bone Morphogenetic Protein BMP 12 Gene Transfer"}]},{"@id":"https://cir.nii.ac.jp/crid/1570009750790799360","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Tissue engineering via local gene delivery"}]},{"@id":"https://cir.nii.ac.jp/crid/1570291225767504896","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Bone : formation by autoinduction"}]},{"@id":"https://cir.nii.ac.jp/crid/1570572700091444352","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Atelocollagen-based gene transfer in cells allows high-throughput screening of gene functions"}]},{"@id":"https://cir.nii.ac.jp/crid/1570572700744219264","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"BMP-12 gene transfer augmentation of lacerated tendon repair"}]},{"@id":"https://cir.nii.ac.jp/crid/1570572700744220928","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Ectopic induction of tendon and ligament in rats by growth and differentiation factors 5, 6, and 7, members of the TGF-beta gene family"}]},{"@id":"https://cir.nii.ac.jp/crid/1570854175720926976","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"New delivery system for plasmid DNA in vivo using atelocollagen as a carrier material : the Minipellet"}]},{"@id":"https://cir.nii.ac.jp/crid/1570854175720931584","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Atelocollagen for protein and gene delivery"}]},{"@id":"https://cir.nii.ac.jp/crid/1570854176748844288","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The roles of bone morphogenetic protein (BMP) 12 in stimulating the proliferation and matrix production of human patellar tendon fibroblasts"}]},{"@id":"https://cir.nii.ac.jp/crid/1571135650804886016","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Biodegradability of 'Minipellet' a new drug formulation using atelocollagen as a drug carrier material, in the rhesus monkey"}]},{"@id":"https://cir.nii.ac.jp/crid/1571698600651063552","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Increased content of type III collagen at the rupture site of human Achilles tendon"}]},{"@id":"https://cir.nii.ac.jp/crid/1571980076617167744","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Recombinant human osteogenic protein-1 (hOP-1) induces new bone formation in vivo with a specific activity comparable with natural bovine osteogenetic protein and stimulates osteoblast proliferation and differentiation in vitro"}]},{"@id":"https://cir.nii.ac.jp/crid/1572543025581192320","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Tenocytes from ruptured and tendinopathic achilles tendons produce greater quantities of type III collagen than tenocytes from normal achilles tendons. An in vitro model of human tendon healing"}]},{"@id":"https://cir.nii.ac.jp/crid/1572543026353162240","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Bone regeneration by modified gene-activated matrix : effectiveness in segmental tibial defects in rats"}]},{"@id":"https://cir.nii.ac.jp/crid/1573105975534611712","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Localization of collagen types I, III and V during tendon development. Changes in collagen types I and III are correlated with changes in fibril diameter"}]},{"@id":"https://cir.nii.ac.jp/crid/1573105976530097152","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Bone morphogenetic protein-12 and 13 inhibit terminal differentiation of myoblasts, but do not induce their differentiation into osteoblasts"}]},{"@id":"https://cir.nii.ac.jp/crid/1573387450511321600","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"A comparative dose-response study of cartilage-derived morphogenetic protein (CDMP)-1, -2 and -3 for tendon healing in rats"}]},{"@id":"https://cir.nii.ac.jp/crid/1573387450511323904","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Collagen in tendon, ligament, and bone healing. A current review"}]},{"@id":"https://cir.nii.ac.jp/crid/1573950401462583168","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Production of functional human bone morphogenetic protein-2 using a baculovirus/Sf-9 insect cell system"}]},{"@id":"https://cir.nii.ac.jp/crid/1574231875441460096","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Effect of bone morphogenetic protein-12 gene transfer on mesenchymal progenitor cells"}]}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:0031239427"},{"@type":"NDL_SEARCH","@value":"oai:ndlsearch.ndl.go.jp:R000000004-I9454231"},{"@type":"CROSSREF","@value":"10.5357/koubyou.75.29"},{"@type":"PUBMED","@value":"18421949"},{"@type":"CIA","@value":"10024176434"},{"@type":"CIA","@value":"130004283079"},{"@type":"OPENAIRE","@value":"doi_dedup___::29868cf09d59783da5c84c86a487f6ba"}]}