Detection of CCND1 gene copy number variations using multiplex ligation-dependent probe amplification and fluorescence in situ hybridization methods
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金沢大学医薬保健研究域医学系
The CCND1 locus is located in 11q13 and encodes the G1–S regulatory protein, cyclin D1. Cyclin D1 is frequently amplified in various types of cancers, and is an attractive potential therapeutic target. Multiplex ligation-dependent probe amplification (MLPA) is a new, high-resolution method for the detection of amplification of numerous genes including CCND1 in small amounts of DNA fragments derived from formalin-fixed, paraffin-embedded material in a single reaction. This approach is, however, based on PCR and averages many different cells, so validation by morphological methods such as fluorescence in situ hybridization (FISH) is theoretically mandatory. Here we describe detection of CCND1 gene copy number variations by commercially available MLPA kits and FISH using a bacterial artificial chromosome (BAC) probe. © Springer Science+Business Media, LLC 2018.
Embargo Period 12 months
収録刊行物
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- Methods in Molecular Biology
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Methods in Molecular Biology 1726 101-109, 2018-02-22
Humana Press Inc. / Springer
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詳細情報 詳細情報について
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- CRID
- 1390572174913913984
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- NII論文ID
- 120006454998
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- NII書誌ID
- AA11067778
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- ISSN
- 10643745
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- Web Site
- http://hdl.handle.net/2297/00050498
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- CiNii Articles