Mouse Epidermal Keratinocytes in Three-Dimensional Organotypic Coculture with Dermal Fibroblasts Form a Stratified Sheet Resembling Skin

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  • IKUTA Shuzo
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • SEKINO Nobufumi
    Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • HARA Takeshi
    Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • CHIDA Kazuhiro
    Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • SAITO Yuriko
    Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo

Bibliographic Information

Published
2006
Resource Type
journal article
DOI
  • 10.1271/bbb.60266
Publisher
Japan Society for Bioscience, Biotechnology, and Agrochemistry

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Description

We describe an organotypic model of mouse skin consisting of a stratified sheet of epidermal keratinocytes and dermal fibroblasts within a contracted collagen gel. The model was designed to maintain the polarity of stratified keratinocytes and permit their long-term culture at an air-liquid interface. After air exposure, the thickness of the keratinocyte sheet transiently increased and then decreased to two cell layers at 2 weeks. The two-cell-layer structure is similar to that of the adult mouse epidermis. Cytokeratin 5 was localized in the lowest cell layer in the epithelial sheet, but cytokeratin 1 and loricrin were localized in the outer cell layers, resembling mouse skin. The expressions of interleukin 1α and 1β in the keratinocytes and of keratinocyte growth factor 1 and 2 in the fibroblasts correlated with keratinocyte stratification. The mouse organotypic coculture is useful in studying epithelial cell-mesenchymal cell interactions in vitro.

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