{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390573715160834176.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.3800/pbr.17.127"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"032186260"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/032186260"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I032186260"}},{"identifier":{"@type":"URI","@value":"https://www.jstage.jst.go.jp/article/pbr/17/2/17_B170204/_pdf"}}],"dc:title":[{"@language":"en","@value":"Assessment of spawning events of the tri-spine horseshoe crab, <i>Tachypleus tridentatus</i>, using environmental DNA in the Sone tidal flat, Japan"},{"@value":"Assessment of spawning events of the tri-spine horseshoe crab, Tachypleus tridentatus, using environmental DNA in the Sone tidal flat, Japan"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"<p>The conservation of an endangered horseshoe crab, <i>Tachypleus tridentatus</i>, has been increasingly emphasized worldwide. Although monitoring of <i>T. tridentatus</i> reproduction is effective for its conservation, the conventional monitoring system for spawning has several drawbacks. Thus, our study aimed to examine the environmental DNA (i.e., eDNA) approach to assess <i>T. tridentatus</i> spawning events. Six survey sites were set at the sandy beaches of the Sone tidal flat, Japan. Visual observations of the spawning events, i.e., the presence/absence of adult pairs of <i>T. tridentatus</i> or that of foams, occurring because of their spawning behavior, and water sampling for eDNA assay were conducted 10 times at each site between 2019 and 2020. The spawning events were observed at a total 16 sites during the surveys, and the estimated numbers of <i>T. tridentatus</i> adult pairs were 1–5 per site. <i>T. tridentatus</i> DNA was detected in 15 of the 16 samples at the sites where the spawning events were observed and in 13 of 44 samples where the spawning events were not observed. The estimated number of pairs and the eDNA concentration showed a significantly positive correlation. Moreover, the presence/absence of the spawning events was distinguished with excellent accuracy based on the eDNA concentration. Therefore, our results showed that the spawning events of the species and the eDNA concentration were closely related. In conclusion, we expect that the population of <i>T. tridentatus</i> in spawning grounds can be evaluated more complementarily by combining the conventional observation method with the eDNA approach.</p>"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420845751163438208","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"50814662"},{"@type":"NRID","@value":"1000050814662"},{"@type":"ORCID","@value":"0000-0002-7037-1208"},{"@type":"NRID","@value":"9000312730624"},{"@type":"NRID","@value":"9000405666811"},{"@type":"NRID","@value":"9000342792043"},{"@type":"NRID","@value":"9000356621623"},{"@type":"NRID","@value":"9000398785099"},{"@type":"NRID","@value":"9000398962517"},{"@type":"NRID","@value":"9000381065669"},{"@type":"NRID","@value":"9000398793645"},{"@type":"NRID","@value":"9000323830154"},{"@type":"NRID","@value":"9000410756530"},{"@type":"NRID","@value":"9000410901379"},{"@type":"NRID","@value":"9000405666930"},{"@type":"NRID","@value":"9000242666586"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/koyama_akihiko"}],"foaf:name":[{"@language":"en","@value":"Koyama Akihiko"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Faculty of Advanced Science and Technology, Kumamoto University"},{"@language":"en","@value":"Fishery Research Laboratory, Kyushu University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410573715160834178","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Kawahara Yuki"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Civil and Environmental Engineering, Kumamoto University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410573715160834180","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Kurita Yoshihisa"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Fishery Research Laboratory, Kyushu University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410573715160834184","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Hirata Taiga"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Civil and Environmental Engineering, Kumamoto University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410573715160834179","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Hayashi Osamu"}],"jpcoar:affiliationName":[{"@language":"en","@value":"The Japanese Society for the Preservation of the Horseshoe crab (Nihon Kabutogani wo Mamoru Kai)"}]},{"@id":"https://cir.nii.ac.jp/crid/1410573715160834177","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Takahashi Shungo"}],"jpcoar:affiliationName":[{"@language":"en","@value":"The Japanese Society for the Preservation of the Horseshoe crab (Nihon Kabutogani wo Mamoru Kai)"}]},{"@id":"https://cir.nii.ac.jp/crid/1410573715160834176","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Kubozono Haruka"}],"jpcoar:affiliationName":[{"@language":"en","@value":"The 21st Century Program, Kyushu University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410573715160834182","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Onikura Norio"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Fishery Research Laboratory, Kyushu University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410573715160834183","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Minagawa Tomoko"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Faculty of Advanced Science and Technology, Kumamoto University"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"18808247"},{"@type":"LISSN","@value":"18808247"},{"@type":"EISSN","@value":"1882627X"},{"@type":"NDL_BIB_ID","@value":"000008045714"},{"@type":"ISSN","@value":"18808247"},{"@type":"NCID","@value":"AA12130745"}],"prism:publicationName":[{"@language":"en","@value":"Plankton and Benthos Research"},{"@language":"en","@value":"Plankton Benthos Res"}],"dc:publisher":[{"@language":"en","@value":"The Plankton Society of Japan, The Japanese Association of Benthology"},{"@language":"ja","@value":"日本プランクトン学会、日本ベントス学会"}],"prism:publicationDate":"2022-05-30","prism:volume":"17","prism:number":"2","prism:startingPage":"127","prism:endingPage":"136"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://id.ndl.go.jp/bib/032186260"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I032186260"},{"@id":"https://www.jstage.jst.go.jp/article/pbr/17/2/17_B170204/_pdf"}],"availableAt":"2022-05-30","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Citizen%20science","dc:title":"Citizen science"},{"@id":"https://cir.nii.ac.jp/all?q=Intertidal%20zone","dc:title":"Intertidal zone"},{"@id":"https://cir.nii.ac.jp/all?q=Reproduction","dc:title":"Reproduction"},{"@id":"https://cir.nii.ac.jp/all?q=Sandy%20beach","dc:title":"Sandy beach"},{"@id":"https://cir.nii.ac.jp/all?q=Tri-spine%20horseshoe%20crab","dc:title":"Tri-spine horseshoe crab"},{"@id":"https://cir.nii.ac.jp/all?q=Citizen%20science","dc:title":"Citizen science"},{"@id":"https://cir.nii.ac.jp/all?q=Intertidal%20zone","dc:title":"Intertidal zone"},{"@id":"https://cir.nii.ac.jp/all?q=Reproduction","dc:title":"Reproduction"},{"@id":"https://cir.nii.ac.jp/all?q=Sandy%20beach","dc:title":"Sandy beach"},{"@id":"https://cir.nii.ac.jp/all?q=Tri-spine%20horseshoe%20crab","dc:title":"Tri-spine horseshoe crab"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050012570391595776","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"An illustrated manual for environmental DNA research: Water sampling guidelines and experimental protocols"}]},{"@id":"https://cir.nii.ac.jp/crid/1050580007680447488","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Habitat suitability maps for juvenile 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Opportunities"}]},{"@id":"https://cir.nii.ac.jp/crid/1360579818443217920","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Citizen science frontiers horseshoe crab population regain at their spawning beach in East Peninsular Malaysia"}]},{"@id":"https://cir.nii.ac.jp/crid/1360579819669278336","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Public Awareness and Community-Based Conservation for the Horseshoe Crab at Saikai National Park in Nagasaki Prefecture, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1360861291551255040","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Spatial and temporal distribution of horseshoe crab (Limulus polyphemus) spawning in Delaware Bay: Implications for monitoring"}]},{"@id":"https://cir.nii.ac.jp/crid/1360861292446198656","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Future of Asian horseshoe crab conservation under 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DNA"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418521244402688","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Can environmental DNA (eDNA) be used for detection and monitoring of introduced crab species in the Baltic Sea?"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418521360302080","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Conservation in a cup of water: estimating biodiversity and population abundance from environmental DNA"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699994772608640","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Present and Potential Future Distributions of Asian Horseshoe Crabs Determine Areas for Conservation"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262943747471360","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Environmental<scp>DNA</scp>surveillance for invertebrate species: advantages and technical limitations to 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