Isolation and Identification of Antimicrobial Compounds in Brazilian Propolis.

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Three distinct antimicrobial compounds were isolated from Brazilian propolis. These compounds were identified as 3, 5-diprenyl-4-hydroxycinnamic acid (1), 3-prenyl-4-dihydrocinnamoloxycinnamic acid (2), and 2, 2-dimethyl-6-carboxyethenyl-2H-1-benzopyran (3). The respective antimicrobial activity, expressed as MIC in μg/ml, 1-3 against Bacillus cereus was 15. 6, 31.3, and 125 ; that against Enterobacter aerogenes was 31.3, 62.5, and 125 ; and that against Arthroderma benhamiae was 15.6, >250, and 62.5. Compound 1 is likely to be one of the major antimicrobial compounds in Brazilian propolis.

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