Fucoidan, a high-molecular weight sulfated polysaccharide in brown algae, has been reported to be effective in the enhancement of immune responses to tumors and infections. On the other hand, it is also known to have anti-inflammatory effects, and thought to help maintain immune homeostasis. In this study, the efficacy of CUA fucoidan which consists of fucoidan from Okinawamozuku, fucoidan from Mekabu and Agaricus mycelium extract in prevention of pneumonia caused by viral infection was evaluated. In this study, TLR ligands solution (TLR3-L: 62.5 µg poly(I:C), TLR4-L: 25 µg rSARS-CoV-2 spike protein, TLR7-L: 5.0 µg gardiquimod) was administered orally to C57BL/6J mice to create a mouse model of pneumonia. After 2 weeks of acclimatization, 30 mg CUA fucoidan was orally administered for 2 weeks, and pneumonia was induced by the above-mentioned infectious stimuli, and the pathological signs were analyzed and evaluated after 2 days. A remarkable inflammatory sign such as tissue thickening and monocyte infiltration was occurred in the control mice, whereas a remarkable alleviation of pulmonary inflammation was observed in the mice treated with CUA fucoidan. In this situation, the levels of IL-6, a key inflammatory cytokine, in alveolar lavage (BAL) fluid and plasma were significantly lower in the fucoidan group as compared with the control group. Interestingly, IFN-α and IFN-γ levels in BAL fluid were not reduced by CUA fucoidan treatment. In addition, the expression of class II MHC molecules in leukocytes was significantly reduced in the fucoidan-pretreated mice. These results suggest that continuous intake of CUA fucoidan may contribute to the prevention of inflammation caused by upper respiratory infections.
"},{"@language":"ja","@value":"【目的】 褐藻類に含まれる高分子硫酸化多糖フコイダンは, 自然免疫細胞を活性化し, 腫瘍および感染免疫応答を増強することが報告されている. 一方で, 大腸等で生じる炎症に対して抑制効果を発揮することも知られており, 免疫機構の恒常性維持に働くことが期待される. 本研究では, ウイルス感染で生じる重症肺炎に対する高分子CUAフコイダン（オキナワモズク由来フコイダン, メカブ由来フコイダンおよびアガリクス菌糸体エキス末を混合した食品素材）の発症予防効果を検証した. 【方法】 本試験では, C57BL/6JマウスにTLRリガンド溶液（TLR3-L: 62.5 µg Poly(I:C), TLR4-L: 25 µg リコンビナントSARS-CoV-2スパイクタンパク質, TLR7-L: 5.0 µg Gardiquimod）を口腔咽頭投与することで肺炎モデルマウスを作成した. 2週間の馴化飼育後に, 30 mgのCUAフコイダン（対照群には, 同量の水）を2週間連日経口投与した後, 上記の感染刺激により肺炎を誘導して2日後の炎症病態を解析・評価した. 【結果】 肺炎を誘導した対照群マウスの肺では, 組織肥厚や単球浸潤などの顕著な炎症像が観察されたのに対し, CUAフコイダンを経口投与したマウス（フコイダン群）では, 肺組織における明らかな炎症緩和を認めた. また, 肺胞洗浄（BAL）液および血漿中の炎症性サイトカイン濃度を測定した結果, フコイダン群のIL-6産生量は対照群と比べて有意に低値を示した. 一方, BAL液中のIFN-αおよびIFN-γ濃度では, CUAフコイダン投与による低下を認めなかった. 加えて, BAL液中の白血球におけるClass II MHC分子の発現上昇は, フコイダン群と比較して対照群で顕著に上昇した. 以上, CUAフコイダンの継続摂取は, 上気道感染症における炎症（肺炎およびサイトカインストーム）の発症予防に寄与する可能性が示された.
