To introduce useful characteristics such as fragrance into Argyranthemum frutescens (L.) and to expand the variation, we conducted crosses using A. frutescens as the seed parent and Chamaemelum nobile (L.) All. as the pollen parent. All the tested cross combinations between the three strains of A. frutescens and one strain of C. nobile produced embryos, and healthy plants were obtained by ovule culture. The obtained plantlets had a white ray floret, and the leaf shape was intermediate to those of the parents. The individuals obtained from this cross were subjected to two methods to determine hybridity: flow cytometry analyses and cleaved amplified polymorphic sequence (CAPS) markers. For the CAPS marker, we selected the internal transcribed spacer (ITS) region, which is highly variable among the genera, as the region to be amplified. We selected restriction enzymes BmgT120 I and Afl II, which selectively cut common sequences in the genus Argyranthemum, based on the sequence analysis of one parent strain each of A. frutescens and C. nobile and alignment with known sequences of related species. Flow cytometry analyses and CAPS markers revealed that the individuals obtained from the cross between A. frutescens and C. nobile are intergeneric hybrids. In addition, these established methods were capable of quickly and reliably identifying hybrids between A. frutescens and C. nobile. This report shows for the first time that crossbreeding between A. frutescens (seed parent) and C. nobile (pollen parent) is possible, and further development of Argyranthemum breeding, such as the expansion of variation by intergeneric crosses, is expected.
"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410855511119089540","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Katsuoka Hiroyuki"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Izu Agricultural Research Center, Shizuoka Prefectural Research Institute of Agriculture and Forestry"}]},{"@id":"https://cir.nii.ac.jp/crid/1410855511119089542","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Hamabe Naoya"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Izu Agricultural Research Center, Shizuoka Prefectural Research Institute of Agriculture and Forestry"}]},{"@id":"https://cir.nii.ac.jp/crid/1410855511119089544","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Kato Chiemi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Izu Agricultural Research Center, Shizuoka Prefectural Research Institute of Agriculture and Forestry"}]},{"@id":"https://cir.nii.ac.jp/crid/1410855511119089541","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Hisamatsu Susumu"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Izu Agricultural Research Center, Shizuoka Prefectural Research Institute of Agriculture and Forestry"}]},{"@id":"https://cir.nii.ac.jp/crid/1410855511119089539","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Taneishi Motohiro"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Izu Agricultural Research Center, Shizuoka Prefectural Research Institute of Agriculture and Forestry"}]},{"@id":"https://cir.nii.ac.jp/crid/1410855511119089543","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Sasaki Toshiyuki"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Izu Agricultural Research Center, Shizuoka Prefectural Research Institute of Agriculture and Forestry"}]},{"@id":"https://cir.nii.ac.jp/crid/1410855511119089538","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Baba Fujio"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Izu Agricultural Research Center, Shizuoka Prefectural Research Institute of Agriculture and Forestry"}]},{"@id":"https://cir.nii.ac.jp/crid/1410855511119089536","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Ikegaya Atsushi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Agricultural Production, Shizuoka Professional University Junior College of Agriculture"}]},{"@id":"https://cir.nii.ac.jp/crid/1410855511119089537","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Inaba Zentaro"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Agricultural Production, Shizuoka Professional University Junior College of Agriculture"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13424580"},{"@type":"LISSN","@value":"13424580"},{"@type":"EISSN","@value":"13476114"},{"@type":"CODEN","@value":"PLBIF6"},{"@type":"NDL_BIB_ID","@value":"000000157745"},{"@type":"ISSN","@value":"13424580"},{"@type":"NCID","@value":"AA11250821"}],"prism:publicationName":[{"@language":"en","@value":"Plant Biotechnology"},{"@language":"ja","@value":"Ｐｌａｎｔ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ"},{"@language":"en","@value":"Plant Biotechnology"},{"@language":"ja","@value":"Ｐｌａｎｔ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ"}],"dc:publisher":[{"@language":"en","@value":"Japanese Society for Plant Biotechnology"},{"@language":"ja","@value":"日本植物バイオテクノロジー学会"}],"prism:publicationDate":"2022-06-25","prism:volume":"39","prism:number":"2","prism:startingPage":"93","prism:endingPage":"100"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://id.ndl.go.jp/bib/032272040"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I032272040"},{"@id":"https://www.jstage.jst.go.jp/article/plantbiotechnology/39/2/39_21.1130a/_pdf"}],"availableAt":"2022-06-25","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=%3Ci%3EArgyranthemum%3C/i%3E","dc:title":"Argyranthemum"},{"@id":"https://cir.nii.ac.jp/all?q=CAPS%20marker","dc:title":"CAPS marker"},{"@id":"https://cir.nii.ac.jp/all?q=%3Ci%3EChamaemelum%3C/i%3E","dc:title":"Chamaemelum"},{"@id":"https://cir.nii.ac.jp/all?q=flow%20cytometry","dc:title":"flow cytometry"},{"@id":"https://cir.nii.ac.jp/all?q=intergeneric%20hybridization","dc:title":"intergeneric 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