To evaluate the usefulness of 2-methacryloyloxyethyl phosphorylcholine polymer (MPCP) as a gargle, we investigated the bacterial adherence inhibitory effect, moisturizing effect, and anti-inflammatory effect of MPCP37, one of the formulations of MPCP. In an in vitro experiment we counted the numbers of Streptococcus pneumoniae adhering to cultured pharyngeal epithelial cells after exposing the cells to MPCP37. We then counted the numbers of bacteria adhering to the mucosal epithelial cells of 15 healthy subjects three hours after gargling with MPCP37 and various commercially available gargles. The moisturizing effect of MPCP37 was assessed by measuring the humidity of the tongue surface with an oral moisture meter after gargling with MPCP37 and other gargles and absorbing tongue surface water with a paper towel. The anti-inflammatory effect of MPCP37 was assessed by measuring the levels of IL-8 produced by cultured pharyngeal epithelial cells in response to the stimulation with Pam3CSK4 after exposure to MPCP37. The results showed that the numbers of bacteria adhering to the epithelial cells had markedly decreased after exposure to MPCP37 and gargling with MPCP37, even with a 0.05% MPCP37 solution. The change in tongue surface moisture was significantly smaller in the group that had gargled with MPCP37. In addition, IL-8 production by cultured pharyngeal epithelial cells was significantly suppressed by exposure to MPCP37. These findings suggested that MPCP37 possesses moisturizing and anti-inflammatory effects in addition to an inhibitory effect on bacterial adherence and that it might be able to serve as a formulation of a new gargle.
"},{"@language":"ja","@value":"2-メタクリロイルオキシエチルホスホリルコリンポリマー (MPCP) の含嗽薬としての有用性を評価するため, その製剤の一つである MPCP37 の上皮細胞への細菌付着抑制作用, 保湿作用, 抗炎症作用を検討した.
まず, 培養咽頭上皮細胞を MPCP37 で処理したのちに肺炎球菌を添加し, 上皮細胞への付着細菌数を測定した. さらに, 15名の健常者を対象として MPCP37 および市販されている各種含嗽薬で含嗽を行い, その3時間後の頬粘膜擦過上皮細胞への付着細菌数を測定した. 保湿作用は, MPCP37 および各種含嗽薬で含嗽したあとにペーパータオルで舌表面の水分を吸収し, その前後での舌表面の乾燥状態を口腔水分計で計測し, 両者の差を比較し評価した. 抗炎症作用は, 培養咽頭癌上皮細胞を MPCP37 および各種含嗽薬で処理したのちに Pam3CSK4 で刺激し, 培養液中の IL-8 の値を測定し評価した.
その結果, MPCP37 の前処理および含嗽によって上皮細胞への付着細菌数が有意に減少し, 0.05%溶液でも細菌付着の抑制作用が認められた. また, 舌表面水分量の変化は MPCP37 含嗽群で有意に少なく, 上皮細胞の IL-8 産生量は MPCP37 の前処理によって有意に減少した.
これらの結果から, MPCP37 は細菌付着抑制作用に加えて保湿作用さらに抗炎症作用を有し, 新たな含嗽薬として応用できる製剤であることが示唆された.
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Masaru"},{"@language":"ja","@value":"山下 勝"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"鹿児島大学大学院医歯学総合研究科耳鼻咽喉科・頭頸部外科学分野"},{"@language":"en","@value":"Department of Otolaryngology, Head and Neck surgery, Kagoshima University Graduate School of Medical and Dental Sciences"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"24365793"},{"@type":"EISSN","@value":"24365866"},{"@type":"NDL_BIB_ID","@value":"031556723"},{"@type":"ISSN","@value":"24365793"},{"@type":"LISSN","@value":"24365793"},{"@type":"NCID","@value":"AA1292408X"}],"prism:publicationName":[{"@language":"en","@value":"Nippon Jibiinkoka Tokeibugeka Gakkai Kaiho(Tokyo)"},{"@language":"ja","@value":"日本耳鼻咽喉科頭頸部外科学会会報"},{"@language":"ja","@value":"日耳鼻"}],"dc:publisher":[{"@language":"en","@value":"Japanese Society of Otorhinolaryngology-Head and Neck Surgery"},{"@language":"ja","@value":"一般社団法人 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