{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282681385016192.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1679/aohc.61.151"}},{"identifier":{"@type":"COI","@value":"1:CAS:528:DyaK1cXjvF2rur8%3D"}},{"identifier":{"@type":"PMID","@value":"9650889"}},{"identifier":{"@type":"NAID","@value":"10026052527"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Immunohistochemical Identification of Type B Intercalated Cells in the Rat Kidney by a Monoclonal Antibody."}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"We recently produced monoclonal antibodies using macrophagic cells derived from cultured rat glomeruli as the antigen. One of the antibodies, named OS-3, was found to detect a cell population scattered in collecting ducts of the rat kidney as well as macrophages in various tissues. The present study deals with the cellular and subcellular localization of immunoreactivities with OS-3 in the kidney and other organs of rats. Double immunostaining using OS-3 and an antiserum against either calbindin or epidermal cytokeratin showed that OS-3-immunoreactive cells exist exclusively in both the connecting segment and cortical collecting duct, and differ from calbindin- or cytokeratinpositive epithelial cells. Ultrastructurally, OS-3-immunoreactive cells appeared spherical in shape with few cytoplasmic microprojections on the narrow apical surface. Their relatively dark cytoplasm contained numerous mitochondria and a developed tubulo-vesicular system. The intense immunoreactivity was selectively localized in the basolateral membrane exhibiting shallow but complicated infoldings. Distribution and ultrastructural properties of the OS-3-immunoreactive cells showed that they were type B intercalated cells, which are engaged in the regulation of the acid-base balance mainly by secreting HCO<sub>3</sub><sup>-</sup>. Another positive staining with OS-3 was found in the macula densa and some epithelial cells of Bowman's capsule, the former monitoring Cl<sup>-</sup> concentrations in the urine. Immunoblotting of extracts from the rat kidney demonstrated a protein band immunoreactive to OS-3 at a molecular weight of 43kDa. Aside from the kidney, a specific and intense immunoreactivity with OS-3 was also found in the epithelial cells of the pancreatic excretory duct and in the secretory cells of the salivary, pyloric and duodenal glands, all of which are HCO<sub>3</sub><sup>-</sup>-secreting cells. These immunohistochemical findings imply that OS-3 is useful for the detection of type B intercalated cells and recognizes a functional molecule involved in the production/secretion of HCO<sub>3</sub><sup>-</sup> or transport of Cl<sup>-</sup>."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410282681385016192","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000017172620"}],"foaf:name":[{"@language":"en","@value":"YANASE Haruko"},{"@language":"ja","@value":"簗瀬 晴子"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Laboratory of Anatomy, Graduate School of Veterinary Medicine, Hokkaido University"},{"@language":"ja","@value":"北海道大学大学院獣医学研究科解剖学教室"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658432363522","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"30185681"},{"@type":"NRID","@value":"1000030185681"},{"@type":"NRID","@value":"9000367853754"},{"@type":"NRID","@value":"9000283219502"},{"@type":"NRID","@value":"9000000532975"},{"@type":"NRID","@value":"9000391746231"},{"@type":"NRID","@value":"9000002366465"},{"@type":"NRID","@value":"9000265249768"},{"@type":"NRID","@value":"9000283705951"},{"@type":"NRID","@value":"9000009511417"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0188724"}],"foaf:name":[{"@language":"en","@value":"ORIKASA Michiaki"}],"jpcoar:affiliationName":[{"@language":"en","@value":"College of Biomedical Technology, Niigata University"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658312822658","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"40012728"},{"@type":"NRID","@value":"1000040012728"},{"@type":"NRID","@value":"9000283599415"},{"@type":"NRID","@value":"9000256092260"},{"@type":"NRID","@value":"9000006012498"},{"@type":"NRID","@value":"9000008475202"},{"@type":"NRID","@value":"9000021426719"},{"@type":"NRID","@value":"9000008424237"},{"@type":"NRID","@value":"9000000532669"},{"@type":"NRID","@value":"9000021880306"},{"@type":"NRID","@value":"9000009511802"},{"@type":"NRID","@value":"9000000613235"},{"@type":"NRID","@value":"9000006604840"},{"@type":"NRID","@value":"9000021319788"},{"@type":"NRID","@value":"9000004643407"},{"@type":"NRID","@value":"9000391591098"},{"@type":"NRID","@value":"9000326657128"},{"@type":"NRID","@value":"9000008350994"},{"@type":"NRID","@value":"9000255722092"},{"@type":"NRID","@value":"9000020547866"},{"@type":"NRID","@value":"9000017346591"},{"@type":"NRID","@value":"9000255720174"},{"@type":"NRID","@value":"9000000532944"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0009197"}],"foaf:name":[{"@language":"en","@value":"SHIMIZU