DETERMINATION OF FREE AND ESTERIFIED RIBOFLAVIN BY LUMIFLAVIN METHOD

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Description

1. The improved lumiflavin method for estimating free and -esterified B2 is described in detail.<br> 2. The lumiflavin method is exact, reproducible and specific for B2, the recoveries being always practically cemplete and the blank fluorescence can be completely removed by permanganate treatment.<br> 3. Free and esterified B2 are equally converted to lumiiavin without preliminary enzymatic hydrolysis.<br> 4. The distribution of free and esterified B2 in animal and vegetable tissues is reported.

Journal

  • J Biochem (Tokyo)

    J Biochem (Tokyo) 37 (4), 445-458, 1950

    The Japanese Biochemical Society

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