{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1871709542593567488.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/s0923-1811(98)00035-8"}},{"identifier":{"@type":"DOI","@value":"10.5281/zenodo.3375345"}},{"identifier":{"@type":"PMID","@value":"9865447"}},{"identifier":{"@type":"DOI","@value":"10.5281/zenodo.3375346"}}],"dc:title":[{"@value":"Differential effect of etretinate on proliferation and extracellular matrix metabolism of human dermal fibroblasts from elderly and young individuals in a novel three-dimensional culture"}],"description":[{"notation":[{"@value":"The effect of etretinate on proliferation and biosynthesiso f collagensa nd glycosaminoglycans(G AGS) were<br> investigatedu sing human dermal fibroblasts in a novel three-dimensionacl ulture supplementedw ith L-ascorbica cid<br> 2-phosphate. Fibroblasts from two young and two elderly individuals were studied at different concentrations of<br> etretinate, 0.25, 1. O and 2.5 pg/ml. Collagens( hydroxyproline) and GAGS (disaccharideu nits) extracted from the cell<br> layer were analyzed and quantified biochemically by high-performance liquid chromatography (HPLC). Etretinate<br> showed no significant effect on fibroblast proliferation either in the monolayer or the three-dimensionacl ulture.<br> Etretinate increasedth e collageno r GAGS content in the cell layer, which was prominent at etretinate concentrations<br> of 1.0 and 2.5 or 0.25 pg/ml, respectivelyi n fibroblastsf rom the elderly (P < 0.05). This effect was not seenin dermal<br> fibroblast from the young. Theser esultss uggesth at etretinate may have the differential effect on collagena nd GAG<br> metabolismsd ependingo n the donor age of the cultured fibroblasts.0 1998E lsevier ScienceI reland Ltd. All rights<br> reserved."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1891709542593567489","@type":"Researcher","foaf:name":[{"@value":"Khine Khine Zaw"}]},{"@id":"https://cir.nii.ac.jp/crid/1891709542593567490","@type":"Researcher","foaf:name":[{"@value":"Yoshiki Miyachi"}]},{"@id":"https://cir.nii.ac.jp/crid/1891709542593567488","@type":"Researcher","foaf:name":[{"@value":"Osamu Ishikawa"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09231811"}],"prism:publicationName":[{"@value":"Journal of Dermatological Science"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"1998-12-01","prism:volume":"18","prism:startingPage":"155","prism:endingPage":"162"},"dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Aging","dc:title":"Aging"},{"@id":"https://cir.nii.ac.jp/all?q=Fibroblasts","dc:title":"Fibroblasts"},{"@id":"https://cir.nii.ac.jp/all?q=https://zenodo.org/deposit/new","dc:title":"https://zenodo.org/deposit/new"},{"@id":"https://cir.nii.ac.jp/all?q=Extracellular%20Matrix","dc:title":"Extracellular Matrix"},{"@id":"https://cir.nii.ac.jp/all?q=Keratolytic%20Agents","dc:title":"Keratolytic Agents"},{"@id":"https://cir.nii.ac.jp/all?q=Etretinate","dc:title":"Etretinate"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Collagen","dc:title":"Collagen"},{"@id":"https://cir.nii.ac.jp/all?q=Child","dc:title":"Child"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Division","dc:title":"Cell Division"},{"@id":"https://cir.nii.ac.jp/all?q=Cells,%20Cultured","dc:title":"Cells, Cultured"},{"@id":"https://cir.nii.ac.jp/all?q=Aged","dc:title":"Aged"},{"@id":"https://cir.nii.ac.jp/all?q=Glycosaminoglycans","dc:title":"Glycosaminoglycans"},{"@id":"https://cir.nii.ac.jp/all?q=Skin","dc:title":"Skin"}],"dataSourceIdentifier":[{"@type":"OPENAIRE","@value":"doi_dedup___::c47d5f7d11965c66327d37caff51546d"}]}