dsRNA-seq Reveals Novel RNA Virus and Virus-Like Putative Complete Genome Sequences from <i>Hymeniacidon sp.</i> Sponge

  • Urayama Syun-ichi
    Research Center for Bioscience and Nanoscience (CeBN), Japan Agency for Marine-Earth Science and Technology (JAMSTEC) Laboratory of Fungal Interaction and Molecular Biology (donated by IFO), Department of Life and Environmental Sciences, University of Tsukuba Microbiology Research Center for Sustainability (MiCS), University of Tsukuba
  • Takaki Yoshihiro
    Super-cutting-edge Grand and Advanced Research (SUGAR) Program
  • Hagiwara Daisuke
    Laboratory of Fungal Interaction and Molecular Biology (donated by IFO), Department of Life and Environmental Sciences, University of Tsukuba Microbiology Research Center for Sustainability (MiCS), University of Tsukuba
  • Nunoura Takuro
    Research Center for Bioscience and Nanoscience (CeBN), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

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Other Title
  • dsRNA-seq Reveals Novel RNA Virus and Virus-Like Putative Complete Genome Sequences from Hymeniacidon sp. Sponge

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Abstract

<p>Invertebrates are a source of previously unknown RNA viruses that fill gaps in the viral phylogenetic tree. Although limited information is currently available on RNA viral diversity in the marine sponge, a primordial multicellular animal that belongs to the phylum Porifera, the marine sponge is one of the well-studied holobiont systems. In the present study, we elucidated the putative complete genome sequences of five novel RNA viruses from Hymeniacidon sponge using a combination of double-stranded RNA sequencing, called fragmented and primer ligated dsRNA sequencing, and a conventional transcriptome method targeting single-stranded RNA. We identified highly diverged RNA-dependent RNA polymerase sequences, including a potential novel RNA viral lineage, in the sponge and three viruses presumed to infect sponge cells.</p>

Journal

  • Microbes and Environments

    Microbes and Environments 35 (2), n/a-, 2020

    Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

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