Cannabidiol (CBD) in cannabis has an anti-inflammatory effect, and its receptor, G protein-coupled receptor 55 (GPR55), has been suggested to be involved in the regulation of inflammation. Here, we investigated GPR55 expression in human gingival fibroblasts (HGFs) and anti-inflammatory effects of CBD via GPR55.
GPR55 expression and localization were confirmed in control, LPS-stimulated (LPS), CBD-treated and LPS-stimulated (CBD+LPS), and CBD-treated (CBD) groups. We also compared interleukin (IL) -6 and IL-8 protein production in each group.
GPR55 expression levels were significantly reduced in the LPS and CBD+LPS groups (p<0.05) compared to control and CBD groups, and GPR55 protein was found diffusely in the cytoplasm of HGFs in all groups. In addition, IL-6 and IL-8 production was significantly reduced in non-knockdown HGFs in the CBD+LPS group compared to the LPS group (p<0.05). By contrast, in GPR55-knockdown HGFs, there was a significant increase in IL-6 and IL-8 production in the LPS and CBD+LPS groups compared with the control groups (p<0.05), but there was no significant difference in IL-6 and IL-8 production in GPR55-knockdown HGFs between the LPS and CBD+LPS groups.
In this study, we confirmed the homeostatic expression of GPR55 in HGFs and its tendency to decrease in expression during inflammation. It was also shown that part of an anti-inflammatory effect by CBD may be mediated by GPR55.
"},{"@language":"ja","@value":"大麻に含まれるカンナビジオール (CBD) は抗炎症作用を有し, その受容体とされるG-protein coupled receptor 55 (GPR55) は炎症制御との関連が示唆されている. 本論文ではヒト歯肉線維芽細胞 (HGFs) におけるGPR55の発現状態と, GPR55を介したCBDの抗炎症作用とを検討した. HGFsをControl群, LPS群, CBD処理後LPS刺激群 (CBD＋LPS群), CBD群に分け受容体の発現および局在を確認した. GPR55ノックダウンの有無により, 培養上清中のInterleukin (IL) -6およびIL-8タンパク質産生量を比較した. GPR55の発現量はLPS群およびCBD＋LPS群で有意に減少し (p<0.05), タンパク質の局在は全群のHGFsの細胞質内にびまん性に認められた. また, 非ノックダウンHGFsではLPS群と比較してCBD＋LPS群で有意にIL-6, 8の産生が減少していた (p<0.05). 一方ノックダウンHGFsでは, control群と比較してLPS群とCBD＋LPS群で有意にIL-6, 8の産生が増加していたが (p<0.05), 両群間に有意な差はなかった. 本研究ではHGFsにおけるGPR55の恒常的発現と, 炎症下での発現減少傾向を確認し, CBDによる抗炎症作用の一部がGPR55を介する可能性が示唆された.
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Jpn. Soc. Periodontol."},{"@language":"ja","@value":"日歯周誌"},{"@language":"en","@value":"J Jpn Soc Periodontol"}],"dc:publisher":[{"@language":"en","@value":"JAPANESE SOCIETY OF PERIODONTOLOGY"},{"@language":"ja","@value":"特定非営利活動法人 日本歯周病学会"}],"prism:publicationDate":"2021-03-31","prism:volume":"63","prism:number":"1","prism:startingPage":"11","prism:endingPage":"23"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"https://www.jstage.jst.go.jp/article/perio/63/1/63_11/_pdf"},{"@id":"https://search.jamas.or.jp/link/ui/2021223079"}],"availableAt":"2021-03-31","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Cannabidiol","dc:title":"Cannabidiol"},{"@id":"https://cir.nii.ac.jp/all?q=G%20protein-coupled%20receptor%2055","dc:title":"G protein-coupled receptor 55"},{"@id":"https://cir.nii.ac.jp/all?q=Anti-inflammatory%20effects","dc:title":"Anti-inflammatory effects"},{"@id":"https://cir.nii.ac.jp/all?q=Cytokines","dc:title":"Cytokines"},{"@id":"https://cir.nii.ac.jp/all?q=Human%20gingival%20fibroblasts","dc:title":"Human gingival fibroblasts"},{"@id":"https://cir.nii.ac.jp/all?q=%E3%82%AB%E3%83%B3%E3%83%8A%E3%83%93%E3%82%B8%E3%82%AA%E3%83%BC%E3%83%AB","dc:title":"カンナビジオール"},{"@id":"https://cir.nii.ac.jp/all?q=G%E3%82%BF%E3%83%B3%E3%83%91%E3%82%AF%E8%B3%AA%E5%85%B1%E5%BD%B9%E5%9E%8B%E5%8F%97%E5%AE%B9%E4%BD%93GPR55","dc:title":"Gタンパク質共役型受容体GPR55"},{"@id":"https://cir.nii.ac.jp/all?q=%E6%8A%97%E7%82%8E%E7%97%87%E4%BD%9C%E7%94%A8","dc:title":"抗炎症作用"},{"@id":"https://cir.nii.ac.jp/all?q=%E3%82%B5%E3%82%A4%E3%83%88%E3%82%AB%E3%82%A4%E3%83%B3","dc:title":"サイトカイン"},{"@id":"https://cir.nii.ac.jp/all?q=%E3%83%92%E3%83%88%E6%AD%AF%E8%82%89%E7%B7%9A%E7%B6%AD%E8%8A%BD%E7%B4%B0%E8%83%9E","dc:title":"ヒト歯肉線維芽細胞"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050001339049028992","@type":"Article","resourceType":"学術雑誌論文(journal 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