{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390866415329014656.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.2208/jscejj.24-25022"}},{"identifier":{"@type":"URI","@value":"https://www.jstage.jst.go.jp/article/jscejj/80/25/80_24-25022/_pdf"}}],"dc:title":[{"@language":"en","@value":"EVALUATION OF ENVIRONMENTAL DNA VARIATION FOR TURBID WATER CONSIDERING DNA STATES"},{"@language":"ja","@value":"DNA存在形態を考慮した濁水における環境DNAの変動の評価"}],"dc:language":"ja","description":[{"type":"abstract","notation":[{"@language":"en","@value":"<p> Although the accuracy of environmental DNA (eDNA) detection is influenced by biological and environmental conditions, eDNA dynamics in turbid water is not well understood, precluding establishment of eDNA surveys in turbid water area. In this study, we evaluated the effect of different turbidity conditions on aqueous eDNA fate with different states. Water samples under five turbidity conditions (0, 20, 50, 100, and 200 ppm) were designed by mixing riverbed sediment and either distilled water or aquarium water rearing common carps (<i>Cyprinus carpio</i>). We filtered the turbid water samples using two types of glass fiber filters with different pore sizes and quantified carp eDNA using digital PCR. Consequently, as turbidity increased, the concentration of eDNA also increased; however, under high turbidity conditions, the eDNA concentration tended to decrease. These findings suggest that eDNA recovery is higher at moderate levels of turbidity owing to the adsorption of eDNA and the influence of PCR inhibitors.</p>"},{"@language":"ja","@value":"<p>　環境DNA（eDNA）の検出精度は生物・環境的な条件に左右されるが，濁水中のeDNAの動態は深く理解されていない．本研究では，濁水条件の違いが存在形態の異なるeDNAの挙動に与える影響を評価した．蒸留水およびコイの水槽水と河川で採取した土砂を混合して目標濁度0，20，50，100，200ppmの濁水条件の水試料を作成した．孔径の異なる2種類のガラス繊維ろ紙を用いてeDNAの濃縮を行い，デジタルPCRによりeDNA濃度を定量した．結果として，濁度の増加に伴いeDNA濃度が高まっていたが，高濁度条件下においてはeDNA濃度が低下する傾向が確認された．これらの事実は，eDNAの吸着とPCR阻害物質の影響により，中程度の濁度においてeDNA濃度が高まることを示唆する．</p>"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410866415329014658","@type":"Researcher","foaf:name":[{"@language":"en","@value":"MIKAMI Yuki"},{"@language":"ja","@value":"三上 優貴"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"宮崎大学大学院 工学研究科"}]},{"@id":"https://cir.nii.ac.jp/crid/1410866415329014656","@type":"Researcher","foaf:name":[{"@language":"en","@value":"XU Chen"},{"@language":"ja","@value":"Xu Chen"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"宮崎大学大学院 工学研究科"}]},{"@id":"https://cir.nii.ac.jp/crid/1410866415329014657","@type":"Researcher","foaf:name":[{"@language":"en","@value":"NUKAZAWA Kei"},{"@language":"ja","@value":"糠澤 桂"}],"jpcoar:affiliationName":[{"@language":"ja","@value":"宮崎大学 工学部工学科土木環境プログラム"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"24366021"}],"prism:publicationName":[{"@language":"en","@value":"Japanese Journal of JSCE"},{"@language":"ja","@value":"土木学会論文集"},{"@language":"en","@value":"Japanese Journal of JSCE"},{"@language":"ja","@value":"土木学会論文集"}],"dc:publisher":[{"@language":"en","@value":"Japan Society of Civil Engineers"},{"@language":"ja","@value":"公益社団法人 土木学会"}],"prism:publicationDate":"2024","prism:volume":"80","prism:number":"25","prism:startingPage":"n/a"},"reviewed":"false","url":[{"@id":"https://www.jstage.jst.go.jp/article/jscejj/80/25/80_24-25022/_pdf"}],"availableAt":"2024","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Cyprinus%20Carpio","dc:title":"Cyprinus Carpio"},{"@id":"https://cir.nii.ac.jp/all?q=Environmental%20DNA","dc:title":"Environmental DNA"},{"@id":"https://cir.nii.ac.jp/all?q=Digital%20PCR","dc:title":"Digital PCR"},{"@id":"https://cir.nii.ac.jp/all?q=Turbid%20water","dc:title":"Turbid water"},{"@id":"https://cir.nii.ac.jp/all?q=Adsorption","dc:title":"Adsorption"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360011143956444800","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The effect of dilution and the use of a post-extraction nucleic acid purification column on the accuracy, precision, and inhibition of environmental DNA samples"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011146457804800","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Mechanisms and Regulation of Extracellular DNA Release and Its Biological Roles in Microbial Communities"}]},{"@id":"https://cir.nii.ac.jp/crid/1360013170335265664","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Environmental conditions influence eDNA particle size distribution in aquatic systems"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021392662393728","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Persistence and degradation dynamics of eDNA affected by environmental factors in aquatic ecosystems"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021393788280320","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The effects of humic substances on DNA isolation from 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