Fujio"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Cell Biology, Institute of Nephrology, Niigata University School of Medicine"}]},{"@id":"https://cir.nii.ac.jp/crid/1420564276164748416","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"60242400"},{"@type":"NRID","@value":"1000060242400"},{"@type":"NRID","@value":"9000018697546"},{"@type":"NRID","@value":"9000248221547"},{"@type":"NRID","@value":"9000024322705"},{"@type":"NRID","@value":"9000241853053"},{"@type":"NRID","@value":"9000261669388"},{"@type":"NRID","@value":"9000107371061"},{"@type":"NRID","@value":"9000345200838"},{"@type":"NRID","@value":"9000405074533"},{"@type":"NRID","@value":"9000023088110"},{"@type":"NRID","@value":"9000001449243"},{"@type":"NRID","@value":"9000018238309"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0045854"}],"foaf:name":[{"@language":"en","@value":"KAWACHI Hiroshi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Cell Biology, Institute of Nephrology, Niigata University School of Medicine"}]},{"@id":"https://cir.nii.ac.jp/crid/1410282681385016195","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000017172621"}],"foaf:name":[{"@language":"en","@value":"IWANAGA Toshihiko"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Laboratory of Anatomy, Graduate School of Veterinary Medicine, Hokkaido University"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09149465"},{"@type":"EISSN","@value":"13491717"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/09149465"},{"@type":"NCID","@value":"AA1068990X"}],"prism:publicationName":[{"@language":"en","@value":"Archives of Histology and Cytology"},{"@language":"ja","@value":"Ａｒｃｈｉｖｅｓ　ｏｆ　Ｈｉｓｔｏｌｏｇｙ　ａｎｄ　Ｃｙｔｏｌｏｇｙ"},{"@language":"en","@value":"Arch. Histol. Cytol."},{"@language":"en","@value":"Archives of Histology and Cytology"},{"@language":"ja","@value":"Ａｒｃｈｉｖｅｓ　ｏｆ　Ｈｉｓｔｏｌｏｇｙ　ａｎｄ　Ｃｙｔｏｌｏｇｙ"}],"dc:publisher":[{"@language":"en","@value":"International Society of Histology and Cytology"},{"@language":"ja","@value":"国際組織細胞学会"}],"prism:publicationDate":"1998","prism:volume":"61","prism:number":"2","prism:startingPage":"151","prism:endingPage":"161"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","availableAt":"1998","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Histology","dc:title":"Histology"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1390282679264505600","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Immunohistochemical Demonstration of Cl- Pump in Type A Intercalated Cells of Rat Kidney."},{"@value":"Immunohistochemical Demonstration of Cl〔-〕 Pump in Type A Intercalated Cells of Rat Kidney"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681385786112","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Immunoreactivity to a Monoclonal Antibody (OS-3) Is Shared by Osteoclasts and Bicarbonate-Secreting Cells."}]},{"@id":"https://cir.nii.ac.jp/crid/1570009751121862400","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Mechanism of secretagogue-induced HCO_3^- and Cl^- loss from rat parotid acini"}]},{"@id":"https://cir.nii.ac.jp/crid/1570291226098573184","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Macrophagic cells outgrowth from normal rat glomerular culture : Possible metaplastic change from podocytes"}]},{"@id":"https://cir.nii.ac.jp/crid/1570291226707179008","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"An H^+-ATPase in opposite plasma membrane domains in subpopulations of kidney epithelial cell subpopulations"}]},{"@id":"https://cir.nii.ac.jp/crid/1570572701075279488","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Effect of acute respiratory acidosis on two populations of intercalated cells in rat cortical collecting duct"}]},{"@id":"https://cir.nii.ac.jp/crid/1570572701075285120","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Immunohistochemical localization of keratin in normal human tissues"}]},{"@id":"https://cir.nii.ac.jp/crid/1570854176051992704","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Structural-functional relationships along the distal nephron"}]},{"@id":"https://cir.nii.ac.jp/crid/1570854176051996032","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Macula densa cells sense luminal NaCl concentration via furosemide sensitive Na^+2Cl^-K^+ cotransport"}]},{"@id":"https://cir.nii.ac.jp/crid/1571135651028705536","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Mucus and bicarbonate secretion in the stomach and their possible role in mucosal protection"}]},{"@id":"https://cir.nii.ac.jp/crid/1571417126005411200","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Macula densa cell function"}]},{"@id":"https://cir.nii.ac.jp/crid/1571417126005412352","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Secretin causes H^+/HCO_3^- secretion from pig pancreatic ductules by vacuolar-type H^+-adenosine triphosphatase"}]},{"@id":"https://cir.nii.ac.jp/crid/1571698600982125056","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Intracellular pH in the rat mandibular salivary gland : the role of Na-H and Cl-HCO_3 antiports in secretion"}]},{"@id":"https://cir.nii.ac.jp/crid/1571698600982128256","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Activities and subcellular localizations of enzymes implicated in gastroduodenal bicarbonate secretion"}]},{"@id":"https://cir.nii.ac.jp/crid/1571980075958831744","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Axial distribution of band 3-positive intercalated cells in the collecting duct of control and ammonium chloride-loaded rabbits"}]},{"@id":"https://cir.nii.ac.jp/crid/1571980075958835456","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Two types of collecting duct mitochondria-rich (intercalated) cells : lectin and band 3 cytochemistry"}]},{"@id":"https://cir.nii.ac.jp/crid/1572261550935543424","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1572261550935547520","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Immunocytochemical localization of band 3 protein in the rat collecting duct"}]},{"@id":"https://cir.nii.ac.jp/crid/1572543026532076416","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Subtypes of intercalated cells in rat kidney collecting duct delined by antibodies against erythroid band 3 and renal vacuolar H^+ -ATPase"}]},{"@id":"https://cir.nii.ac.jp/crid/1572824500888966528","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Immunocytochemical localization of vitamin D-dependent calcium-binding protein in renal tubules of rabbit, rat, and chick"}]},{"@id":"https://cir.nii.ac.jp/crid/1572824500888969728","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Function and regulation of collecting duct intercalated cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1573105975865678848","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Keratin cytoskeltons in epithelial cells of internal organs"}]},{"@id":"https://cir.nii.ac.jp/crid/1573105975865680000","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Massive proteinuria induced in rats by a single intravenous injection of a monoclonal antibody"}]},{"@id":"https://cir.nii.ac.jp/crid/1573387450842386432","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Prostaglandins and alkaline secretion from oxyntic, antral, and duodenal mucosa of the dog"}]},{"@id":"https://cir.nii.ac.jp/crid/1573387450842388736","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Cortical collecting duct bicarbonate secretion"}]},{"@id":"https://cir.nii.ac.jp/crid/1573950400795811200","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Morphological adaptation of the collecting duct to acid-base disturbances"}]},{"@id":"https://cir.nii.ac.jp/crid/1573950400795812224","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Effect of vasoactive intestinal polypeptide and somatostatin on secretion of epidermal growth factor and bicarbonate from Brunner's glands"}]},{"@id":"https://cir.nii.ac.jp/crid/1573950400795813376","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The origin of and subcellular mechanisms causing pancreatic bicarbonate secretion"}]},{"@id":"https://cir.nii.ac.jp/crid/1574231875772520832","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4"}]},{"@id":"https://cir.nii.ac.jp/crid/1574231875772522240","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Organization of collecting duct intercalated cells"}]}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:0008495639"},{"@type":"CROSSREF","@value":"10.1679/aohc.61.151"},{"@type":"PUBMED","@value":"9650889"},{"@type":"CIA","@value":"10026052527"},{"@type":"OPENAIRE","@value":"doi_dedup___::3ec9a2396fafdca0c2da5c1b31192d86"},{"@type":"CROSSREF","@value":"10.1679/aohc.63.255_references_DOI_LMh3qBiNX8XzJLetye9eDwOSJlT"},{"@type":"CROSSREF","@value":"10.1254/jjp.83.233_references_DOI_LMh3qBiNX8XzJLetye9eDwOSJlT"}]}