Nitric Oxide Detoxification by Mesorhizobium loti Affects Root Nodule Symbiosis with Lotus japonicus

DOI Web Site Web Site PubMed 参考文献63件 オープンアクセス
  • Fukudome Mitsutaka
    Graduate School of Science and Engineering, Kagoshima University Division of Symbiotic Systems, National Institute for Basic Biology
  • Shimokawa Yuta
    Graduate School of Science and Engineering, Kagoshima University
  • Hashimoto Shun
    Graduate School of Science and Engineering, Kagoshima University
  • Maesako Yusuke
    Graduate School of Science and Engineering, Kagoshima University
  • Uchi-Fukudome Nahoko
    Graduate School of Science and Engineering, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima University
  • Niihara Kota
    Graduate School of Science and Engineering, Kagoshima University
  • Osuki Ken-ichi
    Graduate School of Science and Engineering, Kagoshima University
  • Uchiumi Toshiki
    Graduate School of Science and Engineering, Kagoshima University

書誌事項

タイトル別名
  • Nitric Oxide Detoxification by <i>Mesorhizobium loti</i> Affects Root Nodule Symbiosis with <i>Lotus japonicus</i>

この論文をさがす

説明

<p>Root nodule symbiosis between legumes and rhizobia involves nitric oxide (NO) regulation by both the host plant and symbiotic rhizobia. However, the mechanisms by which the rhizobial control of NO affects root nodule symbiosis in Lotus japonicus are unknown. Therefore, we herein investigated the effects of enhanced NO removal by Mesorhizobium loti on symbiosis with L. japonicus. The hmp gene, which in Sinorhizobium meliloti encodes a flavohemoglobin involved in NO detoxification, was introduced into M. loti to generate a transconjugant with enhanced NO removal. The symbiotic phenotype of the transconjugant with L. japonicus was examined. The transconjugant showed delayed infection and higher nitrogenase activity in mature nodules than the wild type, whereas nodule senescence was normal. This result is in contrast to previous findings showing that enhanced NO removal in L. japonicus by class 1 phytoglobin affected nodule senescence. To evaluate differences in NO detoxification between M. loti and L. japonicus, NO localization in nodules was investigated. The enhanced expression of class 1 phytoglobin in L. japonicus reduced the amount of NO not only in infected cells, but also in vascular bundles, whereas that of hmp in M. loti reduced the amount of NO in infected cells only. This difference suggests that NO detoxification by M. loti exerts different effects in symbiosis than that by L. japonicus.</p>

収録刊行物

参考文献 (63)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