"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410868151302872960","@type":"Researcher","foaf:name":[{"@language":"ja","@value":"宮崎 義之"},{"@language":"en","@value":"Miyazaki Yoshiyuki"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Kyushu University"},{"@language":"ja","@value":"九州大・院・農"},{"@language":"en","@value":"NPO Res. Inst. Fucoidan"},{"@language":"ja","@value":"NPOフコイダン研究所"}]},{"@id":"https://cir.nii.ac.jp/crid/1410868151302872962","@type":"Researcher","foaf:name":[{"@language":"ja","@value":"中野 勇人"},{"@language":"en","@value":"Nakano Hayato"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Ventuno Co., Ltd."},{"@language":"ja","@value":"（株）ヴェントゥーノ"}]},{"@id":"https://cir.nii.ac.jp/crid/1410868151302872964","@type":"Researcher","foaf:name":[{"@language":"ja","@value":"竹内 秀吾"},{"@language":"en","@value":"Takeuchi Shugo"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Kaisou-sci. Corp."},{"@language":"ja","@value":"（株）海藻サイエンスの会"}]},{"@id":"https://cir.nii.ac.jp/crid/1410868151302872961","@type":"Researcher","foaf:name":[{"@language":"ja","@value":"竹内 秀晃"},{"@language":"en","@value":"Takeuchi Hideaki"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Kamerycah Inc."},{"@language":"ja","@value":"Kamerycah Inc."}]},{"@id":"https://cir.nii.ac.jp/crid/1410868151302872963","@type":"Researcher","foaf:name":[{"@language":"ja","@value":"立川 大介"},{"@language":"en","@value":"Tachikawa Daisuke"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Kyushu University"},{"@language":"ja","@value":"九州大・院・農"},{"@language":"ja","@value":"NPOフコイダン研究所"},{"@language":"en","@value":"NPO Res. Inst. Fucoidan"},{"@language":"en","@value":"Wakamiya Hospital"},{"@language":"ja","@value":"（医法）若宮病院"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"27593843"}],"prism:publicationName":[{"@language":"ja","@value":"日本食品科学工学会大会講演要旨集"},{"@language":"en","@value":"Abstracts of the Annual Meeting of Japanese Society for Food Science and Technology"}],"dc:publisher":[{"@language":"en","@value":"Japanese Society for Food Science and Technology"},{"@language":"ja","@value":"公益社団法人 日本食品科学工学会"}],"prism:publicationDate":"2024","prism:volume":"71","prism:number":"0","prism:startingPage":"288"},"reviewed":"false","jpcoar:conferenceName":"日本食品科学工学会第71回大会","jpcoar:conferencePlace":"名城大学","url":[{"@id":"https://www.jstage.jst.go.jp/article/aamjsfst/71/0/71_288/_pdf"}],"availableAt":"2024","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=%E3%83%95%E3%82%B3%E3%82%A4%E3%83%80%E3%83%B3","dc:title":"フコイダン"},{"@id":"https://cir.nii.ac.jp/all?q=%E7%A1%AB%E9%85%B8%E5%8C%96%E5%A4%9A%E7%B3%96%E9%A1%9E","dc:title":"硫酸化多糖類"},{"@id":"https://cir.nii.ac.jp/all?q=%E6%B5%B7%E8%97%BB%E6%88%90%E5%88%86","dc:title":"海藻成分"},{"@id":"https://cir.nii.ac.jp/all?q=%E4%B8%8A%E6%B0%97%E9%81%93%E6%84%9F%E6%9F%93","dc:title":"上気道感染"},{"@id":"https://cir.nii.ac.jp/all?q=%E6%8A%97%E7%82%8E%E7%97%87%E4%BD%9C%E7%94%A8","dc:title":"抗炎症作用"},{"@id":"https://cir.nii.ac.jp/all?q=Fucoidan","dc:title":"Fucoidan"},{"@id":"https://cir.nii.ac.jp/all?q=Sulfated%20polysaccharides","dc:title":"Sulfated polysaccharides"},{"@id":"https://cir.nii.ac.jp/all?q=Seaweed%20components","dc:title":"Seaweed components"},{"@id":"https://cir.nii.ac.jp/all?q=upper%20respiratory%20infection","dc:title":"upper respiratory infection"},{"@id":"https://cir.nii.ac.jp/all?q=anti-inflammatory%20effects","dc:title":"anti-inflammatory effects"}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:2014517350"},{"@type":"CROSSREF","@value":"10.3136/aamjsfst.71.0_288"}]}