Effect of Shakuyaku-Kanzo-To on Serum Estrogen Levels and Adrenal Gland Cells in Ovariectomized Rats

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  • KATO,Tomoyasu
    Department of Endocrinology, Medical Research Institute, Tokyo Medical and Dental University
  • OKAMOTO,Ryouhei
    Department of Endocrinology, Medical Research Institute, Tokyo Medical and Dental University

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  • 芍薬甘草湯の去勢メスラット血清エストロゲン値並びに培養副腎細胞に及ぼす影響 : 内因性エストロゲン増加の基礎検討

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The aim of this study is to investigate the effect of the traditional herbal medicine, Shakuyaku. Kanzo-To (SKT) on oophorectomized (OVX) rats. In an in vivo experiment, OVX rats were orally given SKT (low dose group: 45mg/kg b.w./day, high dose group: 450mg/kg b.w./day) every day for 2 weeks. Serum dehydroepiandrosterone-sulfate (DHEA-S) and estrone (E_1) levels of the low and high dose groups were higher than those of the control given vehicle. Serum 17β-estradiol (E_2) levels only in the low dose group were higher than those of the control. In an in vitro experiment, adrenal gland cells of OVX rats were incubated with media containing SKT or Shakuyaku (S) or Kanzo (K) for 72-h. DHEA-S production was increased in the SKT 1,000 pg/ml and K 500μg/ml group in comparison with the control. At the conclusion of this period, the cells were reincubated for an additional 8-h interval in an androstenedione (Δ^4-A, 10^<-7> M)-supplemented medium. Aromatase activity assessed by the conversion of Δ^4-A to E_1 or E_2 was not increased in the SKT 1,000μg/ml group. These results indicate that SKT increases DHEA-S, a precursor of estrogen, and brings about an increase in serum estrogen concentrations in OVX rats.

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