Fungi uniquely synthesize lysine through the α-aminoadipate pathway. The saccharopine reductase ScLys9 catalyzes the formation of saccharopine from ɑ-aminoadipate 6-semialdehyde, the seventh step in the lysine biosynthesis pathway in Saccharomyces cerevisiae. Here, we characterized the functions of TrLys9, an ortholog of S. cerevisiae ScLys9 in the industrial filamentous fungus Trichoderma reesei. Transcriptional level analysis indicated that TrLYS9 expression was higher in the conidial stage than in other stages. Disruption of TrLYS9 led to lysine auxotrophy. Phenotype analysis of the ΔTrlys9 mutant showed that TrLYS9 was involved in fungal development including vegetative growth, conidiation, and conidial germination and lysine biosynthesis. Cellulase production was also impaired in the ΔTrlys9 mutant due to the failure of conidial germination in liquid cellulase-inducing medium. Defects in radial growth and asexual development of the ΔTrlys9 mutant were fully recovered when exogenous lysine was added to the medium. These results imply that TrLys9 is involved in fungal development and lysine biosynthesis in T. reesei.
"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410579830890111488","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Lan Jinling"}],"jpcoar:affiliationName":[{"@language":"en","@value":"College of Plant Protection, Jilin Agricultural University"},{"@language":"en","@value":"Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences"},{"@language":"en","@value":"National Center of Technology Innovation for Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences"}]},{"@id":"https://cir.nii.ac.jp/crid/1410579830890111490","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Zhang Lin"}],"jpcoar:affiliationName":[{"@language":"en","@value":"College of Plant Protection, Jilin Agricultural University"},{"@language":"en","@value":"Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences"},{"@language":"en","@value":"National Center of Technology Innovation for Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences"}]},{"@id":"https://cir.nii.ac.jp/crid/1410579830890111491","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Gao Jie"}],"jpcoar:affiliationName":[{"@language":"en","@value":"College of Plant Protection, Jilin Agricultural University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410579830890111489","@type":"Researcher","foaf:name":[{"@language":"en","@value":"He Ronglin"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences"},{"@language":"en","@value":"National Center of Technology Innovation for Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00221260"},{"@type":"LISSN","@value":"00221260"},{"@type":"EISSN","@value":"13498037"}],"prism:publicationName":[{"@language":"en","@value":"The Journal of General and Applied Microbiology"},{"@language":"en","@value":"J. Gen. Appl. Microbiol."}],"dc:publisher":[{"@language":"en","@value":"Applied Microbiology, Molecular and Cellular Biosciences Research Foundation"},{"@language":"ja","@value":"公益財団法人 応用微生物学・分子細胞生物学研究奨励会"}],"prism:publicationDate":"2023","prism:volume":"69","prism:number":"3","prism:startingPage":"159","prism:endingPage":"166"},"reviewed":"false","url":[{"@id":"https://www.jstage.jst.go.jp/article/jgam/69/3/69_2023.01.002/_pdf"}],"availableAt":"2023","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=lysine%20biosynthesis","dc:title":"lysine biosynthesis"},{"@id":"https://cir.nii.ac.jp/all?q=%3Ci%3ETrichoderma%20reesei%3C/i%3E","dc:title":"Trichoderma reesei"},{"@id":"https://cir.nii.ac.jp/all?q=vegetative%20growth","dc:title":"vegetative growth"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360011143491075584","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Involvement of aMagnaporthe griseaSerine/Threonine Kinase Gene, MgATG1, in Appressorium Turgor and 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