Mortalities due to Lactococcus garvieae-like infection have been observed in farmed greater amberjack Seriola dumerili previously immunized with a commercial L. garvieae vaccine containing serotype I and II strains in Miyazaki Prefecture since August 2021. The isolates from diseased fish were not agglutinated by the anti-serotype I and II sera, and showed genetic similarity to L. garvieae serotype I in multi-locus sequence (16S rRNA gene, pheS, recA, rpoA and rpoB) analysis. The pathogenicity of isolates was confirmed by intraperitoneal injection in greater amberjack, and the lethal dose fifty was less than 1.6 × 103 CFU/FISH. In a vaccine trial, greater amberjack, which had been immunized with formalin-killed cells (FKC) prepared from both serotype I and II strains, was not effectively protected against an isolate from this study, whereas those immunized with FKC prepared using the isolate were effectively protected against the infection with an isolate exhibiting the same antigenicity. Based on these findings, we propose a new serotype: serotype III, which is not agglutinated with both anti-serotype I and II serum and not protected against a serotype I and serotype II-combined vaccine.
"},{"@language":"ja","@value":"Lactococcus garvieaeⅠ型およびⅡ型を含む市販の混合ワクチンを投与した宮崎県の養殖カンパチで，2021年8月からL. garvieae感染症に類似した症状を呈する疾病が確認されるようになった。分離された菌株は，L. garvieae診断用抗Ⅰ型およびⅡ型血清に対して非凝集性を示し，多遺伝子座配列解析（MLSA）においてL. garvieaeⅠ型に近く，Ⅱ型とは遺伝学的相違性が認められた。同菌株を用いたカンパチに対する腹腔内接種による感染試験では，半数致死濃度は 1.6×103 CFU/FISH未満であった。また，Ⅰ型およびⅡ型の混合ワクチンを接種したカンパチにおいて，今回分離した菌株に対する感染防御効果は認められなかった。一方，今回分離した菌株のホルマリン不活化ワクチンは，同菌株の感染に対して高い防御効果が確認された。以上の結果から，今回分離された従来のⅠ型およびⅡ型診断用抗血清に凝集せず，Ⅰ型およびⅡ型ワクチンの効果がない菌株をL. garvieaeⅢ型と呼称することを提案する。
"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410298742739269506","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Minami Takayuki"},{"@language":"ja","@value":"南 隆之"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"宮崎県水産試験場"},{"@language":"en","@value":"Miyazaki Prefectural Fisheries Research Institute"}]},{"@id":"https://cir.nii.ac.jp/crid/1410298742739269504","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Yoshida Terutoyo"},{"@language":"ja","@value":"吉田 照豊"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"宮崎大学農学部"},{"@language":"en","@value":"Faculty of Agriculture, University of Miyazaki"}]},{"@id":"https://cir.nii.ac.jp/crid/1410298742739269505","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Nishiki Issei"},{"@language":"ja","@value":"西木 一生"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"宮崎大学農学部"},{"@language":"en","@value":"Faculty of Agriculture, University of Miyazaki"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"0388788X"},{"@type":"EISSN","@value":"18817335"},{"@type":"ISSN","@value":"0388788X"},{"@type":"NDL_BIB_ID","@value":"000000025845"},{"@type":"LISSN","@value":"0388788X"},{"@type":"NCID","@value":"AN00063165"}],"prism:publicationName":[{"@language":"en","@value":"Fish Pathology"},{"@language":"ja","@value":"魚病研究"},{"@language":"en","@value":"Fish Pathol."},{"@language":"en","@value":"Gyobyo Kenkyu"},{"@language":"ja","@value":"魚病研究"},{"@language":"en","@value":"Fish Pathology"}],"dc:publisher":[{"@language":"en","@value":"The Japanese Society of Fish Pathology"},{"@language":"ja","@value":"日本魚病学会"}],"prism:publicationDate":"2023-12-15","prism:volume":"58","prism:number":"4","prism:startingPage":"146","prism:endingPage":"152"},"reviewed":"false","url":[{"@id":"https://agriknowledge.affrc.go.jp/RN/2010950467"},{"@id":"http://id.ndl.go.jp/bib/033268299"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I033268299"},{"@id":"https://www.jstage.jst.go.jp/article/jsfp/58/4/58_146/_pdf"}],"availableAt":"2023-12-15","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=%3Ci%3ESeriola%20dumerili%3C/i%3E","dc:title":"Seriola dumerili"},{"@id":"https://cir.nii.ac.jp/all?q=%3Ci%3ELactococcus%20garvieae%3C/i%3E","dc:title":"Lactococcus garvieae"},{"@id":"https://cir.nii.ac.jp/all?q=non-agglutinating","dc:title":"non-agglutinating"},{"@id":"https://cir.nii.ac.jp/all?q=serotype","dc:title":"serotype"},{"@id":"https://cir.nii.ac.jp/all?q=pathogenicity","dc:title":"pathogenicity"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050585039690267776","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Cell surface properties of Lactococcus garvieae strains and their immunogenicity in yellowtail, Seriola quinqueradiata."},{"@value":"Cell-surface properties of Lactococcus garvieae strains and their immunogenicity in the yellowtail Seriola quinqueradiata"}]},{"@id":"https://cir.nii.ac.jp/crid/1360017674236115584","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Proposal of Lactococcus garvieae subsp. bovis Varsha and Nampoothiri 2016 as a later heterotypic synonym of Lactococcus formosensis Chen et 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