Sarcocystis cruzi is a member of the genus Sarcocystis, infecting bovine animals such as cattle and bison as intermediate hosts, and canids such as dogs and raccoon dogs as definitive hosts. Acute sarcocystosis of S. cruzi causes occasional symptoms in cattle, including weight loss, reduced milk production, abortions, and death, and similar to other Sarcocystis species can potentially cause food poisoning in humans when raw or undercooked infected cattle meat is consumed. Despite these issues, genetic information on S. cruzi is scarce, and there is no specific quantitative method for the detection and quantification of the parasite in infected cattle. In this study, we aimed to develop a method based on high-throughput sequencing of S. cruzi genome and transcriptome that specifically and quantitatively detects the S. cruzi acetyl-CoA synthetase gene (ScACS). Cardiac muscles were collected from slaughterhouses in Saitama Prefecture to obtain sarcocysts from which DNA and RNA were extracted for the high-throughput sequencing. Using the sequences, we developed a specific quantitative PCR assay which could distinguish S. cruzi ACS from that of Toxoplasma gondii by taking advantage of the differences in their exon/intron organizations and validated the assay with the microscopic counting of the S. cruzi bradyzoites. Thus, this assay will be useful for future studies of S. cruzi pathogenesis in cattle and for the surveillance of infected animals, thereby easing public health concerns.
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Vet. Med. Sci."},{"@language":"en","@value":"The Journal of Veterinary Medical Science"},{"@language":"ja","@value":"Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ"}],"dc:publisher":[{"@language":"en","@value":"JAPANESE SOCIETY OF VETERINARY SCIENCE"},{"@language":"ja","@value":"公益社団法人 日本獣医学会"}],"prism:publicationDate":"2023","prism:volume":"85","prism:number":"1","prism:startingPage":"105","prism:endingPage":"110"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"https://www.jstage.jst.go.jp/article/jvms/85/1/85_22-0481/_pdf"},{"@id":"https://search.jamas.or.jp/link/ui/2024000710"}],"availableAt":"2023","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=acetyl-CoA%20synthetase","dc:title":"acetyl-CoA synthetase"},{"@id":"https://cir.nii.ac.jp/all?q=bradyzoite","dc:title":"bradyzoite"},{"@id":"https://cir.nii.ac.jp/all?q=quantitative%20real-time%20PCR","dc:title":"quantitative real-time PCR"},{"@id":"https://cir.nii.ac.jp/all?q=%3Ci%3ESarcocystis%20cruzi%3C/i%3E","dc:title":"Sarcocystis cruzi"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360016866979585152","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Sarcocystis-infection of cattle in Hungary"}]},{"@id":"https://cir.nii.ac.jp/crid/1360016867531409408","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Multi-omics analysis delineates the distinct functions of sub-cellular acetyl-CoA pools in Toxoplasma gondii"}]},{"@id":"https://cir.nii.ac.jp/crid/1360016867882894976","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Elevated IgG Antibody toSarcocystis CruziAssociated with Eosinophilic Myositis in Cattle"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285706220697472","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Molecular differentiation 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toxin"}]},{"@id":"https://cir.nii.ac.jp/crid/1390005506388838144","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"A Food Poisoning Case by the Deer Meat with Three Kinds of Sarcocystis spp. in Niigata Prefecture, Japan"},{"@language":"ja","@value":"3種のSarcocystisが寄生するシカ肉が原因と推定された食中毒事例"},{"@value":"3種のSarcocystisが寄生するシカ肉が原因と推定された食中毒事例"},{"@language":"ja-Kana","@value":"3シュ ノ Sarcocystis ガ キセイ スル シカニク ガ ゲンイン ト スイテイ サレタ ショクチュウドク ジレイ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679402143232","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"An Outbreak of Suspected Food Poisoning Related to Deer Meat Containing Sarcocystis Cysts"},{"@language":"ja","@value":"シカ肉中のSarcosystisが原因として疑われた有症苦情"},{"@value":"シカ肉中のSarcosystisが原因として疑われた有症苦情"},{"@language":"ja-Kana","@value":"シカニク チュウ ノ Sarcosystis ガ ゲンイン ト シテ ウタガワレタ ユウショウ クジョウ"},{"@value":"An Outbreak of Suspected Food Poisoning Related to Deer Meat Containing Sarcocystis Cysts"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679685468672","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Distribution of Sarcocystis cruzi Cysts in Bovine Striated Muscles"},{"@language":"ja","@value":"Sarcocystis cruziシストの牛筋肉における寄生分布"},{"@value":"Sarcocystis cruziシストの牛筋肉における寄生分布"},{"@language":"ja-Kana","@value":"Sarcocystis cruzi シスト ノ ウシ キンニク ニ オケル キ"},{"@value":"Distribution of Sarcocystis cruzi Cysts in Bovine Striated Muscles"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681402154880","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Experimental Infection of Raccoon Dogs with Sarcocystis cruzi and S. miescheriana."},{"@language":"ja","@value":"Sarcocystis cruziとS. miescherianaによるホンドタヌキへの実験感染"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681402386432","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"PCR-based Discrimination of Toxoplasma gondii from Pigs at an Abattoir in Okinawa, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282752326297216","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"First Case Report of Possible Sarcocystis truncata-induced Food Poisoning in Venison"}]},{"@id":"https://cir.nii.ac.jp/crid/1390565134837668736","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Food Poisoning Incident Caused by the Consumption of Raw Deer Meat Contaminated with Genus Sarcocystis"},{"@language":"ja","@value":"Sarcocystis 属が寄生していた鹿肉を生で喫食したことによる食中毒事例"},{"@value":"Sarcocystis属が寄生していた鹿肉を生で喫食したことによる食中毒事例"},{"@language":"ja-Kana","@value":"Sarcocystisゾク ガ キセイ シテ イタ カニク オ ナマ デ キツショク シタ コトニ ヨル ショクチュウドク ジレイ"}]}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:2010072662"},{"@type":"CROSSREF","@value":"10.1292/jvms.22-0481"},{"@type":"PUBMED","@value":"36450502"},{"@type":"OPENAIRE","@value":"doi_________::647307e37f5dbc75fca13e5b97004932"}]}