Expression in Escherichia coli, Refolding, and Purification of the Recombinant Mature Form of Human Matrix Metalloproteinase 7 (MMP-7)
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- MUTA Yuko
- Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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- YASUI Natsuki
- Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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- MATSUMIYA Yoshiki
- Department of Biotechnology, College of Life Sciences, Ritsumeikan University
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- KUBO Motoki
- Department of Biotechnology, College of Life Sciences, Ritsumeikan University
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- INOUYE Kuniyo
- Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
Bibliographic Information
- Other Title
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- Expression in<i>Escherichia coli</i>, Refolding, and Purification of the Recombinant Mature Form of Human Matrix Metalloproteinase 7 (MMP-7)
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Description
In the latent pro-form of matrix metalloproteinase 7 (MMP-7), the cysteine residue in the pro-peptide binds the active-site zinc ion. Hence, recombinant active MMP-7 was prepared from pro-MMP-7 by modification of this cysteine residue with a mercuric reagent. In this study, mature MMP-7 was expressed in Escherichia coli as inclusion bodies, solubilized, and refolded with 1 M L-arginine. The purified product was indistinguishable from the one prepared from pro-MMP-7 as assessed by hydrolysis of (7-methoxycoumarin-4-yl)acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 74 (12), 2515-2517, 2010
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282681455801984
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- NII Article ID
- 10027728471
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- HANDLE
- 2433/156366
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- NDL BIB ID
- 10928685
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed