{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390575495614927616.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.15583/jpchrom.2022.011"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"032504211"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/032504211"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I032504211"}},{"identifier":{"@type":"URI","@value":"https://www.jstage.jst.go.jp/article/jpchrom/43/3/43_2022.011/_pdf"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Two-Dimensional Chiral HPLC Analysis of Lactate, Hydroxybutyrates and Malate in Human Plasma"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"<p>For the simultaneous determination of lactate (LA), 2-hydroxybutyrate (2HB), 3-hydroxybutyrate (3HB) and malate (MA) enantiomers, a new separation technique in a two-dimensional high-performance liquid chromatography (2D-HPLC) system following the fluorescence derivatization with 4-nitro-7-piperazino-2,1,3-benzoxadiazole has been developed. The 2D-HPLC system was composed of the reversed-phase (1D) and enantioselective (2D) separations. In the first dimension, the target hydroxy acids were separated as their D plus L mixtures on a Singularity RP18 column (1.0 mm i.d. x 250 mm), and the enantiomers were separated in the second dimension using a polysaccharide-based chiral stationary phase, Chiralpak IG (2.0 mm i.d. x 250 mm). By using the Chiralpak IG column, the LA, 2HB, 3HB and MA enantiomers were separated with resolution values of 3.47, 3.63, 6.81 and 3.72, respectively. The developed 2D-HPLC system was applied to human plasma, and trace levels of D-LA and L-3HB in addition to their major antipodes were determined. The acquired %D of LA (the percentage of D-LA over total LA) and %L of 3HB (the percentage of L-3HB over total 3HB) were 1.0 and 2.7, respectively. For 2HB, only the L-form was detected, while the MA enantiomers were not found in the human plasma.</p>"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410575495614927619","@type":"Researcher","foaf:name":[{"@language":"en","@value":"TSAI Hsin-Miao"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of Pharmaceutical Sciences, Kyushu University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410575495614927616","@type":"Researcher","foaf:name":[{"@language":"en","@value":"HSIEH Chin-Ling"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of Pharmaceutical Sciences, Kyushu University"}]},{"@id":"https://cir.nii.ac.jp/crid/1420005899165985536","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"90905308"},{"@type":"NRID","@value":"1000090905308"}],"foaf:name":[{"@language":"en","@value":"ISHII Chiharu"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of Pharmaceutical Sciences, Kyushu University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410575495614927622","@type":"Researcher","foaf:name":[{"@language":"en","@value":"AKITA Takeyuki"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of Pharmaceutical Sciences, Kyushu University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410575495614927621","@type":"Researcher","foaf:name":[{"@language":"en","@value":"MITA Masashi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"KAGAMI, Inc."}]},{"@id":"https://cir.nii.ac.jp/crid/1410575495614927617","@type":"Researcher","foaf:name":[{"@language":"en","@value":"IDE Tomomi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of Medical Sciences, Kyushu University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410575495614927618","@type":"Researcher","foaf:name":[{"@language":"en","@value":"LEE Jen-Ai"}],"jpcoar:affiliationName":[{"@language":"en","@value":"School of Pharmacy, Taipei Medical University"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801193673728","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"10284522"},{"@type":"NRID","@value":"1000010284522"},{"@type":"NRID","@value":"9000004506523"},{"@type":"NRID","@value":"9000392141565"},{"@type":"NRID","@value":"9000259859655"},{"@type":"NRID","@value":"9000317158684"},{"@type":"NRID","@value":"9000414928147"},{"@type":"NRID","@value":"9000414251524"},{"@type":"NRID","@value":"9000408424595"},{"@type":"NRID","@value":"9000396146043"},{"@type":"NRID","@value":"9000400172828"},{"@type":"NRID","@value":"9000324995204"},{"@type":"NRID","@value":"9000410626621"}],"foaf:name":[{"@language":"en","@value":"HAMASE Kenji"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Graduate School of Pharmaceutical Sciences, Kyushu University"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13428284"},{"@type":"LISSN","@value":"13428284"},{"@type":"EISSN","@value":"13483315"},{"@type":"NDL_BIB_ID","@value":"000000101366"},{"@type":"ISSN","@value":"13428284"},{"@type":"NCID","@value":"AA1137755X"}],"prism:publicationName":[{"@language":"en","@value":"CHROMATOGRAPHY"},{"@language":"ja","@value":"CHROMATOGRAPHY"}],"dc:publisher":[{"@language":"en","@value":"The Society for Chromatographic Sciences"},{"@language":"ja","@value":"クロマトグラフィー科学会"}],"prism:publicationDate":"2022-10-20","prism:volume":"43","prism:number":"3","prism:startingPage":"119","prism:endingPage":"126"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://id.ndl.go.jp/bib/032504211"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I032504211"},{"@id":"https://www.jstage.jst.go.jp/article/jpchrom/43/3/43_2022.011/_pdf"}],"availableAt":"2022-10-20","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Enantioselective%20separation","dc:title":"Enantioselective separation"},{"@id":"https://cir.nii.ac.jp/all?q=Lactate","dc:title":"Lactate"},{"@id":"https://cir.nii.ac.jp/all?q=Hydroxybutyrate","dc:title":"Hydroxybutyrate"},{"@id":"https://cir.nii.ac.jp/all?q=Malate","dc:title":"Malate"},{"@id":"https://cir.nii.ac.jp/all?q=Two-dimensional%20HPLC","dc:title":"Two-dimensional 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1-(9-fluorenyl)ethyl chloroformate"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262945897367168","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"3‐Hydroxybutyrate regulates energy metabolism and induces <scp>BDNF</scp> expression in cerebral cortical neurons"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544418629982464","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Establishment of a two-dimensional chiral HPLC system for the simultaneous detection of lactate and 3-hydroxybutyrate enantiomers in human clinical samples"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419199328896","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Simultaneous determination of l- and d-lactic acid in plasma by capillary electrophoresis"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825895271817728","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Contents of D-lactate and its related 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