{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1871428067603025280.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/irv.12221"}},{"identifier":{"@type":"PMID","@value":"24373385"}}],"dc:title":[{"@value":"Modified vaccinia virus <scp>A</scp>nkara expressing the hemagglutinin of pandemic (<scp>H</scp>1<scp>N</scp>1) 2009 virus induces cross‐protective immunity against <scp>E</scp>urasian ‘avian‐like’ <scp>H</scp>1<scp>N</scp>1 swine viruses in mice"}],"description":[{"notation":[{"@value":"<jats:sec><jats:title>Objectives</jats:title><jats:p>To examine cross‐reactivity between hemagglutinin (<jats:styled-content style=\"fixed-case\">HA</jats:styled-content>) derived from A/California/7/09 (<jats:styled-content style=\"fixed-case\">CA</jats:styled-content>/09) virus and that derived from representative Eurasian “avian‐like” (<jats:styled-content style=\"fixed-case\">EA</jats:styled-content>) H1N1 swine viruses isolated in Italy between 1999 and 2008 during virological surveillance in pigs.</jats:p></jats:sec><jats:sec><jats:title>Design</jats:title><jats:p>Modified vaccinia virus <jats:styled-content style=\"fixed-case\">A</jats:styled-content>nkara (<jats:styled-content style=\"fixed-case\">MVA</jats:styled-content>) expressing the <jats:styled-content style=\"fixed-case\">HA</jats:styled-content> gene of <jats:styled-content style=\"fixed-case\">CA</jats:styled-content>/09 virus (<jats:styled-content style=\"fixed-case\">MVA</jats:styled-content>‐<jats:styled-content style=\"fixed-case\">HA</jats:styled-content>‐<jats:styled-content style=\"fixed-case\">CA</jats:styled-content>/09) was used as a vaccine to investigate cross‐protective immunity against H1N1 swine viruses in mice.</jats:p></jats:sec><jats:sec><jats:title>Sample</jats:title><jats:p>Two classical swine H1N1 (<jats:styled-content style=\"fixed-case\">CS</jats:styled-content>) viruses and four representative <jats:styled-content style=\"fixed-case\">EA</jats:styled-content>‐like H1N1 swine viruses previously isolated during outbreaks of respiratory disease in pigs on farms in Northern Italy were used in this study.</jats:p></jats:sec><jats:sec><jats:title>Setting</jats:title><jats:p>Female C57<jats:styled-content style=\"fixed-case\">BL</jats:styled-content>/6 mice were vaccinated with <jats:styled-content style=\"fixed-case\">MVA</jats:styled-content>/<jats:styled-content style=\"fixed-case\">HA</jats:styled-content>/<jats:styled-content style=\"fixed-case\">CA</jats:styled-content>/09 and then challenged intranasally with H1N1 swine viruses.</jats:p></jats:sec><jats:sec><jats:title>Main outcome measures</jats:title><jats:p>Cross‐reactive antibody responses were determined by hemagglutination‐ inhibition (<jats:styled-content style=\"fixed-case\">HI</jats:styled-content>) and virus microneutralizing (<jats:styled-content style=\"fixed-case\">MN</jats:styled-content>) assays of sera from <jats:styled-content style=\"fixed-case\">MVA</jats:styled-content>‐vaccinated mice. The extent of protective immunity against infection with H1N1 swine viruses was determined by measuring lung viral load on days 2 and 4 post‐challenge.</jats:p></jats:sec><jats:sec><jats:title>Results and Conclusions</jats:title><jats:p>Systemic immunization of mice with <jats:styled-content style=\"fixed-case\">CA</jats:styled-content>/09‐derived <jats:styled-content style=\"fixed-case\">HA</jats:styled-content>, vectored by <jats:styled-content style=\"fixed-case\">MVA</jats:styled-content>, elicited cross‐protective immunity against recent <jats:styled-content style=\"fixed-case\">EA</jats:styled-content>‐like swine viruses. This immune protection was related to the levels of cross‐reactive <jats:styled-content style=\"fixed-case\">HI</jats:styled-content> antibodies in the sera of the immunized mice and was dependent on the similarity of the antigenic site Sa of H1 <jats:styled-content style=\"fixed-case\">HA</jats:styled-content>s. Our findings suggest that the herd immunity elicited in humans by the pandemic (H1N1) 2009 virus could limit the transmission of recent <jats:styled-content style=\"fixed-case\">EA</jats:styled-content>‐like swine <jats:styled-content style=\"fixed-case\">HA</jats:styled-content> genes into the influenza A virus gene pool in humans.