{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1871428067455088640.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.5115/acb.21.202"}},{"identifier":{"@type":"PMID","@value":"35466086"}}],"dc:title":[{"@value":"A study on the effect of JNJ-10397049 on proliferation and differentiation of neural precursor cells"}],"description":[{"notation":[{"@value":"The orexin 2 receptor plays a central role in maintaining sleep and wakefulness. Recently, it has been shown that sleep and wakefulness orchestrate the proliferation and differentiation of oligodendrocytes. Here, we explored the role of a selective orexin 2 receptor antagonist (JNJ-10397049) in proliferation and differentiation of neural progenitor cells (NPCs). We evaluated the proliferation potential of NPCs after exposure to different concentrations of JNJ-10397049 by using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide and neurosphere assays. Moreover, the expression of differentiation markers was assessed by immunocytochemistry and real-time polymerase chain reaction. JNJ-10397049 significantly increased the proliferation of NPCs at lower concentrations. In addition, orexin 2 receptor antagonist facilitated progression of differentiation of NPCs towards oligodendroglial lineage by considerable expression of Olig2 and 2',3'-cyclic-nucleotide 3'-phosphodiesterase as well as decreased expression of nestin marker. The results open a new avenue for future investigations in which the production of more oligodendrocytes from NPCs is needed."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1891428067455088768","@type":"Researcher","foaf:name":[{"@value":"Neda Karami"}]},{"@id":"https://cir.nii.ac.jp/crid/1891428067455088641","@type":"Researcher","foaf:name":[{"@value":"Hassan Azari"}]},{"@id":"https://cir.nii.ac.jp/crid/1891428067455088769","@type":"Researcher","foaf:name":[{"@value":"Moosa Rahimi"}]},{"@id":"https://cir.nii.ac.jp/crid/1891428067455088770","@type":"Researcher","foaf:name":[{"@value":"Hadi Aligholi"}]},{"@id":"https://cir.nii.ac.jp/crid/1891428067455088640","@type":"Researcher","foaf:name":[{"@value":"Tahereh Kalantari"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"20933665"},{"@type":"EISSN","@value":"20933673"}],"prism:publicationName":[{"@value":"Anatomy & Cell Biology"}],"dc:publisher":[{"@value":"Korean Association of Anatomists"}],"prism:publicationDate":"2022-04-25","prism:volume":"55","prism:startingPage":"179","prism:endingPage":"189"},"dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Original%20Article","dc:title":"Original Article"}],"dataSourceIdentifier":[{"@type":"OPENAIRE","@value":"doi_dedup___::15d654a1b6b49f476c5aec69dbcc89b7"}]}