{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282681387533952.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.2331/suisan.48.57"}},{"identifier":{"@type":"URI","@value":"https://agriknowledge.affrc.go.jp/RN/2010242594"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"2437293"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/2437293"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I2437293"}},{"identifier":{"@type":"NAID","@value":"130001547143"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"魚類ミオシンおよびミオシンＢのＡＴＰａｓｅ活性に及ぼすＮ‐エチルマレイミド修飾の影響"},{"@language":"en","@value":"Effects of N-ethylmaleimide Modification on ATPase Activities of Fish Myosin and Myosin B"},{"@language":"ja-Kana","@value":"ギョルイ ミオシン オヨビ ミオシン B ノ ATPase カッセイ ニ オヨ"}],"dc:language":"ja","description":[{"type":"abstract","notation":[{"@language":"en","@value":"The Ca<sup>2+</sup> -ATPase activity of fish myosin was activated by N-ethylmaleimide (NEM) treatment, while the EDTA-ATPase activity was inhibited. These changes indicate that fish myosin, like rabbit myosin, contains first reactive thiol groups (SH<sub>1</sub>).<br> On the other hand, myosin SH<sub>1</sub> in myosin B system was completely unreactive with NEM under the conditions where the association of actin-myosin occurred, i.e., at physiological ionic strength and pH, and in the absence of ATP. These results suggest that myosin SH<sub>1</sub> in fish muscle may be masked with actin under the conditions of postmortem storage. The Mg<sup>2+</sup> -ATPase activtity of myosin B, however, increased by NEM treatment under the conditions where SH<sub>1</sub> was unreactive. <br> It was also found that the thermal stability of the ATPase activity of fish myosin B. on storage at high ionic strength, was decreased remarkably by the NEM treatment. This thermal instability of the NEM treated myosin B may be due to the dissociation of myosin and actin."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410282681387533952","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000020016785"}],"foaf:name":[{"@language":"en","@value":"WATANABE Takahiro"},{"@language":"ja","@value":"渡辺 孝博"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Faculty of Fisheries, Hokkaido 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