</jats:p></jats:sec>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1891428067603025291","@type":"Researcher","foaf:name":[{"@value":"Paolo Cordioli"}]},{"@id":"https://cir.nii.ac.jp/crid/1891428067603025284","@type":"Researcher","foaf:name":[{"@value":"Marzia Facchini"}]},{"@id":"https://cir.nii.ac.jp/crid/1891428067603025282","@type":"Researcher","foaf:name":[{"@value":"Concetta Fabiani"}]},{"@id":"https://cir.nii.ac.jp/crid/1891428067603025280","@type":"Researcher","foaf:name":[{"@value":"Maria R. Castrucci"}]},{"@id":"https://cir.nii.ac.jp/crid/1891428067603025285","@type":"Researcher","foaf:name":[{"@value":"Isabella Donatelli"}]},{"@id":"https://cir.nii.ac.jp/crid/1891428067603025283","@type":"Researcher","foaf:name":[{"@value":"Yoshihiro Kawaoka"}]},{"@id":"https://cir.nii.ac.jp/crid/1891428067603025286","@type":"Researcher","foaf:name":[{"@value":"Maria Alessandra De Marco"}]},{"@id":"https://cir.nii.ac.jp/crid/1891428067603025290","@type":"Researcher","foaf:name":[{"@value":"Ester Sciaraffia"}]},{"@id":"https://cir.nii.ac.jp/crid/1891428067603025287","@type":"Researcher","foaf:name":[{"@value":"Antonio Siccardi"}]},{"@id":"https://cir.nii.ac.jp/crid/1891428067603025289","@type":"Researcher","foaf:name":[{"@value":"Giuseppina Di Mario"}]},{"@id":"https://cir.nii.ac.jp/crid/1891428067603025281","@type":"Researcher","foaf:name":[{"@value":"Bruno Garulli"}]},{"@id":"https://cir.nii.ac.jp/crid/1891428067603025288","@type":"Researcher","foaf:name":[{"@value":"Monica Meola"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"17502640"},{"@type":"EISSN","@value":"17502659"}],"prism:publicationName":[{"@value":"Influenza and Other Respiratory Viruses"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2013-12-23","prism:volume":"8","prism:startingPage":"367","prism:endingPage":"375"},"dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Swine%20Diseases","dc:title":"Swine Diseases"},{"@id":"https://cir.nii.ac.jp/all?q=Swine","dc:title":"Swine"},{"@id":"https://cir.nii.ac.jp/all?q=Cross%20Protection","dc:title":"Cross Protection"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Expression","dc:title":"Gene Expression"},{"@id":"https://cir.nii.ac.jp/all?q=Hemagglutinin%20Glycoproteins,%20Influenza%20Virus","dc:title":"Hemagglutinin Glycoproteins, Influenza Virus"},{"@id":"https://cir.nii.ac.jp/all?q=Vaccinia%20virus","dc:title":"Vaccinia virus"},{"@id":"https://cir.nii.ac.jp/all?q=Original%20Articles","dc:title":"Original Articles"},{"@id":"https://cir.nii.ac.jp/all?q=Mice,%20Inbred%20C57BL","dc:title":"Mice, Inbred C57BL"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Influenza%20A%20Virus,%20H1N1%20Subtype","dc:title":"Influenza A Virus, H1N1 Subtype"},{"@id":"https://cir.nii.ac.jp/all?q=Influenza%20A%20virus","dc:title":"Influenza A virus"},{"@id":"https://cir.nii.ac.jp/all?q=Influenza%20Vaccines","dc:title":"Influenza Vaccines"},{"@id":"https://cir.nii.ac.jp/all?q=Influenza,%20Human","dc:title":"Influenza, Human"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Female","dc:title":"Female"}],"dataSourceIdentifier":[{"@type":"OPENAIRE","@value":"doi_dedup___::09b79baf112dd4975bf15f17b8abe29d"}]}