Molecular Confirmation of an Indole-Negative <i>Klebsiella oxytoca</i> Isolated from a Patient with Cystitis
-
- Takano Youta
- Department of Clinical Laboratory, Nagano Chuo Hospital, Japan
-
- Shibano Makiko
- Department of Clinical Laboratory, Nagano Chuo Hospital, Japan
-
- Takizawa Yuuya
- Department of Clinical Laboratory, Nagano Chuo Hospital, Japan
-
- Arai Eriko
- Department of Laboratory Medicine, Shinshu University Hospital, Japan
-
- Koyama Shinobu
- MIROKU Medical Laboratory Inc., Japan
-
- Shimizu Takahiro
- Department of Clinical Laboratory, Nagano Municipal Hospital, Japan
-
- Matsumoto Takehisa
- Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, Japan
Bibliographic Information
- Other Title
-
- Molecular Confirmation of an Indole-Negative Klebsiella oxytoca Isolated from a Patient with Cystitis
Search this article
Description
<p>Klebsiella oxytoca is an opportunistic pathogen that causes nosocomial infections. Here, we describe an unusual clinical strain of indole-negative K. oxytoca, GU175, isolated from the urine of a patient with cystitis. The GU175 strain was identified as K. pneumoniae with a probability of 99.40%, negative for indole production, and resistant to third-generation cephalosporins by using the MicroScan Walkaway 40 SI system with the Negative combo EN1 J panel. Biochemical characterization of this strain using lysine-indole motility medium was negative for indole production. However, identification tests using the MALDI Biotyper system and 16S rRNA gene sequence analysis revealed that GU175 is K. oxytoca. DNA sequence analysis of the tryptophanase operon comparing the GU175 strain with the revertant GU176 strain, which tested positive for indole, revealed a point mutation in the Shine-Dalgarno sequence upstream of tnaC in the GU175 strain. This is the first report of indole-negative K. oxytoca, which was attributed to a mutation in the DNA sequence of the tryptophanase operon isolated from a patient with a urinary tract infection. As indole-negative K. oxytoca can be misidentified as K. pneumoniae by biochemical characterization, clinical microbiologists should be aware of such misidentifications.</p>
Journal
-
- Japanese Journal of Infectious Diseases
-
Japanese Journal of Infectious Diseases 76 (2), 159-161, 2023-03-31
National Institute of Infectious Diseases
- Tweet
Details 詳細情報について
-
- CRID
- 1390295524492372224
-
- NII Book ID
- AA1132885X
-
- ISSN
- 18842836
- 13446304
-
- NDL BIB ID
- 032759595
-
- PubMed
- 36575027
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
-
- Abstract License Flag
- Disallowed