{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390285300151790208.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.2485/jhtb.29.111"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"030422763"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/030422763"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I030422763"}},{"identifier":{"@type":"URI","@value":"https://www.jstage.jst.go.jp/article/jhtb/29/2/29_111/_pdf"}},{"identifier":{"@type":"NAID","@value":"130007833314"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/2021069113"}}],"dc:title":[{"@language":"en","@value":"Effects of Adipose Tissue-Derived Stem Cells Transplanted to a Bone Defect after Irradiation"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"<p>We examined whether the effect of adipose tissue-derived stem cell (ADSC) transplantation improved bone wounds healing irradiation. ADSCs were harvested from F344 rats and the cells cultured until the second passage for transplantation. Before ADSC transplantation, a single dose of 15 Gy irradiation was administered to the head of every rat using <sup>137</sup>Cs gamma-ray irradiation system, except for the rats of the control group. Two weeks after the irradiation, ADSCs were seeded on a carrier (collagen sponge) and transplanted into the bone defects formed on the rat parietal bones (the irradiation transplant group). This group was compared with two other groups: the carrier only group (without ADSCs) implanted after irradiation (the irradiation group) and; the group in which the carrier was implanted without irradiation (the control group). The results were obtained by histological, immunohistochemistry and ultrastructural observation. We examined the effects of ADSC transplantation on the delay of bone wound healing after irradiation. The new bone formation area of the irradiation group was significantly suppressed (p <0.05) as compared with the other groups. The vascular density at the site of new bone formation in the irradiation group decreased as compared with the other groups. In the irradiation transplant group, the BrdU positive cells were arranged like osteoblasts at the newly formed bone area and were also seen in the vascular wall and the interstitial tissue at the bone defect site. In the irradiation transplant group, VEGF positive cells appeared in blood vessels and the interstitial tissue in the bone defect area, whereas in the other groups, they were hardly found. ADSCs improved bone wound healing after irradiation by direct differentiation of bone forming cells and vascular endothelial cells. In addition, ADSCs have a paracrine effect which induces cell differentiation into VEGF positive cells which acts on angiogenesis.</p>"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410285300151790211","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000408793373"}],"foaf:name":[{"@language":"en","@value":"Inazumi Mikako"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Oral and Maxillofacial Surgery, Asahikawa Medical University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410285300151790209","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000408793374"}],"foaf:name":[{"@language":"en","@value":"Takekawa Masanori"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Oral and Maxillofacial Surgery, Asahikawa Medical University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410285300151790208","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000408793375"}],"foaf:name":[{"@language":"en","@value":"Oka Kumiko"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Oral and Maxillofacial Surgery, Asahikawa Medical University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410285300151790210","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000408793376"}],"foaf:name":[{"@language":"en","@value":"Shibayama Naohiro"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Oral and Maxillofacial Surgery, Asahikawa Medical University"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13417649"},{"@type":"EISSN","@value":"1880828X"},{"@type":"CODEN","@value":"JHTBFF"},{"@type":"NDL_BIB_ID","@value":"000000099929"},{"@type":"ISSN","@value":"13417649"},{"@type":"LISSN","@value":"13417649"},{"@type":"NCID","@value":"AA11074332"}],"prism:publicationName":[{"@language":"en","@value":"Journal of Hard Tissue Biology"},{"@language":"en","@value":"J. hard tissue biol."},{"@language":"en","@value":"J. Hard Tissue Biology."}],"dc:publisher":[{"@language":"en","@value":"THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY"},{"@language":"ja","@value":"硬組織再生生物学会"}],"prism:publicationDate":"2020","prism:volume":"29","prism:number":"2","prism:startingPage":"111","prism:endingPage":"122"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://id.ndl.go.jp/bib/030422763"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I030422763"},{"@id":"https://www.jstage.jst.go.jp/article/jhtb/29/2/29_111/_pdf"},{"@id":"https://search.jamas.or.jp/link/ui/2021069113"}],"availableAt":"2020","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Adipose%20tissue-derived%20stem%20cells","dc:title":"Adipose tissue-derived stem cells"},{"@id":"https://cir.nii.ac.jp/all?q=Bone%20regeneration","dc:title":"Bone regeneration"},{"@id":"https://cir.nii.ac.jp/all?q=Radiation%20injury","dc:title":"Radiation injury"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050290384124569984","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Comparing the Osteogenic Potential and Bone Regeneration Capacities of Dedifferentiated Fat Cells and Adipose-Derived Stem Cells In Vitro and In Vivo: Application of DFAT Cells Isolated by a Mesh Method"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011143837827456","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Paracrine Regulation of Angiogenesis and Adipocyte Differentiation During In Vivo Adipogenesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292619483708416","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Viability and angiogenic activity of mesenchymal stromal cells from adipose tissue and bone marrow under hypoxia and inflammation in vitro"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574095990208896","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Role of adipose‐derived stem cells in wound healing"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855570075731584","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Adipose-Derived Stem Cells and Their Secretory Factors as a Promising Therapy for Skin Aging"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044778543744","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The MSC: An Injury Drugstore"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044981286656","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Novel Autologous Cell Therapy in Ischemic Limb Disease Through Growth Factor Secretion by Cultured Adipose Tissue–Derived Stromal Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699994483860864","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Stem Cell Therapy Remediates Reconstruction of the Craniofacial Skeleton After Radiation Therapy"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981470574323456","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Locally Administered Adipose-Derived Stem Cells Accelerate Wound Healing through Differentiation and Vasculogenesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262943792895616","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Why are MSCs therapeutic? New data: new insight"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262944252767232","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Adipose-Derived Stem Cells Protect Skin Flaps against Ischemia/Reperfusion Injury via IL-6 Expression"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419587364352","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Scanning electron microscopy of primary membrane bone"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544420231672576","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"ADSC-conditioned media elicit an ex vivo anti-inflammatory macrophage response"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825893671436544","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"THE ROLE OF THE VESSELS IN OSTEOGENESIS"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825895841524224","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Hypoxic culture and in vivo inflammatory environments affect the assumption of pericyte characteristics by human adipose and bone marrow progenitor cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825896020231680","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Bone formation using human adipose tissue‐derived stromal cells and a biodegradable scaffold"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388843212488448","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Bone marrow mesenchymal stem cell transplantation improves radiation-induced heart injury through DNA damage repair in rat model"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388844927373824","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Human adipose tissue-derived stem cells differentiate into endothelial cells in vitro and improve postnatal neovascularization in vivo"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670318808407296","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Comparison of Human Mesenchymal Stem Cells Derived From Adipose Tissue and Bone Marrow for Ex Vivo Gene Therapy in Rat Spinal Fusion Model"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670319634922624","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Cell Therapy Based on Adipose Tissue-Derived Stromal Cells Promotes Physiological and Pathological Wound Healing"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951793718320000","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Radiosensitivity of capillary endothelium"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233271189327104","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Transplantation of BMSCs expressing hPDGF-A/hBD2 promotes wound healing in rats with combined radiation-wound injury"}]}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:2007762278"},{"@type":"NDL_SEARCH","@value":"oai:ndlsearch.ndl.go.jp:R000000004-I030422763"},{"@type":"CROSSREF","@value":"10.2485/jhtb.29.111"},{"@type":"CIA","@value":"130007833314"},{"@type":"OPENAIRE","@value":"doi_dedup___::020b906a35608ac07c2de2b7cb364974"},{"@type":"CROSSREF","@value":"10.3390/ijms222212392_references_DOI_988AUwapIvEdQnrHVW7SJCczs0s"}]}