{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1872272492405873280.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/0006-291x(87)91546-4"}},{"identifier":{"@type":"PMID","@value":"3038090"}}],"dc:title":[{"@value":"Neurotensin receptors on the rat liver plasma membranes"}],"description":[{"notation":[{"@value":"Neurotensin (NT) is now classified as a brain-gut peptide in the central nervous system and gastrointestinal tract. In the present study, we characterized the NT receptors on the rat liver plasma membranes. The specific binding of [3H]NT was time dependent, reversible, and saturable. Scatchard analysis of the specific binding data yielded two classes of binding sites, a high affinity site and a low affinity site. The average maximum number of binding sites (Bmax) amounted to 13.3 +/- 1.1 fmol/mg protein at high affinity site and 122.3 +/- 21.5 fmol/mg protein at low affinity site, respectively. The dissociation constant (Kd) had values of 0.39 +/- 0.01 nM at high affinity site and 8.1 +/- 1.1 nM at low affinity site, respectively. The amount of specifically bound [3H]NT was significantly reduced in the presence of mono and divalent cations, EDTA, EGTA and a peptidase inhibitor bacitracin, NT1-13 competed with [3H]NT for its binding site with an IC50 of 0.19 nM at high affinity site (0.2 nM concentration of [3H]NT) and 0.7 nM at low affinity site (4.0 nM concentration of [3H]NT). Xenopsin, a NT analogue separated from the skin of Xenopus laevis, was equipotent (IC50 0.75 nM) with NT1-13 at 4.0 nM concentration of [3H]NT. C-terminal sequence of NT contains the structure necessary for interaction with NT binding sites whereas N-terminal sequence had no binding activity. Since NT has a hyperglysemic and a hypercholesterolemic effects in rats, these NT receptors on the rat liver plasma membranes may be involved in the hyperglycemia and/or hypercholesteroremia induced by NT."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1892272492405873283","@type":"Researcher","foaf:name":[{"@value":"Koutarou Muraki"}]},{"@id":"https://cir.nii.ac.jp/crid/1892272492405873281","@type":"Researcher","foaf:name":[{"@value":"Hiroo Shikata"}]},{"@id":"https://cir.nii.ac.jp/crid/1892272492405873288","@type":"Researcher","foaf:name":[{"@value":"Masaru Arai"}]},{"@id":"https://cir.nii.ac.jp/crid/1892272492405873289","@type":"Researcher","foaf:name":[{"@value":"Kazuhiro Ueda"}]},{"@id":"https://cir.nii.ac.jp/crid/1892272492405873287","@type":"Researcher","foaf:name":[{"@value":"Yoshihiro Nakata"}]},{"@id":"https://cir.nii.ac.jp/crid/1892272492405873285","@type":"Researcher","foaf:name":[{"@value":"Yoshikazu Nishi"}]},{"@id":"https://cir.nii.ac.jp/crid/1892272492405873284","@type":"Researcher","foaf:name":[{"@value":"Tomio Segawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1892272492405873286","@type":"Researcher","foaf:name":[{"@value":"Noboru Yanaihara"}]},{"@id":"https://cir.nii.ac.jp/crid/1892272492405873280","@type":"Researcher","foaf:name":[{"@value":"Haruaki Yajima"}]},{"@id":"https://cir.nii.ac.jp/crid/1892272492405873282","@type":"Researcher","foaf:name":[{"@value":"Norio Kubo"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"0006291X"}],"prism:publicationName":[{"@value":"Biochemical and Biophysical Research Communications"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"1987-06-01","prism:volume":"145","prism:startingPage":"1071","prism:endingPage":"1079"},"foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Male","dc:title":"Male"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Membrane","dc:title":"Cell Membrane"},{"@id":"https://cir.nii.ac.jp/all?q=Rats,%20Inbred%20Strains","dc:title":"Rats, Inbred Strains"},{"@id":"https://cir.nii.ac.jp/all?q=Binding,%20Competitive","dc:title":"Binding, Competitive"},{"@id":"https://cir.nii.ac.jp/all?q=Rats","dc:title":"Rats"},{"@id":"https://cir.nii.ac.jp/all?q=Receptors,%20Neurotransmitter","dc:title":"Receptors, Neurotransmitter"},{"@id":"https://cir.nii.ac.jp/all?q=Kinetics","dc:title":"Kinetics"},{"@id":"https://cir.nii.ac.jp/all?q=Liver","dc:title":"Liver"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Receptors,%20Neurotensin","dc:title":"Receptors, Neurotensin"},{"@id":"https://cir.nii.ac.jp/all?q=Amino%20Acid%20Sequence","dc:title":"Amino Acid Sequence"},{"@id":"https://cir.nii.ac.jp/all?q=Neurotensin","dc:title":"Neurotensin"}],"dataSourceIdentifier":[{"@type":"OPENAIRE","@value":"doi_dedup___::d2850b846bef59ba62ac9c9ad590ea5e"}]}