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Strain OxB-1."},{"@value":"High Yield Synthesis of Nitriles by a New Enzyme, Phenylacetaldoxime Dehydratase, from Bacillus sp. Strain OxB-1"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"3-Phenylpropionitrile was synthesized from Z-3-phenylpropionaldoxime (0.75 M) in a quantitative yield (98 g/l) by the use of cells of Eschrichia coli JM 109/pOxD-9OF, a transformant harboring a gene for a new enzyme, phenylacetaldoxime dehydratase, from Bacillus sp. strain OxB-1. Other arylalkyl- and alkyl-nitriles were also synthesized in high yields from the corresponding aldoximes. Moreover, 3-phenylpropionitrile was successfully synthesized by the recombinant cells in 70 and 100% yields from 0.1 M unpurified E/Z-3-phenylpropionaldoxime, which is spontaneously formed from 3-phenylpropionaldehyde and hydroxylamine in a butyl acetate/water biphasic system and aqueous phase, respectively."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420845751137063168","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"20254237"},{"@type":"NRID","@value":"1000020254237"},{"@type":"NRID","@value":"9000002146990"},{"@type":"NRID","@value":"9000291274289"},{"@type":"NRID","@value":"9000001371365"},{"@type":"NRID","@value":"9000018661010"},{"@type":"NRID","@value":"9000021905773"},{"@type":"NRID","@value":"9000018563642"},{"@type":"NRID","@value":"9000238411196"},{"@type":"NRID","@value":"9000256199468"},{"@type":"NRID","@value":"9000253175349"},{"@type":"NRID","@value":"9000014273215"},{"@type":"NRID","@value":"9000004123646"},{"@type":"NRID","@value":"9000409807823"},{"@type":"NRID","@value":"9000254931605"},{"@type":"NRID","@value":"9000016402451"},{"@type":"NRID","@value":"9000261040154"},{"@type":"NRID","@value":"9000021106838"},{"@type":"NRID","@value":"9000404814424"},{"@type":"NRID","@value":"9000007039674"},{"@type":"NRID","@value":"9000018768445"},{"@type":"NRID","@value":"9000242073267"},{"@type":"NRID","@value":"9000283629566"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0045046"}],"foaf:name":[{"@language":"en","@value":"KATO 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Yasuhisa"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Biotechnology Research Center, Toyama Prefectural University"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09168451"},{"@type":"EISSN","@value":"13476947"},{"@type":"NDL_BIB_ID","@value":"000000151899"},{"@type":"ISSN","@value":"09168451"},{"@type":"LISSN","@value":"09168451"},{"@type":"NCID","@value":"AA10824164"}],"prism:publicationName":[{"@language":"en","@value":"Bioscience, Biotechnology, and Biochemistry"},{"@language":"ja","@value":"Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ"},{"@language":"en","@value":"Biosci. Biotechnol. Biochem."},{"@language":"en","@value":"Bioscience, Biotechnology, and Biochemistry"},{"@language":"ja","@value":"Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ"}],"dc:publisher":[{"@language":"en","@value":"Japan Society for Bioscience, Biotechnology, and Agrochemistry"},{"@language":"ja","@value":"公益社団法人 日本農芸化学会"}],"prism:publicationDate":"2001","prism:volume":"65","prism:number":"12","prism:startingPage":"2666","prism:endingPage":"2672"},"reviewed":"false","url":[{"@id":"http://id.ndl.go.jp/bib/6027298"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I6027298"},{"@id":"http://www.tandfonline.com/doi/pdf/10.1271/bbb.65.2666"}],"availableAt":"2001","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=phenylacetaldoxime%20dehydratase","dc:title":"phenylacetaldoxime dehydratase"},{"@id":"https://cir.nii.ac.jp/all?q=3-phenylpropionitrile","dc:title":"3-phenylpropionitrile"},{"@id":"https://cir.nii.ac.jp/all?q=aldehyde","dc:title":"aldehyde"},{"@id":"https://cir.nii.ac.jp/all?q=3-phenylpropionaldoxime","dc:title":"3-phenylpropionaldoxime"},{"@id":"https://cir.nii.ac.jp/all?q=Bacillus%20sp.%20strain%20OxB-1","dc:title":"Bacillus sp. strain OxB-1"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002214460643456","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Biocatalytic Synthesis of Nitriles through Dehydration of Aldoximes: The Substrate Scope of Aldoxime Dehydratases"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002217131927296","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Crystal structure of aldoxime dehydratase and its catalytic mechanism involved in carbon-nitrogen triple-bond synthesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360013168741511296","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Cyanide-Free Enantioselective Catalytic Strategies for the Synthesis of Chiral Nitriles"}]},{"@id":"https://cir.nii.ac.jp/crid/1360013168741527424","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Approaching Bulk Chemical Nitriles from Alkenes: A Hydrogen Cyanide-Free Approach through a Combination of Hydroformylation and Biocatalysis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360014093957416448","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Rationalizing the Unprecedented Stereochemistry of an Enzymatic Nitrile Synthesis through a Combined Computational and Experimental Approach"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285710949440640","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Complete Genome Sequence of an Aldoxime Degrader, Bacillus sp. 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