Genetic analysis of the two fused chromosomes derived from the sex-limited strain TWPB of the silkworm, <i>Bombyx mori</i>

DOI
  • Fujii Tsuguru
    Laboratory of Silkworm Genetic Resources, Institute of Genetic Resources, Kyushu University Graduate School of BioResources and Bioenvironmental Science
  • Seigo Kuwazaki
    National Institute of Agrobiological Science
  • Abe Hiroaki
    Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • Fujimoto Toshiaki
    Laboratory of Silkworm Genetic Resources, Institute of Genetic Resources, Kyushu University Graduate School of BioResources and Bioenvironmental Science
  • Hino Masato
    Laboratory of Silkworm Genetic Resources, Institute of Genetic Resources, Kyushu University Graduate School of BioResources and Bioenvironmental Science
  • Yamamoto Kimiko
    National Institute of Agrobiological Science
  • Shimada Toru
    Department of Life Science, Faculty of Science, Gakushuin University

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Description

<p> The silkworm Bombyx mori TWPB strain has a large chromosome formed by the fusion of the W chromosome with a fragment of chromosome 2 and chromosome 5. The chromosome 2 region of chromosome W-II-V has pB mutation which causes the larval integument to be black. Larvae with exceptional phenotypes—such as black (pB) and moricaud (pM) males that are presumably generated by fission event occurring in chromosome W-II-V—occasionally occur during the maintenance of the TWPB strain. In the pM male progenies, segregated in the TWPB strain, fragment consisting of chromosome 2 and 5 dissociate from W-II-V chromosome and fuse with chromosomes Z and 13, generating two new fused chromosomes Z-II-V and XIII-II-V. Using bacterial artificial chromosome clones and fluorescent in situ hybridization (BAC-FISH mapping), we analyzed the structure of the fused chromosomes. We also analyzed their structures using the recombination values between pM and phenotypic markers in the chromosomes Z and 13. Further, using a PCR-based molecular marker, we confirmed that no protein-coding genes on chromosomes Z and 13 were lost due to the fusion events. Moreover, we found through the crossing experiments with Z-II-V strain that the fusion resulted in the formation of neo-Z/W neo-W sex chromosome constitution. The TWPB strain and the TWPB-derived-novel-fusion-carrying strains are useful for the genetic and molecular cytogenic analysis of the chromosomal rearrangements in lepidopterans.</p>

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Details 詳細情報について

  • CRID
    1390303932784138368
  • DOI
    10.11416/jibs.93.3_023
  • ISSN
    18847978
    13468073
  • Text Lang
    en
  • Data Source
    • JaLC
  • Abstract License Flag
    Disallowed

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