In our previous study, we found that the peaks of chlorophyll fluorescence spectra of K. mikimotoi and Chattonella spp. shift toward higher wavelengths, defined the degree of peak shifts as the fluorescence spectral shift index (FSI), and developed a method for detecting these harmful algal species and an in situ device, the harmful algal indication sensor (HAI sensor). In this study, we further established a method for estimating the cell abundance of harmful algal species based on FSI, and validated the method through laboratory experiments with cultured strains. Furthermore, a series of field observations for K. mikimotoi was conducted in Saiki Bay, Oita, in 2017 and 2018, and in Kujukushima waters, Nagasaki, in 2019 and 2021. Based on the results from observations, the detection method and the cell abundance estimation method were evaluated. High true positive fractions were confirmed in both methods. Significant positive correlations on a logarithmic scale were observed between the estimated cell densities and cell densities counted by microscopy.
"},{"@language":"ja","@value":"我々はこれまでに有害赤潮藻Karenia mikimotoiのクロロフィル蛍光スペクトルのピーク波長が他種より僅かに移動していること，さらにその移動の程度を表す指標FSI（Fluorescence spectral Shift Index）を用いた有害赤潮藻の存在検知法の有効性を報告した。本研究では，新たに考案した細胞密度推定法を現場観測データに適用した結果，K. mikimotoi細胞の存在を高い真陽性率で検出判定し，推定密度と検鏡密度の対数値間に有意な正相関が観察された。
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Fisheries"}]},{"@id":"https://cir.nii.ac.jp/crid/1410584565601035649","@type":"Researcher","foaf:name":[{"@language":"en","@value":"NAKAJIMA YOSHIHIRO"},{"@language":"ja","@value":"中島 吉洋"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Fisheries Division, Nagasaki Prefectural Government"},{"@language":"ja","@value":"長崎県水産部"}]},{"@id":"https://cir.nii.ac.jp/crid/1410584565601035659","@type":"Researcher","foaf:name":[{"@language":"en","@value":"MUKAI KOKI"},{"@language":"ja","@value":"向井 幸樹"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Faculty of Agriculture, Kyushu University"},{"@language":"ja","@value":"九州大学大学院農学研究院"}]},{"@id":"https://cir.nii.ac.jp/crid/1410584565601035651","@type":"Researcher","foaf:name":[{"@language":"en","@value":"KATO HIROHARU"},{"@language":"ja","@value":"加藤 宏晴"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"JFEアドバンテック株式会社"},{"@language":"en","@value":"JFE Advantech Co., Ltd."}]},{"@id":"https://cir.nii.ac.jp/crid/1410584565601035652","@type":"Researcher","foaf:name":[{"@language":"en","@value":"HONDA SEIICHIRO"},{"@language":"ja","@value":"本田 清一朗"}],"jpcoar:affiliationName":[{"@language":"en","@value":"JFE Advantech Co., Ltd."},{"@language":"ja","@value":"JFEアドバンテック株式会社"}]},{"@id":"https://cir.nii.ac.jp/crid/1410584565601035653","@type":"Researcher","foaf:name":[{"@language":"en","@value":"MIYAMURA KAZUYOSHI"},{"@language":"ja","@value":"宮村 知良"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"大分県農林水産研究指導センター水産研究部"},{"@language":"en","@value":"Fisheries Research Division, Oita Prefectural Agriculture Forestry and Fisheries Research Center"}]},{"@id":"https://cir.nii.ac.jp/crid/1410584565601035655","@type":"Researcher","foaf:name":[{"@language":"en","@value":"YAMATOGI TOSHIFUMI"},{"@language":"ja","@value":"山砥 稔文"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"長崎県総合水産試験場"},{"@language":"en","@value":"Nagasaki Prefectural Institute of 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