{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390282681454946560.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1271/bbb.110240"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"023328323"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/023328323"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I023328323"}},{"identifier":{"@type":"URI","@value":"http://www.tandfonline.com/doi/pdf/10.1271/bbb.110240"}},{"identifier":{"@type":"NAID","@value":"10030396348"}}],"dc:title":[{"@language":"en","@value":"Purification and Characterization of a Novel (S)-Enantioselective Transaminase from Pseudomonas fluorescens KNK08-18 for the Synthesis of Optically Active Amines"},{"@value":"Purification and Characterization of a Novel (<i>S</i>)-Enantioselective Transaminase from<i>Pseudomonas fluorescens</i>KNK08-18 for the Synthesis of Optically Active Amines"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"<I>Pseudomonas fluorescens</I> KNK08-18, showing (<I>S</I>)-selective transaminase activity, was isolated from soil by an enrichment culture method using (<I>S</I>)-7-methoxy-2-aminotetraline as the main nitrogen source. A transaminase was purified from the strain to homogeneity in seven steps. The relative mass of the enzyme was estimated to be 53 kDa on SDS-polyacrylamide gel electrophoresis and 120 kDa by gel filtration, suggesting a homodimeric structure. The optimal pH and temperature for enzyme activity were about 8.0–8.5 and 40 °C. <BR>The purified enzyme produced (<I>S</I>)-7-methoxy-2-aminotetraline, (<I>S</I>)-SMA, from 7-methoxy-2-tetralone (SMT) with high enantioselectivity. Although (<I>S</I>)-1-phenylethylamine was the best amino donor, β-alanine and 4-aminobutyric acid, which are good substrates for typical ω-amino acid transaminase (EC 2.6.1.18) and GABA transaminase (2.6.1.19), were not reacted. It aminated a broad range of carbonyl compounds containing aromatic, non-aromatic, and acidic and non-acidic substrates."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410282681454946561","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000019089652"}],"foaf:name":[{"@language":"en","@value":"ITO Noriyuki"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Frontier Biochemical and Medical Research Laboratories, Kaneka Corporation"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001206477872000","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000004204200"},{"@type":"NRID","@value":"9000255961646"}],"foaf:name":[{"@language":"en","@value":"KAWANO Shigeru"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Frontier Biochemical and Medical Research Laboratories, Kaneka Corporation"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001206474226944","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000004143475"},{"@type":"NRID","@value":"9000255961647"}],"foaf:name":[{"@language":"en","@value":"HASEGAWA Junzo"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Frontier Biochemical and Medical Research Laboratories, Kaneka Corporation"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001205311198976","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000004177228"},{"@type":"NRID","@value":"9000255961648"}],"foaf:name":[{"@language":"en","@value":"YASOHARA Yoshihiko"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Frontier Biochemical and Medical Research Laboratories, Kaneka Corporation"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09168451"},{"@type":"EISSN","@value":"13476947"},{"@type":"NDL_BIB_ID","@value":"000000151899"},{"@type":"ISSN","@value":"09168451"},{"@type":"LISSN","@value":"09168451"},{"@type":"NCID","@value":"AA10824164"}],"prism:publicationName":[{"@language":"en","@value":"Bioscience, Biotechnology, and Biochemistry"},{"@language":"ja","@value":"Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ"},{"@language":"en","@value":"Biosci. Biotechnol. Biochem."},{"@language":"en","@value":"Bioscience, Biotechnology, and Biochemistry"},{"@language":"ja","@value":"Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ"}],"dc:publisher":[{"@language":"en","@value":"Japan Society for Bioscience, Biotechnology, and Agrochemistry"},{"@language":"ja","@value":"公益社団法人 日本農芸化学会"}],"prism:publicationDate":"2011","prism:volume":"75","prism:number":"11","prism:startingPage":"2093","prism:endingPage":"2098"},"reviewed":"false","url":[{"@id":"http://id.ndl.go.jp/bib/023328323"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I023328323"},{"@id":"http://www.tandfonline.com/doi/pdf/10.1271/bbb.110240"}],"availableAt":"2011","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=purification","dc:title":"purification"},{"@id":"https://cir.nii.ac.jp/all?q=%CF%89-transaminase","dc:title":"ω-transaminase"},{"@id":"https://cir.nii.ac.jp/all?q=%3CI%3EPseudomonas%20fluorescens%3C/I%3E","dc:title":"<I>Pseudomonas fluorescens</I>"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1361137044926028800","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@value":"ω-Amino Acid:Pyruvate Transaminase from\n            <i>Alcaligenes denitrificans</i>\n            Y2k-2: a New Catalyst for Kinetic Resolution of β-Amino Acids and Amines"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825894289999232","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Industrial Biotransformations"}]},{"@id":"https://cir.nii.ac.jp/crid/1570009751828708736","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1570291225804916352","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1570291226813894144","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1571417125711754880","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1571417125711757952","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1571417125711758976","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1571417126437192320","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1571417126437199360","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1571698600688465280","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1571698600688466560","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1571698600688468864","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1571698600688470016","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1571698600688471040","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1571698601677709696","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1572261550641892608","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1572261551382558080","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1572261551580261888","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1572543025618599168","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1572543025618603392","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1572824500595311104","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1573387450548728832","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1573668925525441024","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1573668925525443456","@type":"Article","relationType":["cites"]}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:0038277017"},{"@type":"NDL_SEARCH","@value":"oai:ndlsearch.ndl.go.jp:R000000004-I023328323"},{"@type":"CROSSREF","@value":"10.1271/bbb.110240"},{"@type":"CIA","@value":"10030396348"}]}