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Polymerase Chain Reaction-Based Restriction Fragment Length Polymorphism Pattern of Porcine Reproductive and Respiratory Syndrome Virus Directly from Lung Tissues without Virus Isolation in Korea.
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- CHEON Doo-Sung
- Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
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- CHAE Chanhee
- Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
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Description
Polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) analysis was developed for directly typing porcine reproductive and respiratory syndrome virus (PRRSV) from lung specimens without virus isolation. Twenty nine lung specimens collected from postweaning pigs were isolated for PRRSV. When the PCR products from the 29 lung specimens were digested by the restriction enzymes MluI, HincII, SacII and HaeIII, the RFLP patterns from the 29 lung specimens matched with those from the corresponding PRRSV isolates from each pig. The results suggest that the PCR-based RFLP analysis method may be useful to distinguish PRRSV isolates directly from lung specimens without virus isolation.
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 63 (5), 567-571, 2001
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Keywords
Details 詳細情報について
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- CRID
- 1390282681402587008
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- NII Article ID
- 110003920653
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- NII Book ID
- AN00191788
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- COI
- 1:CAS:528:DC%2BD3MXkslGqt7k%3D
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 5773957
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- PubMed
- 11411506
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- JaLC
- NDL Search
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed